Luyao Jiang^1,#, Longsheng Fu^2,#, Shaofeng Xiong^2,3, Guosheng Cao⁴, Yanqin Mei^2,3, Yaoqi Wu², Jin Chen^1,*, Yanni LV^2,5,6,*

BIOCELL, Vol.49, No.9, pp. 1749-1769, 2025, DOI:10.32604/biocell.2025.068507 - 25 September 2025

Abstract Background: After ischemic stroke, neutrophils hyperactivate, increasing in number and worsening inflammation, causing neural damage. Prior scRNA-seq showed Lrg1 modulates cells subsentence to cerebral ischemia-reperfusion injury, but its mechanism in regulating neutrophil accumulation/differentiation post-injury is unclear. Methods: Lrg1 knockout impact on neutrophil accumulation was assessed via immunofluorescence and western blot. Three-dimensional reconstruction of immunofluorescent staining analyzed cell-cell interactions among neutrophils and microglia. scRNA-seq of WT and Lrg1^-/- mice from GSE245386 and GSE279462 was conducted. Each group conducted oxidative phosphorylation scoring via Gene Set Enrichment Analysis (GSEA), while Metascape was employed to perform GO and KEGG enrichment… More > Graphic Abstract

Siliang Liu^1,#, Hong Tang^2,#, Ying Xia¹, Zhengtao Yu¹, Ning Gao^1,*

BIOCELL, Vol.49, No.7, pp. 1245-1264, 2025, DOI:10.32604/biocell.2025.064396 - 25 July 2025

Abstract Objectives: NOD-like receptor family pyrin domain-containing (NLRP) 1-mediated pyroptosis plays a key role in the pathogenesis of cerebral ischemia-reperfusion injury (CIRI). C-Myc is reported to play a major role in CIRI. However, the mechanism remains unclear. This study aimed to investigate whether c-Myc affects CIRI by regulating Serine/Arginine-rich Splicing Factor 1 (SRSF1)/NLRP1-mediated pyroptosis. Methods: Oxygen-glucose deprivation/reperfusion (OGD/R) induced neuroblastoma cells for the establishment of an in vitro CIRI model. The levels of c-Myc and SRSF1, cell viability, the expression of pyroptosis-related factors, and the interaction between SRSF1 and NLRP1 were evaluated. Results: The expression of c-Myc and… More >

LONGSHENG FU¹, JINFANG HU¹, FENG SHAO², YAOQI WU¹, WEI BAI³, MINGJIN JIANG³, HAO CHEN⁴, LIHUA CHEN², YANNI LV^1,*

BIOCELL, Vol.47, No.8, pp. 1869-1883, 2023, DOI:10.32604/biocell.2023.028602 - 28 August 2023

Abstract Background: Biochanin A is an excellent dietary isoflavone that has the concomitant function of both medicine and foodstuff. The attenuation function of biochanin A on blood-brain barrier (BBB) damage induced by cerebral ischemia-reperfusion remains unclear. Methods: C57BL/6 mice were subjected to 1 h middle cerebral artery occlusion (MCAO) followed by 24 h reperfusion. The infarct volume of the brain was stained by TTC, while leakage of the brain was quantitatively stained by Evans blue, and the neurologic deficit score was measured. Microglial-induced morphologic changes were observed via immunofluorescence staining, and rolling and adhering leukocytes in… More >

JINFANG HU^1,#, JIANGEN AO^2,#, LONGSHENG FU^1,#, YAOQI WU¹, FENG SHAO³, TIANTIAN XU¹, MINGJIN JIANG⁴, SHAOFENG XIONG¹, YANNI LV^1,*

BIOCELL, Vol.47, No.7, pp. 1611-1622, 2023, DOI:10.32604/biocell.2023.028684 - 21 June 2023

Abstract Background: The attenuation function of Dalbergia odorifera leaves on cerebral ischemia-reperfusion (I/R) is little known. The candidate targets for the Chinese herb were extracted from brain tissues through the high-affinity chromatography. The molecular mechanism of D. odorifera leaves on cerebral I/R was investigated. Methods: Serial affinity chromatography based on D. odorifera leaves extract (DLE) affinity matrices were applied to find specific binding proteins in the brain tissues implemented on C57BL/6 mice by intraluminal middle cerebral artery occlusion for 1 h and reperfusion for 24 h. Specific binding proteins were subjected to mass-spectrometry to search for the differentially expressed… More >

JING JIN^1,#, XINGHUA WANG^2,#, XIAOXIAO ZHENG³, JIAHUA LAN³, LI ZHENG³, YING CAI³, HUI CHEN⁴, HONGWEI WANG^5,*, LIFANG ZHENG^6,*

BIOCELL, Vol.47, No.3, pp. 619-626, 2023, DOI:10.32604/biocell.2023.026016 - 03 January 2023

Abstract Ischemic cerebrovascular disease is a leading cause of death globally and is often exacerbated by cerebral ischemic/reperfusion injury (CIRI). The exact mechanisms underlying I/R injury are unclear. In this study, we aimed to determine the role of m⁶A-modified methylase complex methyltransferase-like 3 (METTL3) in cerebral ischemia-reperfusion (I/R) injury. We found that m6A and METTL3 levels increased in OGD/RX-induced mouse astrocytes-cerebellar (MA-C) and the brain of middle cerebral artery occlusion (MCAO) model mice. METTL3 siRNA treatment reduced OGD-RX-induced MAC cell viability and proliferation, which increased with METTL3 over-expression. Flow cytometry analysis showed that silencing METTL3 significantly enhanced More >