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  • Open Access


    Effect of preharvest foliar sprays of calcium nitrate on melon fruit quality

    Muñoz FF, VE Ruiz, CA Bouzo

    Phyton-International Journal of Experimental Botany, Vol.86, pp. 131-136, 2017, DOI:10.32604/phyton.2017.86.131

    Abstract The aim of this work was to study the effect of preharvest foliar spraying with calcium nitrate solutions on the fruit melon quality. Treatments included a combination of five solutions with Ca(NO3)2 (0.0; 1.3; 2.6; 5.2 and 10.5 g/L) and two postharvest storage environments [(24.0 ± 1.5 °C and 6.4 ± 0,8 mbar of vapor pressure deficit (VPD), and 10.0 ± 0.5 °C and 3.0 ± 0.5 mbar (VPD)]. Fruits stored at 24 °C for 11 days had the lowest water loss with treatments 1.3 and 2.6 g/L Ca (NO3)2. It was observed that at both 24 °C and 10… More >

  • Open Access


    Calcium content on apple fruit influences the severity of Penicillium expansum

    Guerrero-Prieto VM1, DI Berlanga-Reyes2, JL Jacobo-Cuellar1, C Guigón-Lopez3, DL Ojeda-Barrios4, GD Ávila-Quezada4, A Núñez-Barrios4, OA Hernández-Rodríguez4

    Phyton-International Journal of Experimental Botany, Vol.86, pp. 74-78, 2017, DOI:10.32604/phyton.2017.86.074

    Abstract Calcium content and damage severity of Penicillium expansum because of its high concentration on “Red Delicious” postharvest apples were evaluated during the 2012-2013 winter in Cuauhtemoc, Chih. Mexico. Fruit weight, diameter, total soluble solids, pulp firmness and starch index were also determined. Penicillium expansum was inoculated into two wounds of eight mm diameter and 10 mm depth, on a total of 20 apple fruits per treatment. The inoculum was 0.2 mL of a suspension containing 1×108 conidia/mL. After inoculation, apple fruit was stored at 0 °C and 90% relative humidity during five weeks. Treatments were: apple fruit with high (2.28… More >

  • Open Access


    Ketamine effect on intracellular and mitochondrial calcium mobilization


    BIOCELL, Vol.40, No.1, pp. 11-14, 2016, DOI:10.32604/biocell.2016.40.011

    Abstract The suppressive effects of ketamine on intracellular calcium has been reported in a variety of cells although the mechanisms involved are not well understood. The aim of this work was to evaluate the ketamine effect on the mitochondrial Ca2+ accumulation and the cellular Ca2+ mobilization using FLUO4-AM and flow cytometry. The results showed that mitochondria from ketamine injected animals presented a lower ability to retain calcium at concentrations higher than 20 μM, as compared with controls (saline injected animals). In addition, ketamine showed a significant decreased KCl-induced intracellular calcium concentration. KCl increased calcium influx through cellular depolarization. According to the… More >

  • Open Access


    Changes in soil phosphorus fractions caused by cropping without nutrient reposition. A case study

    Barresi O1,2, VM Chiocchio1,2, RS Lavado2

    Phyton-International Journal of Experimental Botany, Vol.87, pp. 14-17, 2018, DOI:10.32604/phyton.2018.87.014

    Abstract Previous studies in soils of the Pampas region indicate the prevalence of calcium phosphates within the very complex mix of phosphorus (P) compounds in the soils. We studied the changes in P fractions in a punctual situation in the Pampas region. The sampling was carried out in a farm located near the city of Junín (-34.585; -60.9589) and the soil was Junín series Typic Hapludoll. The farm was devoted to grazing and sporadic crops, but from the last 30 years changed to continuous agriculture. At no time fertilization matched nutrients removal by crops. The soil P fractions were determined using… More >

  • Open Access


    Physical Regulation of Human Mesenchymal Stem Cells through Altered Calcium Dynamics

    S. Sun1, S. Lipsky1, M. Cho1

    Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 213-213, 2006, DOI:10.32604/mcb.2006.003.213

    Abstract This article has no abstract. More >

  • Open Access


    Calcium Response and Transfer in Bone Cell Network with or without Gap Junctions under Mechanical Stimulation

    Bo Huo, Man Hu, Ping Li

    The International Conference on Computational & Experimental Engineering and Sciences, Vol.18, No.4, pp. 103-104, 2011, DOI:10.3970/icces.2011.018.103

