XIRU XU1,#, YUAN FANG1,#, BIAO ZHANG1,*, SHICHAO TENG1, XIANG WU1, JING ZHANG1, XIAOQUN GU2, MEIXIA MA3
BIOCELL, Vol.47, No.7, pp. 1595-1609, 2023, DOI:10.32604/biocell.2023.027697
Abstract Background: While the Bushen Yizhi Formula can treat Alzheimer’s disease (AD), the yet to be ascertained
specific mechanism of action was explored in this work. Methods: Different concentrations of the Bushen Yizhi
Formula and amyloid-beta peptide (Aβ) were used to treat rat pheochromocytoma cells (P12) and human
neuroblastoma cells (SH-SY5Y). Cell morphological changes were observed to determine the in vitro cell damage. Cell
Counting Kit (CCK)-8 assay and flow cytometry were employed to identify cell viability and apoptosis/cell cycle,
respectively. Western blotting and immunohistochemistry were employed to measure the expressions of endoplasmic
reticulum stress (ERS)-related proteins (GRP78 and CHOP), p-IRE1α,… More >
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