Deng JB^1,2*, G Gao³, KS Ahmad⁴, XQ Luo^1,2, FT Zhang^1,2, S Li^1,2, RW Yang⁵

Phyton-International Journal of Experimental Botany, Vol.87, pp. 156-161, 2018, DOI:10.32604/phyton.2018.87.156

Abstract Six mitochondrial (mtDNA) markers, cox2, ccmFn, ccb256, cox3, Nad4L-orf25, and atp4, were combined to assess genetic relationships by using Maximum Likelihood (ML) and Maximum parsimony (MP). This was done among nine Chinese Curcuma herbal species, included two rare species which are difficult to distinguish from their morphological characters. The relationships are very close among the nine species, and the overall distance was 0.018. In this study, the backbone of such nine species was achieved firstly to date, and being divided into two groups with moderate to strong bootstrap support. Both the ML and MP tree were More >

Xin ZM^1,2, MH Liu², Q Lu^1,3, CA Busso⁵, YJ Zhu^1,3, Z Li², YR Huang², XL Li2, FM Luo², F Bao¹, JQ Qian^4*, YH Li^1,3*

Phyton-International Journal of Experimental Botany, Vol.87, pp. 144-155, 2018, DOI:10.32604/phyton.2018.87.144

Abstract Leaf δ¹³C is widely used to explain plant strategies related to resource availability in different environments. However, the coupled response of leaf δ¹³C to precipitation and temperature as well as the relationship between leaf δ¹³C and leaf traits remain unclear. The leaf δ¹³C and its relationship with leaf traits [leaf size (LS), leaf length (LL), leaf width (LW), leaf length to width ratio (L:W), specific leaf area (SLA) and mass-based leaf nitrogen concentration (N_mass)] were investigated on the dominant shrub species Nitraria tangutorum Bobr. in the arid region (Dengkou and Minqin) of northwestern China under the simulated increasing precipitation… More >

Jabbari H¹, M Gholamhosseini¹, M Naeemi², A Nasiri³

Phyton-International Journal of Experimental Botany, Vol.87, pp. 133-142, 2018, DOI:10.32604/phyton.2018.87.133

Abstract Two experiments were performed in 2011-2012 to investigate the response of three oilseed rape cultivars to drought stress, grown under two climate conditions in Iran (cold and hot climates). The experiments were conducted using a randomized complete-block design arranged in split-plot with three replicates. The irrigation treatments (FI: full irrigation; WIF: withholding irrigation at flowering stage, and WIS: withholding irrigation at the silique formation stage until physiological maturity) were allocated to main plots, whereas subplots consisted of the oilseed rape cultivars: early maturating (GKH2005), relatively late maturing (Opera) and late maturing (Okapi). Drought caused a… More >

Wang XG¹, Wuyunna^1*, CA Busso², YT Song¹, FJ Zhang¹, GW Huo¹

Phyton-International Journal of Experimental Botany, Vol.87, pp. 123-132, 2018, DOI:10.32604/phyton.2018.87.123

Abstract Chemical elements, such as carbon (C), nitrogen (N) and phosphorus (P), are major limiting nutrients in arid and semiarid grasslands and their stoichiometry (C:N:P) is a very important ratio to determine. In addition, it is critical to understand how plant stoichiometry responds to multiple environmental factors at the species level. In this study, we conducted a greenhouse experiment to investigate the effects of salt stress (4 g NaCl/kg soil), drought (35% of the soil water holding capacity) and N addition (10 g N/m²), as well as their interactions, on C, N and P concentrations and C:N:P… More >

Han DY^1,2, YF Yang²

Phyton-International Journal of Experimental Botany, Vol.87, pp. 113-122, 2018, DOI:10.32604/phyton.2018.87.113

Abstract Rapid and precise assessment of biodiversity is a central issue in conservation biology and biodiversity science. Higher taxonomic units and life form-based morphospecies were utilised to assess plant diversity patterns at the habitat scale in temperate meadow steppe, Songnen Plain, China. Patch area, taxonomic composition (families and genera) and life form (based on Raunkiaer’s life forms, and stem and root growth forms) were recorded in five communities. Taxonomic unit-based hierarchical diversity indices (HDI) and life form-based hierarchical diversity indices (HLDI) were calculated. Twenty-six families, 67 genera, and 87 species were recorded in the five communities.… More >

Espinoza-Sánchez EA¹, YA Sánchez-Peña², JA Torres-Castillo³, EA García-Zambrano², JE Treviño Ramírez², F Zavala-García², SR Sinagawa-García²

