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  • Open Access

    ARTICLE

    Suspension Culture and Somatic Embryogenesis of Korean Pine

    Chunxue Peng1,2, Fang Gao1,2, Hao Wang1,2, Iraida Nikolaevna Tretyakova3, Alexander Mikhaylovich Nosov4,5, Hailong Shen1,2,*, Ling Yang1,2,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.1, pp. 223-238, 2022, DOI:10.32604/phyton.2022.015523

    Abstract Korean pine is an important afforestation tree species in Northeast China, which has a high ecological and economic value. Although regeneration of somatic embryogenesis using immature zygotic embryos of Korean pine as explants has been successful, it cannot be applied to automation and large-scale production. Therefore, we urgently need a method that can increase the output of somatic embryos (SEs) to meet the needs of large-scale production. We used Korean pine 1–1 and 1–100 cell lines as research materials to evaluate the effects of inoculum-density, culture time, orbiting speed, vessel volume, plant growth regulator (PGR) concentration, and carbon source on… More >

  • Open Access

    ARTICLE

    Genetic Variation in Growth and Cone Traits of Pinus Koraiensis Half-Sib Families in Northeast China

    David Kombi Kaviriri1,3,4,#, Xiaoting Liu1,#, Zuoyi Fan2,#, Jingyuan Wang2, Qi Wang2, Lianfu Wang2, Lixing Wang2, Damase Khasa5, Xiyang Zhao1,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 57-69, 2020, DOI:10.32604/phyton.2020.08409

    Abstract Genetic parameters were evaluated for growth and cone characteristics (tree height, diameter at breast height, volume, cone number, thousand seeds weight and single cone seeds weight) on 86 half-sib families of Pinus koraiensis aged 31 years. Analyses of variance revealed significant differences (p < 0.001) in all growth and cone traits among families while no significant differences were detected among blocks and the interaction between blocks and families. The average family values for growth traits were 17.22 m, 8.67 cm and 0.43 m3 for tree height, diameter at breast height and volume, respectively. The average cone number, thousand seeds weight… More >

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