NIMA HEMMAT¹, MOHAMMAD AMIN DOUSTVANDI¹, ZAHRA ASADZADEH¹, AHAD MOKHTARZADEH¹, BEHZAD BARADARAN^1,4,*, HOSSEIN BANNAZADEH BAGHI^1,2,3,*

Oncology Research, Vol.29, No.2, pp. 141-148, 2021, DOI:10.32604/or.2022.023346

Abstract Cervical cancer is a growing global disease in developing countries. Persistent infection with human papillomaviruses (HPV) is an essential causative agent in this type of cancer. Several studies demonstrate HPV E5 oncoprotein can impress the normal life cycle of HPV-infected cells by targeting some pivotal cellular signaling pathways, such as the epidermal growth factor receptor (EGFR) signaling pathway. In this study, we used E5-siRNA to knockdown that essential oncogene and considered the effect of E5 silencing on proliferation, apoptosis, cell cycle, apoptosis-related gene expression, and the initiator of the EGFR signaling pathway in cervical cancer cells. The results demonstrate that… More >

Kalaivani Muniandy¹, Zuhaida Asra Ahmad^1,4, Sylvia Annabel Dass¹, Shaharum Shamsuddin², Nethia Mohana Kumaran³, Venugopal Balakrishnan^1,*

Oncologie, Vol.23, No.2, pp. 279-291, 2021, DOI:10.32604/Oncologie.2021.015969

Abstract Spheroids are generally self-assembled cells with the ability to generate their extracellular matrix, including the complex cell-matrix and the cell-cell interactions that resemble the functional characteristics of the corresponding tissue in vivo. The study aimed to develop a three-dimensional (3D) spheroid system for the cervical cancer cell lines, HeLa (HPV18), CaSki (HPV16), SiHa (HPV16), C33A (non-HPV), HT3 (non-HPV) as well as to identify its biological activity in the extracellular form. For the formation of the cervical cancer spheroids, the liquid overlay approach was applied, followed by embedding to the bovine collagen I matrix. Spheroid formation using the liquid overlay approach… More >