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Genome-Wide Identification of the MYB Gene Family and Screening of Potential Genes Involved in Fatty Acid Biosynthesis in Walnut

Dongxue Su1, Jiarui Zheng1, Yuwei Yi1, Shuyuan Zhang1, Luxin Feng1, Danzeng Quzhen2, De Qiong3, Weiwei Zhang1, Qijian Wang1, Feng Xu1,*

1 College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025, China
2 Lhasa Municipal Forestry and Grassland Bureau, Lhasa, 850000, China
3 Agro-Tech Extension and Service Center, Shannan, 856000, China

* Corresponding Author: Feng Xu. Email: email

(This article belongs to the Special Issue: Plant Secondary Metabolism and Functional Biology)

Phyton-International Journal of Experimental Botany 2024, 93(9), 2317-2337. https://doi.org/10.32604/phyton.2024.055350

Abstract

The multifaceted roles of MYB transcriptional regulators are pivotal in orchestrating the complex processes of secondary metabolism, stress tolerance mechanisms, and life cycle progression and development. This study extensively examined the JrMYB genes using whole genome and transcriptomic data, focusing on identifying putative MYB genes associated with fatty acid metabolism. 126 MYB genes were identified within the walnut genome, characterized by hydrophilic proteins spanning lengths ranging from 78 to 1890 base pairs. Analysis of cis-acting elements within the promoter regions of MYB genes revealed many elements linked to cell development, environmental stress, and phytohormones. Transcriptomic data was utilized to examine the role of JrMYB genes in the biosynthesis of fatty acids in walnuts. The results revealed diverse expression of these genes across various tissue sites, displaying varying levels and distinct expression patterns. Furthermore, by integrating the results of the phylogenetic tree with the correlation of expression levels, a total of 10 genes potentially involved in the regulation of fatty acid synthesis were screened. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis was conducted on these 10 genes and further identified 4 candidate genes, and a transcription regulatory network involved in fatty acids metabolism was constructed. This study presents a systematic analysis of JrMYB genes, laying the groundwork for an in-depth exploration of the JrMYB genes family’s function in regulating fatty acid synthesis.

Graphic Abstract

Genome-Wide Identification of the <i>MYB</i> Gene Family and Screening of Potential Genes Involved in Fatty Acid Biosynthesis in Walnut

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APA Style
Su, D., Zheng, J., Yi, Y., Zhang, S., Feng, L. et al. (2024). Genome-wide identification of the myb gene family and screening of potential genes involved in fatty acid biosynthesis in walnut. Phyton-International Journal of Experimental Botany, 93(9), 2317-2337. https://doi.org/10.32604/phyton.2024.055350
Vancouver Style
Su D, Zheng J, Yi Y, Zhang S, Feng L, Quzhen D, et al. Genome-wide identification of the myb gene family and screening of potential genes involved in fatty acid biosynthesis in walnut. Phyton-Int J Exp Bot. 2024;93(9):2317-2337 https://doi.org/10.32604/phyton.2024.055350
IEEE Style
D. Su et al., "Genome-Wide Identification of the MYB Gene Family and Screening of Potential Genes Involved in Fatty Acid Biosynthesis in Walnut," Phyton-Int. J. Exp. Bot., vol. 93, no. 9, pp. 2317-2337. 2024. https://doi.org/10.32604/phyton.2024.055350



cc Copyright © 2024 The Author(s). Published by Tech Science Press.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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