Phyton-International Journal of Experimental Botany, Vol.87, pp. 105-112, 2018, DOI:10.32604/phyton.2018.87.105

Abstract In this study, we established an in vitro regeneration system from immature embryos of four varieties of Sweet Sorghum with biotechnological potential. A total of 17 treatments were assesses in the induction of somatic embryogenic calli. We analyzed the effect of Murashige y Skoog media supplemented with different concentrations of plant growth regulators such as auxins and cytokinins. The callus induction response was evaluated using three immature embryos development stages and based on the number of embryos with callus induction. Different responses were observed in the embryos depending of the embryo age, culture condition, media… More >

Jimarez-Montiel MJ¹, A Robledo-Paz¹, VM Ordaz-Chaparro², LI Trejo-Tellez², JC Molina-Moreno¹

Phyton-International Journal of Experimental Botany, Vol.87, pp. 94-104, 2018, DOI:10.32604/phyton.2018.87.094

Abstract An alternative method for improving the production of habanero chili is the tissue culture technique; however, the gelling agent, the high salt and sucrose concentrations used in the culture media raise production costs and limit the adaptation of the regenerated plants to greenhouse or field conditions. In this study, the effect of the substrates perlite-coconut fiber, coconut fiber-volcanic rock, vermiculite-perlite, and perlite-volcanic rock in conjunction with various culture media in in vitro plant regeneration from embryos was evaluated. The differentiation of adventitious shoots on substrates was scarcely observed or non-existent. Inducing the formation of shoots on… More >

García-Zambrano EA¹, MC Ojeda-Zacarías¹, JG Gutiérrez-Soto¹, SR Sinagawa-García¹, A Gutiérrez-Díez¹, J Martínez-de la Cerda¹, JA Pedroza-Flores¹, C Gallegos-Vázquez², JI García-Zambrano³

Phyton-International Journal of Experimental Botany, Vol.87, pp. 87-93, 2018, DOI:10.32604/phyton.2018.87.087

Abstract The technologies based on molecular markers of DNA are the most advanced and possibly the most effective and accurate means to identify genetic variation. The objective of this study was to use molecular genetic markers namely AFLP as a tool for the molecular differentiation of 36 accessions of xoconostle collected in the Centro Regional Universitario Centro Norte of the Universidad Autónoma de Chapingo (CRUCEN-UACh.). DNA extraction was performed by the method of cellular ruptor using FastDNA^® Kit, and quantification of purity and yield of DNA was carried out through the Nanodrop 2000. It was observed that… More >

Rodrigues Costa A^1,2, PS Pereira³, MK Alves de Sousa^1,2, FC Rodrigues⁵, VR Duarte Mendes¹, KR Rodrigues Lima¹, L Marivando Barros^1,4, CM Rocha Sousa¹, MS Alcântara¹, AB Leite Cavalcante⁴, R Cruz Macedo⁵, JP Kamdem³, AE Duarte^1,2

Phyton-International Journal of Experimental Botany, Vol.87, pp. 79-86, 2018, DOI:10.32604/phyton.2018.87.079

Abstract The species Rhaphiodon echinus, known as betonica or creeping mint, is considered an infesting plant species, typical of the caatinga biome. Morphoanatomy is a very important tool to study the structure of plants, both its external and internal morphology, opening the knowledge about the production of secondary metabolites. These compounds are of great importance for scientific research, which may present themselves as the best antioxidants. Taking into account a lack of data in the literature on the essential oil of R. echinus, this study aimed to demonstrate its antioxidant activity and toxicity. In order to test this… More >

Villa-Hernández JM¹, B García-Ocón², E del C Sierra-Palacios², C Pelayo-Zaldivar³, F Díaz de León-Sánchez¹, LJ Pérez-Flores¹, JA Mendoza-Espinoza²

Phyton-International Journal of Experimental Botany, Vol.87, pp. 72-78, 2018, DOI:10.32604/phyton.2018.87.072

Abstract Mexico bears witness to a long-standing tradition in the use of plants for medicinal purposes; nevertheless, standardized methodological approaches for the proper identification of these are still needed. The problem is especially noticeable during the validation of ingredients in herbal preparations, as so many of them are sold as powders. It is for these reasons that the present study aims to compare the results of classic taxonomical assessments with those obtained using more modern molecular techniques (e.g. PCR-amplified 18S ribosomal RNA gene analysis) in one ethnobotanical case-study carried out in the “Desierto de los Leones” National… More >