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  • Open Access


    Comparative Study of Genetic Structure and Genetic Diversity between Wild and Cultivated Populations of Taxus cuspidata, Northeast China

    Dandan Wang, Xiaohong Li, Yanwen Zhang*

    Phyton-International Journal of Experimental Botany, Vol.93, No.2, pp. 355-369, 2024, DOI:10.32604/phyton.2024.047183

    Abstract Taxus cuspidata is a rare plant with important medicinal and ornamental value. Aiming at the obvious differences between wild and cultivated populations of T. cuspidata from Northeast China, a total of 61 samples, that is, 33 wild yews and 28 cultivated yews were used to analyze the differences and correlations of the kinship, genetic diversity, and genetic structure between them by specific length amplified fragment sequencing (SLAF-seq). Finally, 470725 polymorphic SLAF tags and 58622 valid SNP markers were obtained. Phylogenetic analysis showed that 61 samples were classified into 2 clusters: wild populations and cultivated populations, and some wild yews were… More >

  • Open Access


    Genetic Diversity, Population Structure, and Genome-Wide Association Study of Seven Agronomic Traits in 273 Diverse Upload Cotton Accessions

    Yajun Liang1,2,#, Juyun Zheng1,#, Junduo Wang1,#, Zhaolong Gong1, Zhiqiang Li3, Ling Min4, Zeliang Zhang2, Zhiwei Sang2, Yanying Qu2, Xueyuan Li1,*, Quanjia Chen2,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.12, pp. 3345-3357, 2023, DOI:10.32604/phyton.2023.028755

    Abstract Upland cotton (Gossypium hirsutum) is the most important plant producing natural fibers for the textile industry. In this study, we first investigated the phenotypic variation of seven agronomic traits of 273 diverse cotton accessions in the years 2017 and 2018, which were from 18 geographical regions. We found large variations among the traits in different geographical regions and only half of the traits in either years 2017 or 2018 followed a normal distribution. We then genotyped the collection with 81,612 high quality SNPs. Phylogenetic tree and population structure revealed a diverse genetic structure of the core collection, and geographical diversification… More >

  • Open Access


    Estimation of Genetic Divergence and Character Association Studies in Local and Exotic Diversity Panels of Soybean (Glycine max L.) Genotypes

    Syed Ali Zafar1,*, Muhammad Aslam2, Haroon Zaman Khan3, Sehrish Sarwar1, Rao Saad Rehman4, Mariam Hassan1, Ramala Masood Ahmad2, Rafaqat A. Gill5, Basharat Ali6, Ibrahim Al-Ashkar7, Abdullah Ibrahim7, Md Atikur Rahman8, Ayman El Sabagh9,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1887-1906, 2023, DOI:10.32604/phyton.2023.027679

    Abstract The availability of favorable genetic diversity is a thriving vitality for the success of a breeding program. It provides a firm basis of selecting superior breeding lines for the development of high yielding crop genotypes. In this context, present investigation aimed to generate information on genetic divergence and character association in a diversity panel of 123 local and exotic soybean genotypes. Analysis of variance revealed significant response of the evaluated genotypes based on studied attributes. It depicted the probability of selecting desirable soybean genotypes by focusing on character association studies and genetic diversity analysis. Correlation analysis revealed that seed yield… More >

  • Open Access


    Pre-Breeding Genetic Diversity Assessment of Tomato (Solanum lycopersicum L.) Cultivars Based on Molecular, Morphological and Physicochemical Parameters

    Jameel M. Al-Khayri1,*, Salha M. Alshamrani2, Adel A. Rezk1, Wael F. Shehata1, Mustafa I. Almaghasla3, Tarek A. Shalaby3,4, Ahmed M. Saad5, Fatmah A. Safhi6, Muhammad N. Sattar7, Arafat A. Abdel Latef8, Mahmoud Z. Sitohy5, Abdallah A. Hassanin9,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.5, pp. 1493-1512, 2023, DOI:10.32604/phyton.2023.027375

    Abstract Appropriate knowledge of the parental cultivars is a pre-requisite for a successful breeding program. This study characterized fruit yield, quality attributes, and molecular variations of ten tomato cultivars during three consecutive generations under greenhouse conditions. Peto 86, Castle Rock, and Red Star cultivars showed the highest fruit yield (kg/plant), total phenolic compounds (TPC), and sap acidity. Principal component analysis categorized the evaluated fruit yield into three groups based on their quality attributes. A robust positive correlation appeared among traits inside each group. A positive correlation was likewise noticed between the first and the second groups. However, a negative correlation was… More >

  • Open Access


    Genetic Diversity and Population Structure Analysis of Barley Landraces from Shanghai Region Using Genotyping-by-Sequencing

    Luli Li1,2, Nigel G. Halford3, Huihui Wang4, Yingjie Zong1, Zhenzhu Guo1, Ruiju Lu1, Chenghong Liu1, Zhiwei Chen1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1275-1287, 2023, DOI:10.32604/phyton.2023.026946

