Sushuang Liu^1,2,#, Yanmin Liu^1,2,#, Chundong Liu¹, Xingwang Yu^2,3, Hao Ma^2,4,*

Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 577-590, 2023, DOI:10.32604/phyton.2022.023738 - 12 October 2022

Abstract Drought is a major abiotic stress limiting agricultural crops production worldwide. In our study, we isolated a novel C2H2-type zinc finger protein gene ZF2 from chickpea. ZF2 consisted of 232 amino acids with two QALGGH motifs in Cys2/His2 zinc finger domain. Transient expression analysis of ZF2:GFP fusion protein showed that ZF2 was a nuclear localized protein. In the yeast assay system, the full-length of ZF2 did not show transcriptional activation. Expression of ZF2 gene was enhanced by treatments of several abiotic stresses and phytohormones. The promoter region of ZF2 contained multiple stress- and hormone-related cis-elements. Overexpression of More >

Lin Yuan¹, Jingcheng Zhang^2,*, Quan Deng², Yingying Dong³, Haolin Wang², Xiankun Du²

Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 611-628, 2023, DOI:10.32604/phyton.2022.023662 - 12 October 2022

Abstract Hyperspectral imaging technique is known as a promising non-destructive way for detecting plants diseases and pests. In most previous studies, the utilization of the whole spectrum or a large number of bands as well as the complexity of model structure severely hampers the application of the technique in practice. If a detection system can be established with a few bands and a relatively simple logic, it would be of great significance for application. This study established a method for identifying and discriminating three commonly occurring diseases and pests of wheat, i.e., powdery mildew, yellow rust… More >

Jiaxin Chen^1,#, Jiawei Guo^1,#, Zhixin Yang¹, Jiqing Yang^2,*, Hengwen Dong³, Huiyun Wang³, Yalei Wang³, Fangdong Zhan^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 471-485, 2023, DOI:10.32604/phyton.2022.023660 - 12 October 2022

Abstract Heavy metal-polluted soil was collected from the Pulang copper mine in Shangri-La City, Yunnan Province, Southwest China. The effects of fertilizer (organic and inorganic) and arbuscular mycorrhizal fungi (AMF) on ryegrass (Lolium multiflorum L.) growth, root morphology, mineral nutrition and cadmium (Cd) and lead (Pb) contents were investigated by pot experiments. The results showed that both fertilizer and AMF significantly ameliorated the root morphology and mineral nutrition, reduced the Cd and Pb contents, and promoted the growth of ryegrass. Among all treatments, the combined application of organic–inorganic compound fertilizer with AMF had the highest effect, resulting… More >

Sameh Barhoumi¹, Hasna Ellouzi², Abdelmajid Krouma^1,3,*

Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 521-536, 2023, DOI:10.32604/phyton.2022.023555 - 12 October 2022

Abstract Lime-induced iron chlorosis is a major nutritional disorder causing severe plant growth and yield reduction in the calcareous soils of Tunisia. The understanding the behavior of key metabolic functions of peas on calcareous soils, the identification of useful traits of tolerance, and the exploration of the genotypic differences in response to this constraint remain the most efficient approaches due to their coast, environmental benefits, and sustainability. For this purpose, a greenhouse experiment was conducted on three pea genotypes (Alexandra: Alex, Douce de provence: DP, and Merveille de Kelvedon: MK) cultivated on calcareous soil (Fe-deficient) and… More >

Liyan Wu¹, Jie Cheng^1,2, Yaju Gong¹, Rui Bao¹, Zhibin Li¹, Min Gui^1,*, Guanghui Du^2,*

Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 591-609, 2023, DOI:10.32604/phyton.2022.023481 - 12 October 2022

Abstract In this study, wild eggplant germplasm No. M239, which is highly susceptible to Verticillium wilt, was used as the experimental material. The physiological and biochemical indices (SOD, PAL, MDA and soluble protein) of M239 roots were measured at different times (0, 12, 24, 36, 48, 60 and 72 h) post inoculation with Verticillium dahliae, and the key time points for the M239 response to Verticillium wilt infection were screened. Then, RNA-Seq technology was used to screen the differentially expressed genes (DEGs) in M239 roots at 0, 12 and 48 h post-inoculation (hpi). The transcriptional results of M239… More >

