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  • Open Access

    ARTICLE

    Genetic Diversity of Tuberose (Polianthes tuberosa L.) Germplasm through Molecular Approaches to Obtain Desirable Plant Materials for Future Breeding Programs

    Vardhelli Soukya1, Soumen Maitra1,*, Nandita Sahana2, Saikat Das3, Rupsanatan Mandal3, Arpita Mandal Khan1, Arindam Das4, Ashok Choudhury5, Prodyut Kumar Paul6, Ahmed Gaber7, Mohammed M. Althaqafi8, Akbar Hossain9,*

    Phyton-International Journal of Experimental Botany, Vol.94, No.11, pp. 3493-3508, 2025, DOI:10.32604/phyton.2025.071450 - 01 December 2025

    Abstract The present study investigated the genetic diversity of 24 germplasms of Polianthes tuberosa L. via 16 inter simple sequence repeat (ISSR) marker techniques. The research findings revealed that the ISSR markers presented higher levels of band reproducibility and were more efficient at clustering germplasms. Among the 16 markers examined in this study, 12 had a complete polymorphism rate of 100%. The molecular analysis revealed a PIC ranging from 0.079 to 0.373, with a mean value of 0.30, whereas the range of the marker index was from 0.0001 to 0.409, with an average value of 0.03, and More >

  • Open Access

    ARTICLE

    Evaluation of Floral Phenotypic Diversity of Kiwifruit Male Germplasm Resources

    Yuqing Wan1, Lihua Wang2, Qian Zhang2, Qian Gao1, Xin Liu2, Gaomin Fan2, Yan Wan1, Xiaoqin Zheng1,*

    Phyton-International Journal of Experimental Botany, Vol.94, No.9, pp. 2781-2796, 2025, DOI:10.32604/phyton.2025.071151 - 30 September 2025

    Abstract Exploring the phenotypic trait variation and diversity of kiwifruit male plant resources can support selection, breeding, and genetic improvement, ultimately enhancing agricultural production. In this study, 50 kiwifruit male plants were collected from the resource nursery of Sichuan Provincial Natural Resources Bureau. The phenotypic variation of the germplasm was analyzed using 16 quantitative traits. The analysis involved coefficient of variation (CV), Shannon-Wiener index (H), principal component analysis, correlation analysis, cluster analysis, and comprehensive evaluation. The results showed that the variation range of 16 phenotypic traits in kiwifruit male germplasm resources was 1.55% to 83.71%, with… More >

  • Open Access

    ARTICLE

    Significant Changes in Morphological Traits of 422 Barley (Hordeum vulgare L.) Varieties with Different Registration

    Valentina Spanic1,*, Zvonimir Lalic2, Ivica Berakovic1, Luka Drenjancevic2, Goran Jukic2, Ivan Varnica2

    Phyton-International Journal of Experimental Botany, Vol.94, No.2, pp. 317-330, 2025, DOI:10.32604/phyton.2025.058201 - 06 March 2025

    Abstract Enhanced grain yield is achieved in barley by developing varieties incorporating grain yield-related and morphological traits derived from different varieties. The evaluation of 28 morphological characteristics of 422 barley varieties was carried out to assess their changes over time from 1973 to 2023. Most barley yield improvement seems to have been achieved by changes in morphological traits where modern varieties out-yielded older varieties for more than 30% (from 1973 to 2023). According to the Pareto chart, the length of the first segment of the rachis was found to be the most important parameter that changed… More >

  • Open Access

    ARTICLE

    Characterization and Genetic Diversity of Tea (Camellia sinensis (L.) O. Kuntze) Genotypes for Waterlogging Tolerance

    Md. Riyadh Arefin1,2, Md. Sabibul Haque1, A. K. M. Golam Sarwar1,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.12, pp. 3411-3442, 2024, DOI:10.32604/phyton.2024.058893 - 31 December 2024

    Abstract Tea estates in Bangladesh are currently experiencing frequent waterlogging stress due to heavy rainfall or flooding under changing climate scenarios. The development of waterlogging-tolerant tea cultivars is, therefore, crucial for sustainable tea production with increased yield in waterlogging-affected areas. In this study, a total of two hundred tea genotypes were screened for waterlogging tolerance based on yield and quality potentials. Tea genotypes were raised at the nursery and evaluated with 13 traits at the seedling and mature stages. Descriptive and multivariate analysis of the recorded data of measured traits were performed for clustering the… More >

  • Open Access

    ARTICLE

    Genetic Diversity and Combining Ability of Developed Maize Lines to Realize Heterotic and High Yielding Hybrids for Arid Conditions

    Mohamed M. Kamara1, Fatmah A. Safhi2, Nora M. Al Aboud3, Maha Aljabri3, Samah A. Alharbi3, Hesham S. Ghazzawy4,5, Mohammed O. Alshaharni6, Eman Fayad7, Wessam F. Felemban8,9, Diaa Abd El-Moneim10, Abdallah A. Hassanin11, Imen Ben Abdelmalek12,*, Abdelraouf M. Ali13, Elsayed Mansour14,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.12, pp. 3465-3485, 2024, DOI:10.32604/phyton.2024.058628 - 31 December 2024

    Abstract Developing high-yield maize hybrids is critical for sustaining maize production, especially in the face of rapid climate changes and the growing global population. Exploring the genetic diversity and combining ability in parental inbreds is needed for developing such high-yielding hybrids. Consequently, this study aimed at evaluating parental genetic diversity employing simple sequence repeats (SSR) markers, estimating effects of general (GCA) and specific (SCA) combining abilities for grain yield and yield contributing characters, identifying high yielding hybrids, and evaluating the association of SCA effects and performance of hybrids with genetic distance. Half-diallel mating scheme was utilized… More >

