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  • Open Access


    3D Bio-Plotted Composite Scaffold Made of Collagen Treated Hydroxyapatite-Tricalciumphosphate for Rabbit Tibia Bone Regeneration

    Pranav S. Sapkal1*, Abhaykumar M. Kuthe1, Divya Ganapathy2, Shantanu C. Mathankar3, Sudhanshu Kuthe4

    Molecular & Cellular Biomechanics, Vol.13, No.2, pp. 115-136, 2016, DOI:10.3970/mcb.2016.013.131

    Abstract Biphasic calcium phosphate scaffolds with 20/80 HA/TCP ratio were fabricated using the 3D-Bioplotting system to heal critical size defects in rabbit tibia bone. Four different architectures were printed in a layer by layer fashion with lay down patterns viz. (a) 0°– 90°, (b) 0°– 45°– 90°– 135°, (c) 0°–108°– 216° and (d) 0°– 60°– 120°. After high-temperature sintering scaffolds were coated with collagen and were further characterized by (FTIR) Fourier Transform Infrared Spectroscopy, (SEM) Scanning Electron Microscopy, (XRD) X-Ray diffraction, Porosity analysis and Mechanical testing. Scaffold samples were tested for its ability to induce cytotoxicity in Balb/c 3T3 cells at… More >

  • Open Access


    Intracellular Calcium Waves in Bone Cell Networks under Single Cell Nanoindentation

    X. Edward Guo∗,†,‡, Erica Takai∗,‡, Xingyu Jiang§, Qiaobing Xu§, George M. Whitesides§, James T. Yardley, Clark T. Hung*, Eugene M. Chow||, Thomas Hantschel∗∗, Kevin D. Costa

    Molecular & Cellular Biomechanics, Vol.3, No.3, pp. 95-108, 2006, DOI:10.3970/mcb.2006.003.095

    Abstract In this study, bone cells were successfully cultured into a micropatterned network with dimensions close to that of in vivo osteocyte networks using microcontact printing and self-assembled monolyers (SAMs). The optimal geometric parameters for the formation of these networks were determined in terms of circle diameters and line widths. Bone cells patterned in these networks were also able to form gap junctions with each other, shown by immunofluorescent staining for the gap junction protein connexin 43, as well as the transfer of gap-junction permeable calcein-AM dye. We have demonstrated for the first time, that the intracellular calcium response of a… More >

  • Open Access


    Birefringence Simulations of Calcium Fluoride Single Crystal Used as Chamber Window of Gas Laser Light Source

    Yuta Kitamura1, Noriyuki Miyazaki1, Takahito Kumazaki2, Naoto Nagakura3, Yasuhiro Hashimoto3, Isao Masada3

    CMES-Computer Modeling in Engineering & Sciences, Vol.68, No.2, pp. 151-166, 2010, DOI:10.3970/cmes.2010.068.151

    Abstract CaF2 single crystal is used as high performance optical elements. We developed an analysis system for simulating birefringence of CaF2 single crystal used as a chamber window of a gas laser light source. The analysis system consists of a stress analysis and a birefringence analysis. In the stress analysis, the finite element method was applied to obtain the mechanical stress caused by a window holder and gas pressure. In the birefringence analysis, the photo-elastic effect gives the change of refractive indices, from which the optical path difference and the fast axis are calculated by using the average stress method. The… More >

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