Shuo Qu¹, Qi Cai², Huimin Cui¹, Lamboro Abraham¹, Yaolei Jiao¹, Guilong Ma², Piwu Wang^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 501-519, 2023, DOI:10.32604/phyton.2022.023479 - 12 October 2022

Abstract High yield, high quality, stable yield, adaptability to growth period, and modern mechanization are the basic requirements for crops in the 21st century. Soybean oleic acid is a natural unsaturated fatty acid with strong antioxidant properties and stability. Known as a safe fatty acid, it has the ability to successfully prevent cardiovascular and cerebrovascular disorders. Improving the fatty acid composition of soybean seeds, can not only speed up the breeding process of high-quality high-oil and high-oleic soybeans, but also have important significance in human health, and provide the possibility for the development of soybean oil… More >

Ruizhi Huang¹, Huasheng Yu², Yong Yang¹, Heqin Liu², Xuelong Wu¹, Zhihong Liu¹, Haiyan He¹, Gengwei Wu¹, Wengjia Wang¹, Hua Wang^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 333-348, 2023, DOI:10.32604/phyton.2022.023252 - 12 October 2022

Abstract As global temperature rise, the threat of heat stress to rapeseed production is becoming more obvious. Exploring the response characteristics of two important biological pathways, oil accumulation and photosynthesis, to heat stress during B. napus seed filling is helpful in the genetic improvement of heat-tolerant rapeseed. The effects of heat stress on seed oil accumulation and chlorophyll fluorescence characteristics of 29 B. napus germplasms with different oil content and environmental sensitivity, including 6 rapeseed varieties which exhibited environment-sensitive/insensitive and with high, medium or low oil content, were tested by whole plant heat stress or the in vitro silique… More >

Fang Dong¹, Qian Fan², Xinguo Su^3,*, Lanting Zeng^4,*

Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 383-398, 2023, DOI:10.32604/phyton.2022.023185 - 12 October 2022

Abstract Aroma (volatile) compounds play important ecological functions in plants, and also contribute to the quality of plant-derived foods. Moreover, chiral aroma compounds affect their functions in plants and lead to different flavor quality properties. Formations of chiral aroma compounds are due to the presence of enzymes producing these compounds in plants, which are generally involved in the final biosynthetic step of the aroma compounds. Here, we review recent progress in research on the plant-derived enzymes producing chiral aroma compounds, and their changes in response to environmental factors. The chiral aroma enzymes that have been reported More >

Wei Tian¹, Lingdi Liu¹, Zhongxing Song², Zhishu Tang², Chunxiu Wen¹, Tao Jiang^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 369-382, 2023, DOI:10.32604/phyton.2022.023184 - 12 October 2022

Abstract The extract from Semen Ziziphi Spinosae (SZS) contains many secondary metabolites (flavonoids, alkaloids, and terpenoids), widely reported for their high medicinal value. In order to know the number and type of flavonoids and the regulating mechanism of flavonoid biosynthesis during the growth and development of SZS, we identified and analyzed the flavonoid metabolites and genes in SZS using metabolomic and transcriptomic. A total of 13,232 differentially expressed genes and 83 flavonoid metabolites were identified at three different growth and development stages (T1, T2, and T3) of SZS. The main flavonoid metabolic components of SZS were catechin, More >

Danlei Song^#, Xiaona Yu^#, Yaoyao Li, Xianheng Wang, Xinyuan Cui, Tong Si, Xiaoxia Zou, Yuefu Wang, Minglun Wang, Xiaojun Zhang^*

Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 439-453, 2023, DOI:10.32604/phyton.2022.023165 - 12 October 2022

Abstract The high-quality genomes and large-scale full-length cDNA sequences of allotetraploid peanuts have been sequenced and released, which has accelerated the functional genomics and molecular breeding research of peanut. In order to understand the difference in the transcriptional levels of wild and cultivated peanuts. In this study, we integrated of second- and third-generation sequencing technologies to sequence full-length transcriptomes in peanut cv. Pingdu9616 and its putative ancestor Arachis monticola. The RNA extracted from six different tissues (i.e., roots, stems, leaves, flowers, needles and pods) were sampled at 20 days after flowering. A total of 31,764 and 33,981… More >