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  • Open Access

    ARTICLE

    Hole Cleaning and Critical Transport Rate in Ultra-Deep, Oversized Wellbores

    Yuyao Li1, Mingmin He1, Mingjie Cai1, Shiqian Xu2,*

    FDMP-Fluid Dynamics & Materials Processing, Vol.21, No.4, pp. 799-817, 2025, DOI:10.32604/fdmp.2025.062862 - 06 May 2025

    Abstract In ultra-deep and large well sections, high collapse stresses and diminished annular return velocity present significant challenges to wellbore cleaning. With increasing depth, rising temperature and pressure constrain the regulation of displacement and drilling fluid rheology, impairing the fluid’s capacity to transport cuttings effectively. A precise understanding of cuttings settlement behavior and terminal velocity is therefore essential for optimizing their removal. This study accounts for variations in wellbore temperature and pressure, incorporates non-spherical cuttings and wellbore diameter parameters, and develops accordingly a simplified model to predict terminal settlement velocity. The cuttings carrying ratio is introduced… More > Graphic Abstract

    Hole Cleaning and Critical Transport Rate in Ultra-Deep, Oversized Wellbores

  • Open Access

    ARTICLE

    Characterization of Purged Gas-Liquid Two-Phase Flow in a Molten Salt Regulating Valve

    Shuxun Li1,2, Jianwei Wang1,2,*, Tingjin Ma1, Guolong Deng1,2, Wei Li1,2

    FDMP-Fluid Dynamics & Materials Processing, Vol.21, No.4, pp. 959-988, 2025, DOI:10.32604/fdmp.2025.059570 - 06 May 2025

    Abstract In photothermal power (solar energy) generation systems, purging residual molten salt from pipelines using high-pressure gas poses a significant challenge, particularly in clearing the bottom of regulating valves. Ineffective purging can lead to crystallization of the molten salt, resulting in blockages. To address this issue, understanding the gas-liquid two-phase flow dynamics during high-pressure gas purging is crucial. This study utilizes the Volume of Fluid (VOF) model and adaptive dynamic grids to simulate the gas-liquid two-phase flow during the purging process in a DN50 PN50 conventional molten salt regulating valve. Initially, the reliability of the… More >

  • Open Access

    REVIEW

    Understanding the Molecular Mechanisms of Nitrogen Assimilation in C3 Plants under Abiotic Stress: A Mini Review

    Saif Ullah1,2, Izhar Ali1,2,*

    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1029-1045, 2025, DOI:10.32604/phyton.2025.064608 - 30 April 2025

    Abstract Nitrogen (N) assimilation is crucial for the growth and development of C3 plants, as it converts inorganic N into organic forms, important for protein synthesis, nucleic acids and other vital biomolecules. However, abiotic stressors such as drought, salinity, extreme temperatures and others significantly impact N uptake and utilization, thereby hindering plant growth and development. Recent advances in molecular biology have illuminated the complex networks that govern N assimilation under these stressful conditions, emphasizing the role of transcription factors, regulatory genes, and stress-responsive pathways. This review provides an integrated perspective on the latest research in nitrogen metabolism… More >

  • Open Access

    REVIEW

    Biochar Amendments in Soil: A Sustainable Approach for Mitigating Heavy Metal Stress in Plants

    Shahin Imran1,2,#,*, Mousumi Jahan Sumi3,#, Israt Jahan Harine4, Newton Chandra Paul1, Md. Asif Mahamud5, Rakibul Hasan Md. Rabbi5, Marian Brestic6, Mohammad Saidur Rhaman7,*

    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1073-1109, 2025, DOI:10.32604/phyton.2025.064046 - 30 April 2025

    Abstract Heavy metal (HM) accumulation in soil poses a major hazard to both ecological health and plant growth progressions. Cadmium (Cd), lead (Pb), copper (Cu), chromium (Cr), arsenic (As), zinc (Zn), and nickel (Ni) are examples of HMs that negatively impact the growth and development of plants, resulting in lower agricultural output and food safety concerns. Biochar (BC), a substance rich in carbon that is formed by pyrolyzing natural biomass, has demonstrated remarkable promise in reducing HM stress in polluted soils. Research has shown that BC effectively lowers plant uptake of metals, and enhances soil qualities,… More >

  • Open Access

    ARTICLE

    Metabolomic and Transcriptomic Insights into Enhanced Paclitaxel Biosynthesis in Cultivated Taxus cuspidata

    Dandan Wang*, Jiaxin Chen, Yanwen Zhang

    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1137-1158, 2025, DOI:10.32604/phyton.2025.063894 - 30 April 2025

    Abstract Taxus cuspidata, a rare species of the Taxus genus, and its wild resources are under severe threat. The development of cultivated species has become an important strategy to replace wild species. The objective of this work was to elucidate the differences in secondary metabolite accumulation, particularly in the paclitaxel biosynthesis pathway, between wild and cultivated species. This study employed liquid chromatography-tandem mass spectrometry (LC-MS/MS) and RNA sequencing (RNA-Seq) technologies to conduct integrated metabolomic and transcriptomic analyses of wild and cultivated species of T. cuspidata. The results showed that the content of paclitaxel in cultivated species was significantly higher… More >

  • Open Access

    REVIEW

    Function of Biochar: Alleviation of Heat Stress in Plants and Improvement of Soil Microbial Communities

    Arpita Rani Roy1, Israt Jahan2, Sharah Jabeen Mou2, Md. Farhan Hasin3, Prodipto Bishnu Angon1,*, Rebeka Sultana2, Badhon Mazumder4, Md. Arif Sakil5,*

