Barresi O^1,2, VM Chiocchio^1,2, RS Lavado²

Phyton-International Journal of Experimental Botany, Vol.87, pp. 14-17, 2018, DOI:10.32604/phyton.2018.87.014

Abstract Previous studies in soils of the Pampas region indicate the prevalence of calcium phosphates within the very complex mix of phosphorus (P) compounds in the soils. We studied the changes in P fractions in a punctual situation in the Pampas region. The sampling was carried out in a farm located near the city of Junín (-34.585; -60.9589) and the soil was Junín series Typic Hapludoll. The farm was devoted to grazing and sporadic crops, but from the last 30 years changed to continuous agriculture. At no time fertilization matched nutrients removal by crops. The soil More >

Benjamin J. King¹, Caleb J. Seufert¹, Zhamshid Okhunov², Nazih Khater³, D. Duane Baldwin³, Peter W. Callas¹, Kevan M. Sternberg¹

Canadian Journal of Urology, Vol.24, No.1, pp. 8641-8645, 2017

Abstract Introduction: To identify factors associated with stone composition in patients undergoing percutaneous nephrolithotomy (PCNL).
Material and methods: A retrospective analysis of patients who underwent PCNL at two academic institutions between 2002 and 2014. Stone composition, stone characteristics based on non-contrast computer tomography (NCCT), patient demographics, and the S.T.O.N.E nephrolithometry scores were compared. Stones were characterized as either infection or metabolic. Metabolic stones were classified as calcium phosphate-containing and all others.
Results: A total of 192 renal units underwent PCNL. Retrieved stones were found to be 75% (144) metabolic and 25% (48) infection by stone analysis. Of the metabolic… More >

Ping Wu, Huatao Quan, Jing Kang, Jian He, Shi Luo, Chubo Xie, Jing Xu, Yaoyun Tang, Suping Zhao

Oncology Research, Vol.25, No.9, pp. 1479-1488, 2017, DOI:10.3727/096504017X14886420642823

Abstract Hypopharyngeal cancer (HPC) frequently presents at an advanced stage and displays early submucosal spread, resulting in a poor prognosis. It is among the worst of all cancers in the head and neck subsites. Therefore, detection of HPC at an earlier stage would be beneficial to patients. In this study, we used differential in-gel electrophoresis (DIGE) and two-dimensional polyacrylamide gel electrophoresis (2-DE) proteomics analysis to identify the potential biomarkers for HPC. Among the differential proteins identified, calcium-binding protein S100A9 was overexpressed in HPC tissues compared with normal adjacent tissues, and S100A9 expression in metastatic tissues and… More >

Antonello Di Paolo¹, Paola Orlandi¹, Teresa Di Desidero, Romano Danesi, Guido Bocci

Oncology Research, Vol.25, No.7, pp. 1129-1140, 2017, DOI:10.3727/096504017X14841698396900

Abstract The combination of folinate salts to 5-fluoruracil (5-FU)-based schedules is an established clinical routine in the landscape of colorectal cancer treatment. The aim of this study was to investigate the pharmacological differences between the sequential administration of folinate salts (1 h before, as in clinical routine) followed by 5-FU and the simultaneous administration of both drugs. Proliferation and apoptotic assays were performed on human colon cancer cells exposed to 5-FU, calcium (CaLV), or disodium (NaLV) levofolinate or their simultaneous and sequential combination for 24 and 72 h. TYMS and SLC19A1 gene expression was performed with… More >

Ramírez H¹, JC Sánchez-Canseco¹, VM Zamora-Villa², JH Rancaño-Arrioja³

Phyton-International Journal of Experimental Botany, Vol.86, pp. 282-289, 2017, DOI:10.32604/phyton.2017.86.282

Abstract It is well established that the climatic change is altering yield in fruit trees. This adversity reflects, among others, extreme temperature modifications in northern Mexico. This effect modifies negatively the physiology of apple trees (Malus domestica Borkh.), originating excessive shoot growth and a decrease in flower bud formation. In this study, Prohexadione-Ca (P-Ca), 6-benzyl amino purine (BAP) and N6-furfuryladenine (kinetin) were evaluated on shoot and reproductive growth in Golden Delicious apple trees in Arteaga Coah., Mexico. A completely randomized design with 12 treatments was used. These were: Control, Prohexadione calcium (Ca-P) at doses of 150, 300… More >

Muñoz FF, VE Ruiz, CA Bouzo

Phyton-International Journal of Experimental Botany, Vol.86, pp. 131-136, 2017, DOI:10.32604/phyton.2017.86.131