  • Open Access

    ARTICLE

    Comparative Study of Genetic Structure and Genetic Diversity between Wild and Cultivated Populations of Taxus cuspidata, Northeast China

    Dandan Wang, Xiaohong Li, Yanwen Zhang*

    Phyton-International Journal of Experimental Botany, Vol.93, No.2, pp. 355-369, 2024, DOI:10.32604/phyton.2024.047183 - 27 February 2024

    Abstract Taxus cuspidata is a rare plant with important medicinal and ornamental value. Aiming at the obvious differences between wild and cultivated populations of T. cuspidata from Northeast China, a total of 61 samples, that is, 33 wild yews and 28 cultivated yews were used to analyze the differences and correlations of the kinship, genetic diversity, and genetic structure between them by specific length amplified fragment sequencing (SLAF-seq). Finally, 470725 polymorphic SLAF tags and 58622 valid SNP markers were obtained. Phylogenetic analysis showed that 61 samples were classified into 2 clusters: wild populations and cultivated populations, and some… More >

  • Open Access

    ARTICLE

    Genetic Diversity, Population Structure, and Genome-Wide Association Study of Seven Agronomic Traits in 273 Diverse Upload Cotton Accessions

    Yajun Liang1,2,#, Juyun Zheng1,#, Junduo Wang1,#, Zhaolong Gong1, Zhiqiang Li3, Ling Min4, Zeliang Zhang2, Zhiwei Sang2, Yanying Qu2, Xueyuan Li1,*, Quanjia Chen2,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.12, pp. 3345-3357, 2023, DOI:10.32604/phyton.2023.028755 - 28 December 2023

    Abstract Upland cotton (Gossypium hirsutum) is the most important plant producing natural fibers for the textile industry. In this study, we first investigated the phenotypic variation of seven agronomic traits of 273 diverse cotton accessions in the years 2017 and 2018, which were from 18 geographical regions. We found large variations among the traits in different geographical regions and only half of the traits in either years 2017 or 2018 followed a normal distribution. We then genotyped the collection with 81,612 high quality SNPs. Phylogenetic tree and population structure revealed a diverse genetic structure of the core… More >

  • Open Access

    ARTICLE

    Estimation of Genetic Divergence and Character Association Studies in Local and Exotic Diversity Panels of Soybean (Glycine max L.) Genotypes

    Syed Ali Zafar1,*, Muhammad Aslam2, Haroon Zaman Khan3, Sehrish Sarwar1, Rao Saad Rehman4, Mariam Hassan1, Ramala Masood Ahmad2, Rafaqat A. Gill5, Basharat Ali6, Ibrahim Al-Ashkar7, Abdullah Ibrahim7, Md Atikur Rahman8, Ayman El Sabagh9,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1887-1906, 2023, DOI:10.32604/phyton.2023.027679 - 11 April 2023

    Abstract The availability of favorable genetic diversity is a thriving vitality for the success of a breeding program. It provides a firm basis of selecting superior breeding lines for the development of high yielding crop genotypes. In this context, present investigation aimed to generate information on genetic divergence and character association in a diversity panel of 123 local and exotic soybean genotypes. Analysis of variance revealed significant response of the evaluated genotypes based on studied attributes. It depicted the probability of selecting desirable soybean genotypes by focusing on character association studies and genetic diversity analysis. Correlation… More >

  • Open Access

    ARTICLE

    Pre-Breeding Genetic Diversity Assessment of Tomato (Solanum lycopersicum L.) Cultivars Based on Molecular, Morphological and Physicochemical Parameters

    Jameel M. Al-Khayri1,*, Salha M. Alshamrani2, Adel A. Rezk1, Wael F. Shehata1, Mustafa I. Almaghasla3, Tarek A. Shalaby3,4, Ahmed M. Saad5, Fatmah A. Safhi6, Muhammad N. Sattar7, Arafat A. Abdel Latef8, Mahmoud Z. Sitohy5, Abdallah A. Hassanin9,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.5, pp. 1493-1512, 2023, DOI:10.32604/phyton.2023.027375 - 09 March 2023

    Abstract Appropriate knowledge of the parental cultivars is a pre-requisite for a successful breeding program. This study characterized fruit yield, quality attributes, and molecular variations of ten tomato cultivars during three consecutive generations under greenhouse conditions. Peto 86, Castle Rock, and Red Star cultivars showed the highest fruit yield (kg/plant), total phenolic compounds (TPC), and sap acidity. Principal component analysis categorized the evaluated fruit yield into three groups based on their quality attributes. A robust positive correlation appeared among traits inside each group. A positive correlation was likewise noticed between the first and the second groups.… More >

  • Open Access

    ARTICLE

    Genetic Diversity and Population Structure Analysis of Barley Landraces from Shanghai Region Using Genotyping-by-Sequencing

    Luli Li1,2, Nigel G. Halford3, Huihui Wang4, Yingjie Zong1, Zhenzhu Guo1, Ruiju Lu1, Chenghong Liu1, Zhiwei Chen1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1275-1287, 2023, DOI:10.32604/phyton.2023.026946 - 06 January 2023

    Abstract Barley (Hordeum vulgare L.) is an important economic crop for food, feed and industrial raw materials. In the present research, 112 barley landraces from the Shanghai region were genotyped using genotyping-by-sequencing (GBS), and the genetic diversity and population structure were analyzed. The results showed that 210,268 Single Nucleotide Polymorphisms (SNPs) were present in total, and the average poly-morphism information content (PIC) was 0.1642. Genetic diversity and population structure analyses suggested that these barley landraces were differentiated and could be divided into three sub-groups, with morphological traits of row-type and adherence of the hulls the main distinguishing More >

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