Amine Batbat^1,2, Mohamed El Fadili³, Naoufal El Hachlafi^4,*, Amine Elbouzidi^5,*, Chaymae Hmimen⁶, Hiba Yahyaoui^2,7, Samir Jeddi⁶, Abdellatif Benbouazza², Kawtar Fikri-Benbrahim⁶, Mohamed Addi⁵, Samiah Hamad Al-Mijalli⁸, Khaoula Habbadi², Hassane Greche¹

Phyton-International Journal of Experimental Botany, Vol.94, No.2, pp. 481-501, 2025, DOI:10.32604/phyton.2025.059841 - 06 March 2025

Abstract This study highlights the regulatory potential antibacterial and antiradical of Origanum elongatum essential oil (EO), an endemic medicinal plant of Morocco used for its various properties. The chemical composition of the EO was characterized using gas chromatography-mass spectrometry (GC-MS). The antibacterial activity against different agricultural phytopathogens was determined by disc diffusion and microatmosphere methods, as well as by the determination of minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC), while the antioxidant activity was evaluated by DPPH and FRAP assays. To complement the experimental analyses, a molecular docking approach was used to predict and elucidate… More >

Samiah Hamad Al-Mijalli¹, Hanae Naceiri Mrabti², Amine Elbouzidi^3,*, Naglaa S. Ashmawy^4,5, Amine Batbat⁶, Emad M. Abdallah⁷, Wafa Laaboudi⁸, Mohammed Aladhadh⁹, Fahad M. Alshabrmi¹⁰, Sulaiman Mohammed Alnasser¹¹, Mohamed Addi³, Naoufal El Hachlafi¹²

Phyton-International Journal of Experimental Botany, Vol.94, No.1, pp. 209-227, 2025, DOI:10.32604/phyton.2025.060438 - 24 January 2025

Abstract Thymus serpyllum L., often known as wild thyme, has been used since ancient times due to its multifaceted culinary and medicinal attributes. It is usually utilized in folk medicine to manage different health issues. This work aimed to investigate the chemical composition and biological characteristics of T. serpyllum essential oil (EO), including its antimicrobial, antioxidant, and anti-inflammatory capabilities. Moreover, we have prompted an in-silico simulation to reveal the underlying mode of action of these properties. The chemical characterization of T. serpyllum (EO) by Gas Chromatography-Mass Spectrometry (GC-MS) indicated sabinene (17.33%), terpinen-4-ol (11.73%), phellandral (13.18%), and thymol (10.54%) as main… More >

Fayez Althobaiti¹, Hadeer Darwish^1,*, Raghad Alruqayb¹, Saqer S. Alotaibi¹, Fahad E. Alharthi², Ibrahim Jafri¹, Sarah Awwadh Altalhi¹, Ghadi Alsharif^3,4, Hussam Awwadh E Althagafi⁵, Hesham Noureldeen⁶, Sarah Alharthi⁷, Jawaher Albaqami², Najla Amin T. Al Kashgry², Ahmed Noureldeen²

Phyton-International Journal of Experimental Botany, Vol.94, No.1, pp. 181-198, 2025, DOI:10.32604/phyton.2025.059387 - 24 January 2025

Abstract The interest in using the Datura stramonium plant is due to its natural products, which are used in many pharmaceutical industries. The objective of the current study was to assess the therapeutic and cytotoxic effects of the D. stramonium plant on two types of human cancer cell models (MCF7 and HT29) in vitro. A soxhlet apparatus was used to obtain methanolic extract from dried plant leaves. The recovered crude, after the solvent had evaporated, was then dispersed at varied concentrations of extract 100, 50, 20, and 0.0 µg/mL and tested to see how the cells responded. Also,… More >

Yinyu Xu¹, Zheng Li¹, Qian Wen², Hao He¹, Pengtu Shi¹, Wenhua Zhou^2,*

Phyton-International Journal of Experimental Botany, Vol.93, No.9, pp. 2367-2382, 2024, DOI:10.32604/phyton.2024.047949 - 30 September 2024

Abstract The diversity of metabolites produced in fermented pine needles at different stages of fermentation has rarely been investigated. In the present study, untargeted metabolomic analysis using GC-MS and LC-MS was performed to detect metabolites in fermented pine needles at different fermentation stages. A total of 30 samples of pine needles fermented first with yeast (Saccharomyces cerevisiae) and then with a mixed bacterial culture of Lactobacillus fermentum CECT5716 and Bifidobacterium Breve M16V, were used to detect differential metabolites at different stages of fermentation. Pearson’s correlation analysis was used to determine correlations between metabolites and key microbial communities. A… More >

Ayda Khadhri¹, Mohamed Mendili¹, Marwa Bannour-Scharinger¹, Eric Masson², Antonio Pizzi^2,*

Journal of Renewable Materials, Vol.12, No.4, pp. 827-842, 2024, DOI:10.32604/jrm.2024.046950 - 12 June 2024

Abstract This study is the first to evaluate the phytochemical content and biological properties of Tunisian T. platyphyllos Scop. A total of 23 compounds of essential oils were identified by gas chromatography-mass spectrometry (GC-MS) analysis of bracts and fruit extracts. The results show that oxygenated monoterpenes were the dominant class of essential oils. The phenolic composition was investigated by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF). The analysis showed that the chemical profiles of the ethanolic extracts of bracts and fruits are substantially similar. The highest polyphenol content was found in the ethanolic extracts of the fruits (7.65… More > Graphic Abstract

