QILU PAN1,2,#, YAN JIANG1,2,#, LINQIAO LI1,2, XIAOJING DU1, QIAN HAN1, FEIXIANG LING1, ROU LI1, SHUYUAN CHU1,2, LIN MAI1, JIANWEI HUANG1, LIBING MA1,2,*
BIOCELL, Vol.47, No.11, pp. 2495-2502, 2023, DOI:10.32604/biocell.2023.042617
Abstract Introduction: Allergen-specific CD4+ T cells play a central role in autoimmune disorders, allergies and asthma, with Th2-type immunity being the typical functional response of CD4+ T cells. This study aimed to investigate the role of MBD2 in regulating Th2 cell differentiation. Methods: Splenic mononuclear cells were extracted from C57BL/6 mice, and CD4+ T cells were isolated using magnetic beads and confirmed through flow cytometry. Lentivirus was employed to construct MBD2-silenced CD4+ T cells. In vitro experiments were performed to treat splenogenic mononuclear cells and CD4+ T cells with Ovalbumin (OVA), and Th2 cell ratios and IL-4 levels were assessed using… More >
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