    Abstract Barley (Hordeum vulgare L.) is an important economic crop for food, feed and industrial raw materials. In the present research, 112 barley landraces from the Shanghai region were genotyped using genotyping-by-sequencing (GBS), and the genetic diversity and population structure were analyzed. The results showed that 210,268 Single Nucleotide Polymorphisms (SNPs) were present in total, and the average poly-morphism information content (PIC) was 0.1642. Genetic diversity and population structure analyses suggested that these barley landraces were differentiated and could be divided into three sub-groups, with morphological traits of row-type and adherence of the hulls the main distinguishing factors between groups. Genotypes… More >

  • Open Access


    Molecular characterization of sweet potato (Ipomoea batatas [L.] Lam) germplasms for desirable traits by using simple sequence repeats markers


    BIOCELL, Vol.47, No.1, pp. 227-237, 2023, DOI:10.32604/biocell.2023.025135

    Abstract Every breeding program that aims to create new and improved cultivars with desired traits mostly relies on information related to genetic diversity. Therefore, molecular characterization of germplasms is important to obtain target cultivars with desirable traits. Sweet potato [Ipomoea batatas (L.) Lam] is widely considered the world’s most important crop, with great diversity in morphological and phenotypic traits. The genetic diversity of 20 sweet potato germplasms originating from Bangladesh, CIP, Philippines, Taiwan, and Malaysia were compared, which was accomplished by genetic diversity analysis by exploring 20 microsatellite DNA markers for germplasm characterization and utilization. This information was effective in differentiating… More >

  • Open Access


    Development of polymorphic SSR markers and their applicability in genetic diversity evaluation in Euptelea pleiosperma


    BIOCELL, Vol.46, No.11, pp. 2489-2495, 2022, DOI:10.32604/biocell.2022.020311

    Abstract Euptelea pleiosperma is a characteristic species of East Asian flora with both ornamental and scientific values. Based on the reduced-representation sequencing (RRS) technology of RAD-Seq, this study conducted high-throughput Illumina paired-end sequencing to find SSR marker information in the genome of E. pleiosperma, and to screen and verify polymorphism of SSR markers. We obtained 5.5G of high-quality data using RAD-Seq. The total number of contigs of the RAD tags was 299,376, with the maximum contig length of 2,062 bp and the average length of 445 bp. From these sequences, we identified 20,718 SSR loci, with a distribution density of one… More >

  • Open Access


    Diversity Assessment of an Endemic Carpinus oblongifolia (Betulaceae) Using Specific-Locus Amplified Fragment Sequencing and Implications for Conservation

    Sumei Li, Qing Wang, Peng Wang, Shuan Wang, Rutong Yang, Ya Li*

    Phyton-International Journal of Experimental Botany, Vol.91, No.3, pp. 617-632, 2022, DOI:10.32604/phyton.2022.017741

    Abstract Carpinus oblongifolia is an endemic species and the extant wild populations show a fragmentation distribution in the Baohua Mountain of Jiangsu Province in eastern China. Understanding of genetic diversity plays an important role in C. oblongifolia survival and sustainable development. The wild C. oblongifolia population was artificially divided into four subpopulations according to the microhabitats, and another two subpopulations were constructed by progeny seedlings cultivated with the mature seeds. Then, the leaf buds of 80 individuals from six subpopulations were sampled to develop single nucleotide polymorphisms (SNPs) using specific-locus amplified fragment sequencing (SLAF-seq). Based on these SNPs, we aimed to… More >

  • Open Access


    Genetic diversity in eggplant (Solanum melongena L.) germplasm from three secondary geographical origins of diversity using SSR markers


    BIOCELL, Vol.45, No.5, pp. 1393-1401, 2021, DOI:10.32604/biocell.2021.015321

    Abstract Indo-Burmese region was the primary center of eggplant diversity from where the crop extended to several secondary origins of diversity. In this study, the genetic diversity among fifty-six eggplant accessions collected from three countries was assessed using sixteen polymorphic SSR markers to determine suitable parents for heterotic hybridization. The estimation of genetic diversity among the population of three countries (Bangladesh, Malaysia, and Thailand) varied from 0.57 to 0.74, with Shannon’s index value of 0.65. The mean value of expected heterozygosity and Nei’s index was 0.49, with an average PIC value of 0.83. A dendrogram was constructed based on UPGMA (unweighted… More >

  • Open Access


    Development and characterization of Simple Sequence Repeat (SSR) markers from the genomic sequence of sweet potato [Ipomoea batatas L. (Lam)]


    BIOCELL, Vol.45, No.4, pp. 1095-1105, 2021, DOI:10.32604/biocell.2021.015053

    Abstract Sweet potato is a multifunctional root crop with many essential nutrients and bioactive compounds. Due to its genetic complexity and lack of genomic resources, efficient genetic studies and cultivar development lag far behind other major crops. Simple sequence repeats (SSRs) offer an effective molecular marker technology for molecular-based breeding and for locating important loci in crop plants, but only a few have previously been developed in sweet potato. To further explore new SSR markers and accelerate their use in sweet potato genetic studies, genome-wide characterization and development of SSR markers were performed using the recently published genome of sweet potato… More >

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