Abstract The aim of this work was to study the effect of preharvest foliar spraying with calcium nitrate solutions on the fruit melon quality. Treatments included a combination of five solutions with Ca(NO₃)₂ (0.0; 1.3; 2.6; 5.2 and 10.5 g/L) and two postharvest storage environments [(24.0 ± 1.5 °C and 6.4 ± 0,8 mbar of vapor pressure deficit (VPD), and 10.0 ± 0.5 °C and 3.0 ± 0.5 mbar (VPD)]. Fruits stored at 24 °C for 11 days had the lowest water loss with treatments 1.3 and 2.6 g/L Ca (NO₃)₂. It was observed that at both More >

Guerrero-Prieto VM¹, DI Berlanga-Reyes², JL Jacobo-Cuellar¹, C Guigón-Lopez³, DL Ojeda-Barrios⁴, GD Ávila-Quezada⁴, A Núñez-Barrios⁴, OA Hernández-Rodríguez⁴

Phyton-International Journal of Experimental Botany, Vol.86, pp. 74-78, 2017, DOI:10.32604/phyton.2017.86.074

Abstract Calcium content and damage severity of Penicillium expansum because of its high concentration on “Red Delicious” postharvest apples were evaluated during the 2012-2013 winter in Cuauhtemoc, Chih. Mexico. Fruit weight, diameter, total soluble solids, pulp firmness and starch index were also determined. Penicillium expansum was inoculated into two wounds of eight mm diameter and 10 mm depth, on a total of 20 apple fruits per treatment. The inoculum was 0.2 mL of a suspension containing 1×10⁸ conidia/mL. After inoculation, apple fruit was stored at 0 °C and 90% relative humidity during five weeks. Treatments were: apple fruit with More >

Agnieszka Sobczak-Kupiec¹, Klaudia Pluta¹, Dagmara Malina^1*, Bożena Tyliszczak²

Journal of Renewable Materials, Vol.5, No.3-4, pp. 180-188, 2017, DOI:10.7569/JRM.2017.634106

Abstract In recent years, the development of composite biomaterials has been the subject of very intensive research. The elaboration of technology for manufacturing new biomaterials will allow their practical implementation and adaptation to changing market needs. One of the key components in the developed composite materials will be natural origin hydroxyapatite (HAp) and tricalcium phosphate (TCP) obtained from bone products. In this study, preparation and detailed characterization of bone-derived calcium phosphates as a component of biomaterial composites is proposed. This novel method of obtaining hydroxyapatite for biomedical applications allows the obtainment of a material with expected More >

Pranav S. Sapkal^1,*, Abhaykumar M. Kuthe¹, Shantanu Mathankar², Akash A. Deshmukh

Molecular & Cellular Biomechanics, Vol.14, No.2, pp. 125-136, 2017, DOI:10.3970/mcb.2017.014.123

Abstract β-TCP-Zirconia scaffolds with different architectures were fabricated by means of 3D-Bioplotting in order to enhance the mechanical and in-vitro ability of the scaffold to heal large size bone defects. In the present study scaffold architecture with different strand orientations (0°-90°, 0°-45°-135°-180°, 0°-108°-216° and 0°-72°-144°-36°-108°) were fabricated, characterized and evaluated for mechanical strength and cell proliferation ability. β-TCP powder (25 µm) and PVA (Polyvinyl Alcohol) was acquired from Fisher Scientific, India. Zirconia (18 to 32 µm) was procured from Lobachemie, India. In brief 7.5%, PVA in distilled water was used as a binder and was mixed… More >

Juanita BUSTAMANTE^1,*, Analía CZERNICZYNIEC², Silvia LORES-ARNAIZ²

BIOCELL, Vol.40, No.1, pp. 11-14, 2016, DOI:10.32604/biocell.2016.40.011

Abstract The suppressive effects of ketamine on intracellular calcium has been reported in a variety of cells although the mechanisms involved are not well understood. The aim of this work was to evaluate the ketamine effect on the mitochondrial Ca²⁺ accumulation and the cellular Ca²⁺ mobilization using FLUO4-AM and flow cytometry. The results showed that mitochondria from ketamine injected animals presented a lower ability to retain calcium at concentrations higher than 20 μM, as compared with controls (saline injected animals). In addition, ketamine showed a significant decreased KCl-induced intracellular calcium concentration. KCl increased calcium influx through More >