    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1177-1210, 2025, DOI:10.32604/phyton.2025.063675 - 30 April 2025

    Abstract Global warming is making plants more susceptible to heat stress. Hence, adjustments to crop production systems are required for global food security. Heat stress (HS) poses a threat to the quality of ecosystems and global food security due to its adverse effects on plant development. The degree to which HS affects physiological disruptions, physical harm, and biochemical changes at various growth stages directly correlates with its effects on physiological functions, plant growth, and crop production. One promising approach is soil modification using biochar, which enhances soil health and promotes the development of microbial communities, ultimately… More >

  • Open Access

    ARTICLE

    Molecular Cloning, Subcellular Localization and Expression Analyses of PdbHLH57 Transcription Factor in Colored-Leaf Poplar

    Yuhang Li1, Li Sun1, Tao Wang1, Bingjun Yu2, Zhihong Gao3, Xiaochun Shu1, Tengyue Yan1, Weibing Zhuang1,2,*, Zhong Wang1,*

    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1211-1223, 2025, DOI:10.32604/phyton.2025.063647 - 30 April 2025

    Abstract bHLH transcription factors, widely exist in various plants, and are vital for the growth and development of these plants. Among them, many have been implicated in anthocyanin biosynthesis across various plants. In the present study, a PdbHLH57 gene, belonging to the bHLH IIIf group, was characterized, which was isolated and cloned from the colored-leaf poplar ‘Zhongshancaiyun’ (ZSCY). The cDNA sequence of PdbHLH57 was 1887 base pairs, and the protein encoded by PdbHLH57 had 628 amino acids, the isoelectric point and molecular weight of which were 6.26 and 69.75 kDa, respectively. Through bioinformatics analysis, PdbHLH57 has been classified… More >

  • Open Access

    ARTICLE

    Aphicidal and Antimicrobial Activities of Salvia rosmarinus Essential Oil and Its Major Compound, 1,8-Cineole

    Ghizlane Houzi1, Aimad Allali2,3,*, Amine Elbouzidi4,*, Mohamed Taibi4, Mohamed Chebaibi3,5, Ben Khada Zineb6, Ramzi A. Mothana7, Mohammed F. Hawwal7, Rachid Flouchi3,8, Abdeslam Asehraou9, Amal Lahkimi2, Soad Khal-Layoun1

    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1239-1251, 2025, DOI:10.32604/phyton.2025.063021 - 30 April 2025

    Abstract This work uses GC-MS to analyze the bioactive compounds of Salvia rosmarinus essential oils (SREO) and evaluates their antibacterial, antifungal, and insecticidal effects, as well as the major component, 1,8-cineole. Chemical analysis identified 16 compounds accounting for 99.19% of the oil’s total content, with 1,8-cineole (33.17%), camphor (16.53%), α-pinene (14.46%), and camphene (8.14%) as the major constituents. Antimicrobial activities were assessed against pathogenic strains using minimal inhibit concentration (MIC) and minimum bactericidal concentration (MBC) assays. SREO exhibited a minimum MIC of 0.128% against P. aeruginosa, while 1,8-cineole showed a minimum MIC of 2.06% against the same strain,… More >

  • Open Access

    ARTICLE

    Reprogramming the Metabolome of Centella asiatica (L.) Urban Callus: Profiling of Newly Synthesized Cryptic Anthocyanins Triggered by LED Light Exposure

    Claude Y. Hamany Djande, Paul A. Steenkamp, Ian A. Dubery*

    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1269-1286, 2025, DOI:10.32604/phyton.2025.062392 - 30 April 2025

    Abstract Centella asiatica L., a medicinal herb, has attracted substantial interest in research as well as commercial domains due to its bioactive compounds which include the pentacyclic triterpenoid centellosides, and in addition, hydroxy. In addition, hydroxycinnamic acid conjugates as well as flavonoids. The latter is the major class of secondary plant metabolites and comprises various subclasses, including anthocyanidins. Anthocyanins are rarely reported in extracts from C. asiatica and differ structurally due to a flavylium (2-phenylchromenylium) ion that carries a positive charge at the oxygen atom of the C-ring of the basic flavonoid structure. Callus of C. asiatica was initiated… More >

  • Open Access

    ARTICLE

    Drought-Ready Soybean Resilience: Molecular Marker-Based Screening and Selection of Superior Genotypes

    Muhammad Imran Khan1, Fahad Masoud Wattoo1, Kamran Shehzad Bajwa2,*, Zahid Akram1, Sabahat Noor2, Zaheer Abbas2, Rashid Mehmood Rana1, Muhammad Tahir Akram3, Shaukat Ali2, Rai Muhammad Amir4, Ghulam Muhammad Ali5

    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1299-1318, 2025, DOI:10.32604/phyton.2025.062323 - 30 April 2025

    Abstract The agricultural sector is notably affected by climate change, especially soybeans, which may face diminished yields because of severe water shortages. The evaluation of germplasm at morphological and molecular levels is an important pre-breeding step for crop improvement. This study employed 10 simple sequence repeat (SSR) markers to examine 60 soybean genotypes in the quest for drought-resistant lines during 2022–23. The results of the screening experiment (PEG-6000) revealed that the soybean genotypes SPS13, SPS195, PGRB83, and 39982 exhibited significant correlations in growth parameters. The results of molecular characterization indicated that five out of ten molecular… More >

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