Pranav S. Sapkal^1*, Abhaykumar M. Kuthe¹, Divya Ganapathy², Shantanu C. Mathankar³, Sudhanshu Kuthe⁴

Molecular & Cellular Biomechanics, Vol.13, No.2, pp. 115-136, 2016, DOI:10.3970/mcb.2016.013.131

Abstract Biphasic calcium phosphate scaffolds with 20/80 HA/TCP ratio were fabricated using the 3D-Bioplotting system to heal critical size defects in rabbit tibia bone. Four different architectures were printed in a layer by layer fashion with lay down patterns viz. (a) 0°– 90°, (b) 0°– 45°– 90°– 135°, (c) 0°–108°– 216° and (d) 0°– 60°– 120°. After high-temperature sintering scaffolds were coated with collagen and were further characterized by (FTIR) Fourier Transform Infrared Spectroscopy, (SEM) Scanning Electron Microscopy, (XRD) X-Ray diffraction, Porosity analysis and Mechanical testing. Scaffold samples were tested for its ability to induce cytotoxicity… More >

MAURO RODRIGO SURENCISKI^*, EDUARDO ALBERTO FLACHSLAND, GRACIELA TERADA, LUIS AMADO MROGINSKI, HEBE YOLANDA REY

BIOCELL, Vol.36, No.1, pp. 31-36, 2012, DOI:10.32604/biocell.2012.36.031

Abstract The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and More >

García Osuna HT, A Benavides Mendoza, L Escobedo Bocardo, JA Villarreal Quintanilla, E Cornejo Oviedo

Phyton-International Journal of Experimental Botany, Vol.80, pp. 175-179, 2011, DOI:10.32604/phyton.2011.80.175

Abstract Turbinicarpus valdezianus is a species under special protection, according to the current law NOM-059-ECOL-2010. It spreads preferably through shoot proliferation in vitro. A common problem associated with the propagation of this species by tissue culture is the hyperhydricity or excess of water accumulation in the tissues of shoots explants. The literature on this topic indicates that such response is related with oxidative stress. Because of this, the effects of inhibitors of the gibberellins [paclobutrazol (PBZ) and calcium prohexadione (PCa)] and salicylic (SA) and benzoic acids (BA) were tested to diminish the hiperhydrycity of the sprouts, which was… More >

Bo Huo, Man Hu, Ping Li

The International Conference on Computational & Experimental Engineering and Sciences, Vol.18, No.4, pp. 103-104, 2011, DOI:10.3970/icces.2011.018.103

Abstract It has been widely accepted that movement of human body causes the fluid flow through pores or channels inside bone and subsequently on osteoblasts on the surface of trabeculae and osteocytes inside lacunae. The mechanism of calcium response in a bone cell and calcium transfer between bone cells is critical in understanding the communication between bone cells and calcium deposition on bone matrix. Our previous works have demonstrated that when micropatterned osteoblastic cell network with gap junctions was exposed to fluid flow, extracellular ATP diffusion following the activation of calcium response in neighboring cells plays… More >

Yuta Kitamura¹, Noriyuki Miyazaki¹, Takahito Kumazaki², Naoto Nagakura³, Yasuhiro Hashimoto³, Isao Masada³

CMES-Computer Modeling in Engineering & Sciences, Vol.68, No.2, pp. 151-166, 2010, DOI:10.3970/cmes.2010.068.151

Abstract CaF₂ single crystal is used as high performance optical elements. We developed an analysis system for simulating birefringence of CaF₂ single crystal used as a chamber window of a gas laser light source. The analysis system consists of a stress analysis and a birefringence analysis. In the stress analysis, the finite element method was applied to obtain the mechanical stress caused by a window holder and gas pressure. In the birefringence analysis, the photo-elastic effect gives the change of refractive indices, from which the optical path difference and the fast axis are calculated by using the More >

S. Sun¹, S. Lipsky¹, M. Cho¹

Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 213-213, 2006, DOI:10.32604/mcb.2006.003.213

Abstract This article has no abstract. More >

X. Edward Guo^∗,†,‡, Erica Takai^∗,‡, Xingyu Jiang^§, Qiaobing Xu^§, George M. Whitesides^§, James T. Yardley^¶, Clark T. Hung^*, Eugene M. Chow^||, Thomas Hantschel^∗∗, Kevin D. Costa^∗

Molecular & Cellular Biomechanics, Vol.3, No.3, pp. 95-108, 2006, DOI:10.3970/mcb.2006.003.095

Abstract In this study, bone cells were successfully cultured into a micropatterned network with dimensions close to that of in vivo osteocyte networks using microcontact printing and self-assembled monolyers (SAMs). The optimal geometric parameters for the formation of these networks were determined in terms of circle diameters and line widths. Bone cells patterned in these networks were also able to form gap junctions with each other, shown by immunofluorescent staining for the gap junction protein connexin 43, as well as the transfer of gap-junction permeable calcein-AM dye. We have demonstrated for the first time, that the intracellular More >