Open Access

ARTICLE

Identification and Evaluation of Insect and Disease Resistance in Transgenic Cry1Ab13-1 and NPR1 Maize

Yongjing Xi, Zhou Yang, Yukun Jin, Jing Qu, Shuyan Guan, Siyan Liu, Piwu Wang^*

Joint Laboratory of International Cooperation in Modern Agriculture Technology of Ministry of Education, Plant Biotechnology Center, Jilin Agricultural University, Changchun, China

* Corresponding Author: Piwu Wang. Email:

(This article belongs to this Special Issue: Abiotic and Biotic Stress Tolerance in Crop)

Phyton-International Journal of Experimental Botany 2023, 92(4), 1257-1274. https://doi.org/10.32604/phyton.2023.025918

Received 05 August 2022; Accepted 21 October 2022; Issue published 06 January 2023

Abstract

PCR detection, quantitative real-time PCR (q-RTPCR), outdoor insect resistance, and disease resistance identification were carried out for the detection of genetic stability and disease resistance through generations (T₂, T₃, and T₄) in transgenic maize germplasms (S3002 and 349) containing the bivalent genes (insect resistance gene Cry1Ab13-1 and disease resistance gene NPR1) and their corresponding wild type. Results indicated that the target genes Cry1Ab13-1 and NPR1 were successfully transferred into both germplasms through tested generations; q-PCR confirmed the expression of Cry1Ab13-1 and NPR1 genes in roots, stems, and leaves of tested maize plants. In addition, S3002 and 349 bivalent gene-transformed lines exhibited resistance to large leaf spots and corn borer in the field evaluation compared to the wild type. Our study confirmed that Cry1Ab13-1 and NPR1 bivalent genes enhanced the resistance against maize borer and large leaf spot disease and can stably inherit. These findings could be exploited for improving other cultivated maize varieties.

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