Suzana Vieira Rabêlo¹, Fernanda Granja da Silva Oliveira², Michele Miriam Calixto de Lira³, Livia Macedo Dutra³, Adilson Sartoratto⁴, Marta Cristina Teixeira Duarte⁴, Maria Claudia dos Santos Luciano⁵, Maria Francilene Souza Silva⁵, Cláudia do Ó Pessoa⁵, Manoel Odorico de Moraes Filho⁵, Emmanoel Vilaça Costa⁶, Jackson Roberto Guedes da Silva Almeida^1,3,*

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 921-931, 2021, DOI:10.32604/phyton.2021.013305 - 30 March 2021

Abstract This study aimed to identify the chemical composition of essential oil from fruits (EOAF) and the hexanic crude extract from aerial parts (At-Hex) of atemoya (Annona cherimola x Annona squamosa), a hybrid belonging to the Annonaceae family. Cytotoxic and antimicrobial activity was also evaluated. OEAF was obtained by hydrodistillation using a Clevenger apparatus, and their composition was analyzed by gas chromatography-mass spectrometry (GC-MS) analyses. Cytotoxicity was tested against human tumor cell lines HCT-116 (colon carcinoma), SF-295 (glioblastoma), OVCAR-8 (ovarian carcinoma) and HL60 (leukemia) using 3-(4,5-dimethyl-2-thiazolyl)-2,5- diphenyl-2H-tetrazolium bromide (MTT) assay, while antimicrobial activity was conducted by bioauthography method More >

Chenggang Liang^1,#, Chunyu Wei^1,#, Yan Wang^1,*, Wujuan Yu¹, Kai Liao¹, Taoxiong Shi¹, Dabing Xiang^2,3, Qijiao Chen¹

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 871-893, 2021, DOI:10.32604/phyton.2021.014242 - 30 March 2021

Abstract Rice-Tartary buckwheat (RTB) is a special germplasm of Tartary buckwheat. In this study, the appraisal of taste quality between RTB and conventional Tartary buckwheat (CTB) was presented, and the metabolites in kernels at three typical grain filling stage (GFS) were investigated. Unlike CTB, RTB showed thin shell seeds without longitudinal furrows at maturity, which was easily artificially dehulled. Sense organ test indicated that RTB exhibited better taste quality because of the higher values of appearance, viscosity, taste and summary were appraised. In total, 92 metabolites were identified in kernels using GM-MS metabolomics platform. The levels… More >

Feng Liu^1,2,3, Zilan Ma^1,2,3, Marcos A. Caraballo-Ortiz⁴, Hui Zhang⁵, Xu Su^1,2,3, Yuping Liu^1,2,3,*

Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 1083-1090, 2020, DOI:10.32604/phyton.2020.011239 - 09 November 2020

Abstract Pedicularis longiflora var. tubiformis (Orobanchaceae) is an abundant parasitic herb mainly found in the Xiaopohu wetland of the Qinghai Lake Basin in Northwestern China. The species has an important local medicinal value, and in this study, we evaluated the chemical profile of its stems, leaves and seeds using mass spectrometry. Dried samples of stems, leaves and seeds were grinded, weighted, and used for a series of extractions with an ultrasonic device at room temperature. The chemical profiles for each tissue were determined using Gas Chromatography-Mass Spectrometry (GC-MS) and Liquid ChromatographyMass Spectrometry (LC-MS). Twenty-seven amino acids and organic… More >

Zhu XJ^†, YF Hu^†, X Chen, YH Wang, WP Fang, XH Li

Phyton-International Journal of Experimental Botany, Vol.83, pp. 57-63, 2014, DOI:10.32604/phyton.2014.83.057

Abstract The purpose of this study was to evaluate the antagonistic activity of two endophytic fungal strains, Pseudocercospora kaki and Penicillium sclerotiorum, isolated from the leaves of Camellia sinensis, against the rice blast pathogen Magnaporthe grisea. The inhibitory activity of the two endophytes against M. grisea in dual-culture was compared with that in monoculture. It was confirmed that the broth and its ethyl acetate extract of the dual-culture had a much stronger inhibition activity against M. grisea than the monocultures of P. kaki and P. sclerotiorum. The antagonism index of the broth and ethyl acetate extract from dual-culture to the mycelial growth of M. grisea… More >

Montenegro G^1,*, M Gómez¹, G Casaubon², A Belancic², AM Mujica¹, RC Peña¹

Phyton-International Journal of Experimental Botany, Vol.78, pp. 61-65, 2009, DOI:10.32604/phyton.2009.78.067

Abstract Three unifloral honeys were identified by the pronounced presence of specific compounds by means of sensorial analysis and SPME-GC-MS. Smoky and resinous (‘propolis’) odors characterized unifloral “quillay” (Quillaja saponaria) honey. “Corontillo” (Escallonia pulverulenta) honey was characterized by caramel and vanillin aromas, and “ulmo” (Eucryphia cordifolia) honey by having an anise scent with a floral jasmine note. Safranal was a useful marker for “corontillo” honey. Isophorone and cetoisophorone were the distinctive compounds of unifloral “ulmo” honey. In “quillay” honeys, megastigmatrienone, 2-p-hydroxyphenylalcohol and minor quantities of β-pinene and linalool oxide were correlated with their sensory properties such as resinous. More >