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Analyzing the Cytotoxic and Genetic Impact of Datura stramonium Extract on MCF7 and HT29 Cancer Cells: A Metabolite and Gene Expression Study

by Fayez Althobaiti1, Hadeer Darwish1,*, Raghad Alruqayb1, Saqer S. Alotaibi1, Fahad E. Alharthi2, Ibrahim Jafri1, Sarah Awwadh Altalhi1, Ghadi Alsharif3,4, Hussam Awwadh E Althagafi5, Hesham Noureldeen6, Sarah Alharthi7, Jawaher Albaqami2, Najla Amin T. Al Kashgry2, Ahmed Noureldeen2

1 Department of Biotechnology, College of Science, Taif University, Taif, 21944, Saudi Arabia
2 Department of Biology, College of Science, Taif University, Taif, 21944, Saudi Arabia
3 Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud Bin Abdulaziz University for Health Sciences, Jeddah, 21423, Saudi Arabia
4 Department of Biomedical Research, King Abdullah International Medical Research Center, Jeddah, 21423, Saudi Arabia
5 Biology Department, Faculty of Science and Arts, Al-Baha University, Al-Mikhwah, 65731, Saudi Arebia
6 Faculty of Medicine, Mansoura University, Mansoura, 35516, Egypt
7 Department of Chemistry, Collage of Science, Taif University, Taif, 21944, Saudi Arabia

* Corresponding Author: Hadeer Darwish. Email: email

(This article belongs to the Special Issue: Phytochemical and Pharmacological Research in Medicinal Plants)

Phyton-International Journal of Experimental Botany 2025, 94(1), 181-198. https://doi.org/10.32604/phyton.2025.059387

Abstract

The interest in using the Datura stramonium plant is due to its natural products, which are used in many pharmaceutical industries. The objective of the current study was to assess the therapeutic and cytotoxic effects of the D. stramonium plant on two types of human cancer cell models (MCF7 and HT29) in vitro. A soxhlet apparatus was used to obtain methanolic extract from dried plant leaves. The recovered crude, after the solvent had evaporated, was then dispersed at varied concentrations of extract 100, 50, 20, and 0.0 µg/mL and tested to see how the cells responded. Also, the cancer-testis antigen (CTA) gene transcription in the two cell types exposed to the plant extract was examined using a semi-quantitative real-time polymerase chain reaction. Gas chromatography–mass spectrometry (GC-MS) results produced the significant main metabolites Nonanoic acid, Tropine N-Oxide, 3,6-Ditigloyloxy-7-hydroxytropane, Hexadecanoic acid, 2-Pentadecanone,6,10,14-trimethyl-, Carvenone, methyl ester, Phytol, Aposcopolamine, Hyoscyamine, 4,8,12,16-Tetramethylheptadecan-4-olide, Scopolamine, Alpha.-Tocospiro A, 1,2-Cycloheptanedione, 3,3,7,7-tetramethyl-, dihydrazone, Campesterol, Stigmasterol, Gamma -Sitosterol and dl-.alpha.-Tocopherol. The results showed that the two types of cell lines impacted by D. stramonium extract, through untreated type 1 cells (MCF7) gave a highly significant transcription according to all applicable genes. All implemented analyses cleared the strong genetic impacts of Datura extract on cancer cells’ genomes. TGIF2LY and C2orf63 transcript accumulation were also significantly elevated when exposed to plant extract at a level of 50 µg/mL in cell line type 2 (HT29), but TGIF2LY and P53 had the lowest relative expression at a level of 100 µg/mL when treated the same cell line type.

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APA Style
Althobaiti, F., Darwish, H., Alruqayb, R., Alotaibi, S.S., Alharthi, F.E. et al. (2025). Analyzing the cytotoxic and genetic impact of datura stramonium extract on MCF7 and HT29 cancer cells: A metabolite and gene expression study. Phyton-International Journal of Experimental Botany, 94(1), 181–198. https://doi.org/10.32604/phyton.2025.059387
Vancouver Style
Althobaiti F, Darwish H, Alruqayb R, Alotaibi SS, Alharthi FE, Jafri I, et al. Analyzing the cytotoxic and genetic impact of datura stramonium extract on MCF7 and HT29 cancer cells: A metabolite and gene expression study. Phyton-Int J Exp Bot. 2025;94(1):181–198. https://doi.org/10.32604/phyton.2025.059387
IEEE Style
F. Althobaiti et al., “Analyzing the Cytotoxic and Genetic Impact of Datura stramonium Extract on MCF7 and HT29 Cancer Cells: A Metabolite and Gene Expression Study,” Phyton-Int. J. Exp. Bot., vol. 94, no. 1, pp. 181–198, 2025. https://doi.org/10.32604/phyton.2025.059387



cc Copyright © 2025 The Author(s). Published by Tech Science Press.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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