Yongjing Xi, Zhou Yang, Yukun Jin, Jing Qu, Shuyan Guan, Siyan Liu, Piwu Wang*
Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1257-1274, 2023, DOI:10.32604/phyton.2023.025918
Abstract PCR detection, quantitative real-time PCR (q-RTPCR), outdoor insect resistance, and disease resistance identification were carried out for the detection of genetic stability and disease resistance through generations (T2, T3, and T4) in transgenic maize germplasms (S3002 and 349) containing the bivalent genes (insect resistance gene Cry1Ab13-1 and disease resistance gene NPR1) and their corresponding wild type. Results indicated that the target genes Cry1Ab13-1 and NPR1 were successfully transferred into both germplasms through tested generations; q-PCR confirmed the expression of Cry1Ab13-1 and NPR1 genes in roots, stems, and leaves of tested maize plants. In addition, S3002 and 349 bivalent gene-transformed lines… More >
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