Open Access

ARTICLE

Genetic Diversity of Tuberose (Polianthes tuberosa L.) Germplasm through Molecular Approaches to Obtain Desirable Plant Materials for Future Breeding Programs

Vardhelli Soukya¹, Soumen Maitra^1,*, Nandita Sahana², Saikat Das³, Rupsanatan Mandal³, Arpita Mandal Khan¹, Arindam Das⁴, Ashok Choudhury⁵, Prodyut Kumar Paul⁶, Ahmed Gaber⁷, Mohammed M. Althaqafi⁸, Akbar Hossain^9,*

1 Department of Floriculture, Medicinal and Aromatic Plants, Faculty of Horticulture, Uttar Banga Krishi Viswavidyalya, Pundibari, Cooch Behar, 736165, West Bengal, India
2 Department of Biochemistry, Faculty of Agriculture, Uttar Banga Krishi Viswavidyalya, Pundibari, Cooch Behar, 736165, West Bengal, India
3 Department of Genetics and Plant Breeding, Faculty of Agriculture, Uttar Banga Krishi Viswavidyalya, Pundibari, Cooch Behar, 736165, West Bengal, India
4 Department of Vegetable and Spice Crops, Faculty of Horticulture, Uttar Banga Krishi Viswavidyalya, Pundibari, Cooch Behar, 736165, West Bengal, India
5 Department of Soil Science and Agricultural Chemistry, Faculty of Agriculture, Uttar Banga Krishi Viswavidyalya, Pundibari, Cooch Behar, 736165, West Bengal, India
6 Department of Pomology and Post-Harvest Technology, Faculty of Horticulture, Uttar Banga Krishi Viswavidyalya, Pundibari, Cooch Behar, 736165, West Bengal, India
7 Department of Biology, Faculty of Science, Taif University, P.O. Box 11099, Taif, 21944, Saudi Arabia
8 Department of Biotechnology, College of Sciences, Taif University, P.O. Box 11099, Taif, 21944, Saudi Arabia
9 Soil Science Division, Bangladesh Wheat and Maize Research Institute, Dinajpur, 5200, Bangladesh

* Corresponding Authors: Soumen Maitra. Email: ; Akbar Hossain. Email:

(This article belongs to the Special Issue: Advances in Plant Breeding and Genetic Improvement: Leveraging Molecular Markers and Novel Genetic Strategies)

Phyton-International Journal of Experimental Botany 2025, 94(11), 3493-3508. https://doi.org/10.32604/phyton.2025.071450

Received 05 August 2025; Accepted 10 October 2025; Issue published 01 December 2025

Abstract

The present study investigated the genetic diversity of 24 germplasms of Polianthes tuberosa L. via 16 inter simple sequence repeat (ISSR) marker techniques. The research findings revealed that the ISSR markers presented higher levels of band reproducibility and were more efficient at clustering germplasms. Among the 16 markers examined in this study, 12 had a complete polymorphism rate of 100%. The molecular analysis revealed a PIC ranging from 0.079 to 0.373, with a mean value of 0.30, whereas the range of the marker index was from 0.0001 to 0.409, with an average value of 0.03, and the primer resolving power ranged from 0.173 to 4.173, with a mean value of 2.02. The UPGMA clustering dendrogram indicated that all 24 germplasms were grouped into three main clusters. The study revealed a variable range of tree distances between 0.185 and 0.621, with the highest tree distance (0.621) detected between germplasms BR-24 and BR-1. Through these studies, the dissimilarity among the germplasms was evaluated, and diverse parents were identified for further crop improvement programs.

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Keywords

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