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  • Open Access


    Overexpression of β-1,4-Glucanase Gene EuEG1 Improves Micrografting of Eucommia ulmoides

    Lei Wang1, Runying Wang1, Yi Li3, Yichen Zhao1,*, Degang Zhao1,2,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 3063-3075, 2023, DOI:10.32604/phyton.2023.043803

    Abstract Adventitious root formation poses a major constraint on the tissue culture and genetic transformation of Eucommia ulmoides. Micrografting offers a new method for the transplantation of genetic transformation, and its success depends on the formation of graft unions. This study used transgenic rootless test-tube seedlings as scions and seedlings from seed as rootstocks during micrografting to avoid the rooting issues that occur during tissue culture and to investigate the role of the EuEG1 gene in the graft healing process. We found that the EuEG1 gene is a vital regulator of graft, and its overexpression contributes to the… More >

  • Open Access


    Ectopic Overexpression of EuCHIT30.7 Improves Nicotiana tabacum Resistance to Powdery Mildew

    Lanjun Li1, Degang Zhao1,2,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 3043-3061, 2023, DOI:10.32604/phyton.2023.031175

    Abstract Various strains of powdery mildew (PM), a notorious plant fungal disease, are prevalent and pose a significant threat to plant health. To control PM, transgenic technology can be used to cultivate more resistant plant varieties. In the present study, we utilized the rapid amplification of cDNA ends (RACE) technique to clone the full-length cDNA sequence of the EuCHIT30.7 gene to explore plant genes with disease resistance functions. Bioinformatics analysis revealed that this gene belongs to the GH18 family and is classified as a class III chitinase. The EuCHIT30.7 gene is expressed throughout the Eucommia ulmoides plant, with… More >

  • Open Access


    Comprehensive Assessment of the Safety of Eucommia ulmoides Leaf Extract for Consumption as a Traditional Chinese Health Food

    Huiling Fu1, Mijun Peng2, Qiuwen Tang2, Haojun Liang2, Yanli Liang2, Jiali Fang2,*, Xuesong Wang2,*

    Journal of Renewable Materials, Vol.11, No.7, pp. 3091-3114, 2023, DOI:10.32604/jrm.2023.026689

    Abstract To ensure the export quality of Eucommia ulmoides leaf extract (ELE) and facilitate E. ulmoides leaf inclusion in the directory of traditional Chinese health foods, an overall safety assessment of ELE was performed, including genotoxicity and long-term toxicity, according to the national food safety standards of China. No variations in the reverse mutation number of the nominal bacterial strains were observed under ELE treatment in comparison with the solvent control. Additionally, the micronucleus rates of in vivo mammalian erythrocytes and in vitro mammalian cells under ELE treatment were equivalent to or significantly lower than those of the… More > Graphic Abstract

    Comprehensive Assessment of the Safety of <i>Eucommia ulmoides</i> Leaf Extract for Consumption as a Traditional Chinese Health Food

  • Open Access


    Cloning and Function Identification of a Phytoene Desaturase Gene from Eucommia ulmoides

    Jiali Wang1, Xiangmei Chen1, Xiaozhen Huang1, Yichen Zhao1,*, Degang Zhao1,2,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.5, pp. 1377-1389, 2023, DOI:10.32604/phyton.2023.026830

    Abstract The phytoene desaturase (PDS) encodes a crucial enzyme in the carotenoid biosynthesis pathway. Silencing or inhibiting PDS expression leads to the appearance of mottled, chlorosis, or albino leaves. In this study, the CDS sequence of EuPDS (Eucommia ulmoides Phytoene Desaturase) was first cloned and then PDS was silenced in Nicotiana benthamiana. Result showed the expression level of EuPDS in leaves was higher than that in the roots and stems. In N. benthamiana leaves, which were treated by Agrobacterium for 24 h, photo-bleaching was shown on the fresh leaves one week after injection and the transcript level of PDS was down-regulated during the More >

  • Open Access


    Determining the in vitro Anti-Aging Effect of the Characteristic Components from Eucommia ulmoides

    Xuesong Wang1,#, Zhihong Wang1,2,#, Qiuling Yang1, Sheng Peng3, Mijun Peng1,*

    Journal of Renewable Materials, Vol.10, No.12, pp. 3131-3145, 2022, DOI:10.32604/jrm.2022.020204

    Abstract To evaluate the potential anti-aging ability of Eucommia ulmoides, four characteristic components (chlorogenic acid, geniposidic acid, aucubin, quercetin) were selected to assess their effects on H2O2-induced oxidative damage model of human umbilical vein endothelial cell (HUVEC). Oxidative damage indexes, inflammatory factors, cell cycle, cell apoptosis, cell senescence, and their related proteins were analyzed by methyl thiazolyl tetrazolium (MTT) assay, enzyme-linked immunosorbent assay (ELISA), propidium iodide (PI) staining, annexin V-FITC/ PI double staining, SA β-galactosidase staining, and western blotting (WB). The results showed that H2O2- induced cell growth inhibition rate decreased as supplementation with characteristic components when… More >

  • Open Access


    Transcriptomic and Physiological Analyses of Pigment Accumulation in Eucommia ulmoides ‘Hongye’

    Mengjiao Chen1,2, Jinhui Zhai3, Jiajia Zhang1,4,5,6, Hui Li1,2, Xinjiang Niu1,2, Yaxin Liu1,2, Yue Ren1,2, Hongyan Du1,4,5,6, Jingle Zhu1,4,5,6,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.5, pp. 1027-1044, 2022, DOI:10.32604/phyton.2022.019106

    Abstract Eucommia ulmoides ‘Hongye’ is a new ornamental variety of E. ulmoides with excellent red or purple foliage. We found that E. ulmoides ‘Hongye’ exhibited a gradual change from green to red colour under light conditions. However, the colouring mechanism in the leaves of E. ulmoides ‘Hongye’ remains unclear. In this study, we compared the pigment content and leaf colour index of E. ulmoides ‘Hongye’ at five stages with those of E. ulmoides ‘Xiaoye’, which was used as the control variety. The transcriptome sequencing data of the first-period (H1, green) and fifth-period (H5, red) leaves were also analysed and compared. The corresponding… More >

  • Open Access


    Cloning and Characterization of EuGID1 in Eucommia ulmoides Oliver

    Yulu Chen1, Fen Zhang1, Ju Cai1, Yichen Zhao1,2, Jiaxue Cui1, Yan Li1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.5, pp. 999-1013, 2022, DOI:10.32604/phyton.2022.018984

    Abstract Gibberellic acid controlled the key developmental processes of the life cycle of landing plants, and regulated the growth and development of plants. In this study, a novel gibberellin receptor gene EuGID1 was obtained from Eucommia ulmoides Oliver. The cDNA of EuGID1 was 1556 bp, and the open reading frame was 1029 bp, which encoded 343 amino acids. EuGID1 had the homology sequence with the hormone-sensitive lipase family. Amino acid sequence alignment confirmed EuGID1 protein had the highest homology with the GID1 protein of Manihot esculenta. EuGID1 was located in the nucleus and cell membrane and had expression in four plant More >

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