Sy-Yu Shiau^*, Shuting Ni, Yanli Yu, Songling Cai, Wenbo Huang

Phyton-International Journal of Experimental Botany, Vol.94, No.10, pp. 3047-3060, 2025, DOI:10.32604/phyton.2025.070289 - 29 October 2025

Abstract Consumers are increasingly demanding natural colorants that are safe and offer health benefits. In addition to their ornamental characteristics, Kanzan cherry (KC) blossoms present a promising source of red-hued natural colorants and functional bioactive substances. This research utilized distilled water to extract KC petals (KCP) and their ground powders (KCPP) under varying temperatures (30°C–90°C) and times (30–180 min). The total monomeric anthocyanins (TMAC) and total phenolics (TPC) in the extracts were evaluated via the pH differential and Folin–Ciocalteu methods. Antioxidant capacities were assessed by DPPH free radical scavenging ability and reducing power. Results indicated that… More >

Giovana Pinheiro Viana da Silva¹, Edilson Costa^1,*, Paulo Henrique Rosa Melo¹, Fernanda Pacheco de Almeida Prado Bortolheiro¹, Thaise Dantas², Flávio Ferreira da Silva Binotti¹, Carlos Eduardo da Silva Oliveira¹, Abimael Gomes da Silva¹

Phyton-International Journal of Experimental Botany, Vol.94, No.10, pp. 3109-3123, 2025, DOI:10.32604/phyton.2025.070221 - 29 October 2025

Abstract Hancornia speciosa ‘Gomes’, commonly known as mangabeira, is a fruit-bearing tree native to Brazil that plays a crucial role in sustaining its native biome, restoring degraded areas, and improving the socio-environmental conditions of these regions. The use of colored materials and bioinputs can help improve the quality of seedling production of Hancornia speciosa. This study aimed to evaluate the use of colored seedling tubes and a Chlorella vulgaris-based bioinput in developing Hancornia speciosa seedlings. The experiment was conducted at the Mato Grosso do Sul State University (UEMS), in Cassilândia, MS, using a completely randomized design in a 5 ×… More >

Heng Zhang^1,#, Meng Wang^1,#, Dong Zeng^1,2, Yunbo Xu¹, Dongyuan Guo¹, Xuanchen Liu^1,3, Zhanqi Ren⁴, Jinzi Zhang¹, Yuhang Liu¹, Qiuyu Wang¹, Shuo Yu¹, Guanzheng Qu^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.10, pp. 3205-3216, 2025, DOI:10.32604/phyton.2025.069994 - 29 October 2025

Abstract Hybridization remains an important method for breeding new poplar varieties. It results in significant variation in leaf phenotype among parents and offspring, and among offspring themselves. This study aimed to investigate whether leaf shape variations were similar in offspring produced from reciprocal crosses. Specifically, two hybrid combinations were produced: the direct cross with Populus ussuriensis as the maternal parent and P. simonii × P. nigra as the paternal parent (HY53), and the reciprocal cross with P. simonii × P. nigra as the maternal parent and P. ussuriensis as the paternal parent (HY268). Using 3-month-old rooted cuttings from 40 clones (36 F₁ hybrids… More >

Fida Hussain^1,*, Farooq Khan², Javed Ahmad¹, Heqiang Huo³, Tao Jiang³, Iqrar Rana⁴, Sajida Habib⁵, Muhammad Umer Farooq^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.10, pp. 3231-3249, 2025, DOI:10.32604/phyton.2025.069654 - 29 October 2025

Abstract The analysis of combining ability and heterosis is very important in enhancing the yield and oil quality of sunflowers under adverse conditions, and it reveals the potential of the parents and the mechanism of gene action. In this study, twenty-one hybrids were developed by crossing seven cytoplasmic male sterile (CMS) lines with three restorer lines and evaluated for agronomic and quality traits. Highly significant general combining ability (GCA) and specific combining ability (SCA) effects were observed, confirming the role of both additive and non-additive gene actions. Among the tested crosses, A-42 × R-86, A-92 ×… More >

Abdil Hakan Eren^*

Phyton-International Journal of Experimental Botany, Vol.94, No.10, pp. 3085-3107, 2025, DOI:10.32604/phyton.2025.069506 - 29 October 2025

Abstract The FAR1-related sequence (FAR1) gene family consists of transcription factors that originated from transposases and is crucial for light signaling and stress adaptation in plants. Despite the recognized importance of FAR1 genes in model organisms, their genomic architecture, structural variability, and expression patterns in Phaseolus vulgaris have yet to be investigated. This study offers the inaugural comprehensive genome-wide identification and characterization of the FAR1 gene family in P. vulgaris. A total of 27 PvulFAR1 genes were identified, and their chromosomal distribution, gene structures, conserved domains, and phylogenetic relationships were analyzed systematically. The promoter regions of these genes were discovered… More >

