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  • Open Access

    ARTICLE

    Hypoglycemic Lignans from Amomum tsao-ko Leaves: Their α-Glucosidase Inhibitory Mechanism Integrated In Silico and In Vivo Validation

    Yun Wang1,2,#, Xin-Yu Li1,3,#, Sheng-Li Wu1,3, Pianchou Gongpan1, Da-Hong Li2, Chang-An Geng1,3,*

    Phyton-International Journal of Experimental Botany, Vol.94, No.8, pp. 2563-2574, 2025, DOI:10.32604/phyton.2025.068185 - 29 August 2025

    Abstract Twelve lignans (1–12) isolated from Amomum tsao-ko leaves were evaluated for the inhibitory effects against α-glucosidase and PTP1B. Compounds 1−4 and 10 showed inhibition on α-glucosidase with inhibitory ratios ranging from 53.8% to 90.0%, while compound 10 demonstrated 56.1% inhibition on PTP1B at 200 μM. Notably, erythro-5-methoxy-dadahol A (2) and threo-5-methoxy-dadahol A (3) displayed obvious inhibition on α-glucosidase with IC50 values of 33.3 μM and 22.1 μM, significantly outperforming acarbose (IC50 = 344.0 μM). Kinetic study revealed that compound 3 maintained a mixed-type mode, engaging with both free enzyme and enzyme-substrate complex via non-competitive and uncompetitive mechanisms. Molecular docking simulations further clarified its More >

  • Open Access

    ARTICLE

    Chemical Constituents from Turnip and Their Effects on α-Glucosidase

    Swastika Paul1,3, Xuemei Zhang1, Yongping Yang2, Changan Geng1,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 131-136, 2020, DOI:10.32604/phyton.2020.08328 - 01 March 2020

    Abstract Brassica rapa var. rapa (turnip) is an important crop in Qinghai-Tibet Plateau (QTP) with anti-hypoxic effect. Turnip is rich in glucosinolates, isothiocyanates and phenolic compounds with diverse biological activities, involving anti-oxidant, anti-tumor, anti-diabetic, anti-inflammatory, anti-microbial, hypolipidemic, cardioprotective, hepatoprotective, nephroprotective and analgesic properties. In this study, the ethyl acetate (EtOAc) and butanol parts of Brassica rapa were first revealed with inhibitory effects on α-glucosidase, whereas the water part was inactive. Subsequent bioassay-guided isolation on the EtOAc and butanol parts yielded 12 compounds, involving three indole derivatives, indole-3- acetonitrile (1) 4-methoxyindole-3-acetonitrile (2) and indole-3-aldehyde (3) two flavonoids, liquiritin (4) and… More >

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