PENG LIU^1,2, ANFANG ZOU³, QI CHEN⁴, BIAO CHENG^1,*, QIN LI^1,*

BIOCELL, Vol.45, No.1, pp. 27-39, 2021, DOI:10.32604/biocell.2021.012601

Abstract The diabetic ulcer is one of the serious complications of diabetes. In this study, we aimed to establish an exosomal microRNA (miRNA)-targeted messenger RNA (mRNA) regulatory network for screening new biomarkers for diabetic ulcer treatment. For this purpose, exosomes were extracted from bone marrow stem cells (BMSCs) collected from diabetic ulcer patients and healthy adults. The miRNAs in exosomes was detected by high-throughput sequencing analysis. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the differential miRNAs were performed. The miRNA-mRNA regulatory network between candidate miRNAs and their target genes were constructed by… More >

Muyi Guo¹, Yan Cai¹, Zhiyong Li^1,2,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.1, pp. 49-49, 2019, DOI:10.32604/mcb.2019.05727

Abstract Observational studies have identified angiogenesis from the adventitial vasa vasorum and intraplaque hemorrhage (IPH) as critical factors in atherosclerotic plaque progression and destabilization. Here we propose a mathematical model incorporating intraplaque neovascularization and hemodynamic calculation for the quantitative evaluation of atherosclerotic plaque hemorrhage. An angiogenic microvasculature based on histology of a patient’s carotid plaque is generated by two-dimensional nine-point model of endothelial cell migration. Three key cells (endothelial cells, smooth muscle cells and macrophages) and three key chemicals (vascular endothelial growth factors, extracellular matrix and matrix metalloproteinase) are involved in the intraplaque angiogenesis model, and described by the reaction-diffusion partial… More >

A. Shukla^1,1, A.R. Dunn^2,2, M.A. Moses^3,3, K.J. Van Vliet^4,4

Molecular & Cellular Biomechanics, Vol.1, No.4, pp. 279-290, 2004, DOI:10.3970/mcb.2004.001.279

Abstract Although it is established that endothelial cells can respond to external mechanical cues (e.g., alignment in the direction of fluid shear stress), the extent to which mechanical stress and strain applied via the endothelial cell substrate impact biomolecular and cellular processes is not well-understood. This issue is particularly important in the context of inflammation, vascular remodeling, and cancer progression, as each of these processes occurs concurrently with localized increases in strain and marked changes in molecules secreted by adjacent cells. Here, we systematically vary the level and duration of cyclic tensile strain applied to human dermal microvascular and bovine capillary… More >

Chunhua YANG^1,2,3, Ye TIAN^1,2,3

BIOCELL, Vol.43, No.3, pp. 207-214, 2019, DOI:10.32604/biocell.2019.07258

Abstract Sperm-associated antigen 9 (SPAG9) expression is increased in prostate tissues of prostate cancer patients. This experimental study aimed to investigate the role of SPAG9 in bone metastasis of prostate cancer. Immunohistochemical analysis showed that SPAG9 staining was positive in 81.67% of 240 cases of prostatic carcinoma but only in 6.67% of 120 cases of benign prostate hyperplasia. Strong PAG9 staining was positively correlated with Gleason score and bone metastasis in 240 prostate cancer patients (p < 0.05), but not with the age or serum prostatespecific antigen level (p > 0.05). PC-3 cells were transfected with shRNA against SPAG9, and CCK-8… More >

Bo Ling^1,#, Daoxi Lei^1,#, Juhui Qiu¹, Kang Zhang¹, Hao Chen^2,*, Yeqi Wang¹, Zhiyi Ye¹, Guixue Wang^*

Molecular & Cellular Biomechanics, Vol.14, No.4, pp. 197-211, 2017, DOI:10.3970/mcb.2017.014.197

Abstract Shear stress and oxidized low-density lipoprotein (oxLDL) caused by abnormal blood is critical to angiogenesis for atherosclerosis. However, the mechanism in shear stress or ox-LDL regulated angiogenesis is still not well understood. There is the hypothesis that shear stress or oxLDL regulates angiogenesis through the vascular endothelial growth factor (VEGF) signaling pathway. It is discovered that both high shear stress and low concentration of oxLDL contribute to angiogenesis, which is inhibited once the VEGF or VEGFR expression is knocked down. The expression of p-FAK and p-paxillin is regulated by the VEGF/VEGFR signal axis. VEGFR2, p-FAK, p-paxillin and VEGFR1 are VEGF-responsive… More >

Yidan Chen^#,1, Kang Zhang^#,1, Juhui Qiu¹, Shicheng He¹, Junyang Huang², Lu Huang¹, Dongyu Jia³, Bo Ling¹, Da Sun⁴, Xiang Xie¹, Tieying Yin^*,1, Guixue Wang^*,1

Molecular & Cellular Biomechanics, Vol.14, No.2, pp. 83-100, 2017, DOI:10.3970/mcb.2017.014.081

Abstract Abnormal shear stress in the blood vessel is an important stimulating factor for the formation of angiogenesis and vulnerable plaques. This paper intended to explore the role of shear stress-regulated Id1 in angiogenesis. First, we applied a carotid artery ring ligation to create local stenosis in ApoE^-/- mice. Then, 3D geometry of the vessel network was reconstructed based on MRI, and our analysis of computational fluid dynamics revealed that wall shear stress of the proximal region was much higher than that of the distal region. In addition, results from histological staining of the proximal region found more vulnerable-probe plaques with… More >

Jie Wu^∗,†, Quan Long^‡, Shixiong Xu^*, Anwar R. Padhani^§, Yuping Jiang^¶

Molecular & Cellular Biomechanics, Vol.5, No.4, pp. 217-228, 2008, DOI:10.3970/mcb.2008.005.217

Abstract In this paper, a 3D mathematical model of tumour angiogenesis is developed, to generate a functional tumour vasculature for blood microcirculation. The model follows that of Anderson and Chaplain (1998) ^[1] with three exceptions: (a) extending the model from 2D to 3D, one arteriole and one venule is induced as two parent vessels to form an intact circulation network for blood flow; (b) generating networks able to penetrate into the tumour interior rather than the exterior only; (c) considering branching generations with different diameters, based on which three groups of vessels, such as arterioles, venules and capillaries are classified. The… More >