Yi Yan^1,2,#, Min He^1,2,#, Feifeng Mao³, Xinyu Zhang⁴, Liyu Wang⁵, Jingwei Li^1,2,*

Phyton-International Journal of Experimental Botany, Vol.94, No.10, pp. 3003-3021, 2025, DOI:10.32604/phyton.2025.069166 - 29 October 2025

Abstract Houttuynia cordata, a characteristic edible and medicinal plant in southwestern China, is prone to absorbing lead (Pb²⁺). Excessive consumption may lead to Pb²⁺ accumulation in the human body, which has been linked to serious health risks such as neurotoxicity, kidney damage, anemia, and developmental disorders, particularly in children. Therefore, the development of molecular markers associated with Pb²⁺ uptake and the investigation of the plant’s physiological responses to Pb²⁺ pollution are of great significance. In this study, 72 H. cordata germplasms were evaluated for Pb²⁺ accumulation after exogenous Pb²⁺ treatment. A significant variation in Pb²⁺ content was observed among the germplasms, indicating… More >

Paige M. Henning^*

Phyton-International Journal of Experimental Botany, Vol.94, No.10, pp. 2957-2983, 2025, DOI:10.32604/phyton.2025.068899 - 29 October 2025

Abstract Cytokinins are ancient hormones present across all kingdoms of life except archaea, although functional biosynthesis pathways have yet to be identified in animalia. Known for their roles in cell division and proliferation, cytokinins are critical to plant life, as they regulate various aspects of vegetative growth, stress response, and reproduction. In this review, we summarize literature from 2020 to 2025 pertaining to the cytokinin functions in plant reproduction. While general aspects of cytokinin’s role in plant reproduction have been addressed, we particularly focus on the role of cytokinin in reproductive systems due to recent work More >

Heba A. S. El-Nashar^1,*, Ahmed T. Negmeldin^2,3,*, Aziza El Baz⁴, Marizé Cuyler⁵, Brandon Alston⁵, Namrita Lall^5,6,7, Naglaa S. Ashmawy^1,8,*

Phyton-International Journal of Experimental Botany, Vol.94, No.10, pp. 3269-3281, 2025, DOI:10.32604/phyton.2025.067998 - 29 October 2025

Abstract Pimenta dioica is a tropical Caribbean tree belonging to the family Myrtaceae, widely used in various human activities, including perfume production, food flavoring, natural pesticides, and medicine. This study aimed to explore the chemical composition of Pimenta dioica flower essential oil obtained via hydrodistillation using GC-MS analysis. Additionally, the oil’s tyrosinase inhibitory activity was investigated. The effectiveness of the oil’s major constituents in binding to tyrosinase was also evaluated through molecular docking simulations. GC-MS analysis identified fifteen compounds, with eugenol (70.59%) as the major component, followed by β-myrcene (10.54%), limonene (8.55%), β-ocimene (4.92%), α-phellandrene (1.39%), and linalool… More >

Guiling Xu^1,#, Xiaoxuan You^1,#, Yuehua Feng^1,2,*, Xiaoke Wang¹, Yuqi Gao¹, Hongjun Ren¹, Zhili Han¹, Jiale Li¹

Phyton-International Journal of Experimental Botany, Vol.94, No.10, pp. 3251-3268, 2025, DOI:10.32604/phyton.2025.067997 - 29 October 2025

Abstract Nitrogen (N) reduction combined with organic fertilizer has become a highly popular fertilization method, meeting the sustainable development of agriculture. A field experiment was conducted to investigate the effects of N reduction (NR) and combined application of organic fertilizer (OF) on N utilization, yield, and quality of hybrid indica rice in the karst area. Using rice ‘Yixiangyou2115’ as the material, a split-plot design experiment was carried out with OF application rate as the main plots and NR rate as the subplots. The OF application rate had three levels: M0 (0 kg/ha), M1 (low OF, 1673… More >

Ahmed Mahmoud Ismail^1,*, Eman Said Elshewy², Ayman Y. Ahmed³, Hossam M. Darrag⁴

Phyton-International Journal of Experimental Botany, Vol.94, No.10, pp. 3023-3045, 2025, DOI:10.32604/phyton.2025.067444 - 29 October 2025

Abstract Powdery mildew of cucumber (Cucumis sativus L.) is a destructive disease caused by Podosphaera xanthii (Castagne) U.Braun & Shishkoff. This study aimed to investigate the antifungal effect of extracts of Ulva lactuca, Spirulina platensis, and Nostoc muscorum against P. xanthii and to improve the physiological and morphological traits of cucumber under commercial greenhouse conditions. The chemical composition of the individual extracts from U. lactuca, S. platensis, and N. muscorum was analyzed utilizing High-performance Liquid Chromatography (HPLC) and Gas Chromatography/Mass spectrometry (GC/MS). Cucumber plants were sprayed twice with 5% of the crude extracts of U. lactuca, S. platensis, and N. muscorum and their mixture (U. lactuca, S. platensis, and N. muscorum).… More >