Yunfeng Chen1,2,3,†,*, Jiexi Liao4,†, Zhou Yuan1, Kaitao Li4, Baoyu Liu4, Lining Arnold Ju4,5,6, Cheng Zhu1,2,4,*
Molecular & Cellular Biomechanics, Vol.16, No.3, pp. 211-223, 2019, DOI:10.32604/mcb.2019.07267
Abstract Force plays critical roles in cell adhesion and mechano-signaling, partially by regulating the dissociation rate, i.e., off-rate, of receptor-ligand bonds. However, the mechanism of such regulation still remains elusive. As a controversial topic of the field, when measuring the “off-rate vs. force” relation of the same molecular system, different dynamic force spectroscopy (DFS) assays (namely, force-clamp and force-ramp assays) often yield contradictive results. Such discrepancies hurdled our further understanding of molecular binding, and casted doubt on the existing theoretical models. In this work, we used a live-cell DFS technique, biomembrane force probe, to measure the single-bond dissociation in three receptor-ligand… More >
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