    Abstract It has been widely accepted that movement of human body causes the fluid flow through pores or channels inside bone and subsequently on osteoblasts on the surface of trabeculae and osteocytes inside lacunae. The mechanism of calcium response in a bone cell and calcium transfer between bone cells is critical in understanding the communication between bone cells and calcium deposition on bone matrix. Our previous works have demonstrated that when micropatterned osteoblastic cell network with gap junctions was exposed to fluid flow, extracellular ATP diffusion following the activation of calcium response in neighboring cells plays more important roles in calcium… More >

  • Open Access


    Valorization of a Good Bioceramic from Moroccan Waste Fish Bone by a Heat Treatment Method

    Kaoutar Kara1,*, Fatiha Ouanji2, El Mostapha LOTFI1, Mohammed El Mahi1, Mohamed Kacimi2

    Journal of Renewable Materials, Vol.7, No.1, pp. 1-7, 2019, DOI:10.32604/jrm.2019.00016

    Abstract A tricalcium phosphate (TCP) material was produced from sardine and mackerel waste using a heat treatment method after a Soxhlet extraction to obtain the non-soluble portion of fish waste. The bones were annealed at temperatures between 400°C and 1200°C. The thermal analysis (TG-DTA) was carried out to investigate the thermal stability of TCP and to confirm the removal of organic matter from the raw fish. The calcined bones were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and field emission scanning electron microscopy (FE-SEM). The calcium to phosphorous weight ratio was determined by ICP- AES. FT-IR and XRD… More >

  • Open Access


    Studies on Bone-Derived Calcium Phosphate Materials

    Agnieszka Sobczak-Kupiec1, Klaudia Pluta1, Dagmara Malina1*, Bożena Tyliszczak2

    Journal of Renewable Materials, Vol.5, No.3-4, pp. 180-188, 2017, DOI:10.7569/JRM.2017.634106

    Abstract In recent years, the development of composite biomaterials has been the subject of very intensive research. The elaboration of technology for manufacturing new biomaterials will allow their practical implementation and adaptation to changing market needs. One of the key components in the developed composite materials will be natural origin hydroxyapatite (HAp) and tricalcium phosphate (TCP) obtained from bone products. In this study, preparation and detailed characterization of bone-derived calcium phosphates as a component of biomaterial composites is proposed. This novel method of obtaining hydroxyapatite for biomedical applications allows the obtainment of a material with expected parameters. In this study, pork… More >

  • Open Access


    3D Bio-Plotted Tricalcium Phosphate/Zirconia Composite Scaffolds to Heal Large Size Bone Defects

    Pranav S. Sapkal1,*, Abhaykumar M. Kuthe1, Shantanu Mathankar2, Akash A. Deshmukh

    Molecular & Cellular Biomechanics, Vol.14, No.2, pp. 125-136, 2017, DOI:10.3970/mcb.2017.014.123

    Abstract β-TCP-Zirconia scaffolds with different architectures were fabricated by means of 3D-Bioplotting in order to enhance the mechanical and in-vitro ability of the scaffold to heal large size bone defects. In the present study scaffold architecture with different strand orientations (0°-90°, 0°-45°-135°-180°, 0°-108°-216° and 0°-72°-144°-36°-108°) were fabricated, characterized and evaluated for mechanical strength and cell proliferation ability. β-TCP powder (25 µm) and PVA (Polyvinyl Alcohol) was acquired from Fisher Scientific, India. Zirconia (18 to 32 µm) was procured from Lobachemie, India. In brief 7.5%, PVA in distilled water was used as a binder and was mixed with 10 grams of (70/30)… More >

  • Open Access


    Differential Responses of Cultured MC3T3-E1 Cells to Dynamic and Static Stimulated Effect of Microgravity in Cell Morphology, Cytoskeleton Structure and Ca2+ Signaling

    Mingzhi Luo1,2, Peili Yu1, Yang Jin3, Zhili Qian1, Yue Wang1, Jingjing Li1, Peng Shang2*, Linhong Deng1*

    Molecular & Cellular Biomechanics, Vol.13, No.2, pp. 137-157, 2016, DOI:10.3970/mcb.2016.013.155

    Abstract Random positioning machine (RPM) and diamagnetic levitation are two essential ground-based methods used to stimulate the effect of microgravity in space life science research. However, the force fields generated by these two methods are fundamentally different, as RPM generates a dynamic force field acting on the surface in contact with supporting substrate, whereas diamagnetic levitation generates a static force field acting on the whole body volume of the object (e.g. cell). Surprisingly, it is hardly studied whether these two fundamentally different force fields would cause different responses in mammalian cells. Thus we exposed cultured MC3T3-E1 osteoblasts to either dynamically stimulated… More >

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