KHANDAKAR ABU MD MOSTAFIZAR RAHMAN^1,2, ABDUL SHUKOR JURAIMI^2,*, MD. REZWAN MOLLA³, MUHAMMAD ASYRAF MD HATTA⁴, ZULKEFLY BIN SULAIMAN⁵, SHAMIMA SULTANA⁶, AHMED GABER⁷, BENUKAR BISWAS⁸, AKBAR HOSSAIN^9,*

BIOCELL, Vol.47, No.1, pp. 227-237, 2023, DOI:10.32604/biocell.2023.025135 - 26 September 2022

Abstract Every breeding program that aims to create new and improved cultivars with desired traits mostly relies on information related to genetic diversity. Therefore, molecular characterization of germplasms is important to obtain target cultivars with desirable traits. Sweet potato [Ipomoea batatas (L.) Lam] is widely considered the world’s most important crop, with great diversity in morphological and phenotypic traits. The genetic diversity of 20 sweet potato germplasms originating from Bangladesh, CIP, Philippines, Taiwan, and Malaysia were compared, which was accomplished by genetic diversity analysis by exploring 20 microsatellite DNA markers for germplasm characterization and utilization. This information… More >

XIAOJUN ZHOU^*, XIAOYU LU, XUBO WANG

BIOCELL, Vol.46, No.11, pp. 2489-2495, 2022, DOI:10.32604/biocell.2022.020311 - 07 July 2022

Abstract Euptelea pleiosperma is a characteristic species of East Asian flora with both ornamental and scientific values. Based on the reduced-representation sequencing (RRS) technology of RAD-Seq, this study conducted high-throughput Illumina paired-end sequencing to find SSR marker information in the genome of E. pleiosperma, and to screen and verify polymorphism of SSR markers. We obtained 5.5G of high-quality data using RAD-Seq. The total number of contigs of the RAD tags was 299,376, with the maximum contig length of 2,062 bp and the average length of 445 bp. From these sequences, we identified 20,718 SSR loci, with a distribution… More >

Sumei Li, Qing Wang, Peng Wang, Shuan Wang, Rutong Yang, Ya Li^*

Phyton-International Journal of Experimental Botany, Vol.91, No.3, pp. 617-632, 2022, DOI:10.32604/phyton.2022.017741 - 26 October 2021

Abstract Carpinus oblongifolia is an endemic species and the extant wild populations show a fragmentation distribution in the Baohua Mountain of Jiangsu Province in eastern China. Understanding of genetic diversity plays an important role in C. oblongifolia survival and sustainable development. The wild C. oblongifolia population was artificially divided into four subpopulations according to the microhabitats, and another two subpopulations were constructed by progeny seedlings cultivated with the mature seeds. Then, the leaf buds of 80 individuals from six subpopulations were sampled to develop single nucleotide polymorphisms (SNPs) using specific-locus amplified fragment sequencing (SLAF-seq). Based on these SNPs, we… More >

DEBI RANI DATTA¹, MOHD RAFII YUSOP^1,2,*, AZIZAH MISRAN², MASHITAH JUSOH², YUSUFF OLADOSU¹, FATAI AROLU¹, AZADUL HAQUE¹, NADZIRAH M. SULAIMAN¹

BIOCELL, Vol.45, No.5, pp. 1393-1401, 2021, DOI:10.32604/biocell.2021.015321 - 12 July 2021

Abstract Indo-Burmese region was the primary center of eggplant diversity from where the crop extended to several secondary origins of diversity. In this study, the genetic diversity among fifty-six eggplant accessions collected from three countries was assessed using sixteen polymorphic SSR markers to determine suitable parents for heterotic hybridization. The estimation of genetic diversity among the population of three countries (Bangladesh, Malaysia, and Thailand) varied from 0.57 to 0.74, with Shannon’s index value of 0.65. The mean value of expected heterozygosity and Nei’s index was 0.49, with an average PIC value of 0.83. A dendrogram was… More >

HANNA AMOANIMAA-DEDE, JIACHENG ZHANG, CHUNTAO SU, HONGBO ZHU^*

BIOCELL, Vol.45, No.4, pp. 1095-1105, 2021, DOI:10.32604/biocell.2021.015053 - 22 April 2021

Abstract Sweet potato is a multifunctional root crop with many essential nutrients and bioactive compounds. Due to its genetic complexity and lack of genomic resources, efficient genetic studies and cultivar development lag far behind other major crops. Simple sequence repeats (SSRs) offer an effective molecular marker technology for molecular-based breeding and for locating important loci in crop plants, but only a few have previously been developed in sweet potato. To further explore new SSR markers and accelerate their use in sweet potato genetic studies, genome-wide characterization and development of SSR markers were performed using the recently… More >

MADE DIOUF¹, SARA DIALLO¹, FRANÇOIS ABAYE BADIANE^1,2, OUMAR DIACK¹, DIAGA DIOUF^1,*

BIOCELL, Vol.45, No.2, pp. 345-362, 2021, DOI:10.32604/biocell.2021.013706 - 19 February 2021

Abstract Cowpea is one of the most important legume grains in the sub-Saharan region of Africa used for human consumption and animal feed, but its production is hampered by biotic and abiotic constraints raising the need to broaden its genetic basis. For this purpose, seeds of two cowpea varieties Melakh and Yacine were irradiated with 300 and 340 Gy of gamma-ray, respectively. The developed mutant populations were agromorphologically characterized from M5 to M7, while the genetic diversity of the latter was evaluated using 13 ISSR markers. Based on the agromorphological characterization, variation of flower color, pod… More >

Riaz Ahmad¹, Muhammad Akbar Anjum^1,*, Safina Naz¹, Rashad Mukhtar Balal²

Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 17-34, 2021, DOI:10.32604/phyton.2020.011680 - 20 November 2020

Abstract Selection and use of molecular markers for evaluation of DNA polymorphism in plants are couple of the most important approaches in the field of molecular genetics. The assessment of genetic diversity using morphological markers is not sufficient due to little differentiating traits among the species, genera or their individuals. Morphological markers are not only highly influenced by environmental factors but skilled assessment is also prerequisite to find the variations in plant genetic resources. Therefore, molecular markers are considered as efficient tools for detailed DNA based characterization of fruit crops. Molecular markers provide new directions to More >

ALI RAZA^1,7,#,*, ABU BAKR UMER FAROOQ^2,#, WAQAR AHMAD KHAN³, AHSAN IQBAL⁴, SADETTIN ÇELİK⁵, MAHWISH ALI⁶, RAO SOHAIL AHMAD KHAN^1,*

BIOCELL, Vol.44, No.4, pp. 769-776, 2020, DOI:10.32604/biocell.2020.010207 - 24 December 2020

Abstract Randomly amplified polymorphic DNA (RAPD) is a tremendously convenient approach used to discriminate between Brassica species owing to its accuracy and speed. RAPD primers generate adequate genetic information that can be used in the primer-marker system. In this work, twenty RAPD-PCR based markers were executed to generate polymorphic data, like polymorphic information content (PIC), mean resolving power (MRP), resolving power (RP), effective multiplex ratio (EMR), and marker index (MI) for the first time and genetic distance among and between six Brassica species were calculated. Our results indicated that 20 primers produced a total of 231 scored… More >

TING YANG^#, JIANNONG LU^#, AKWASI YEBOAH, SHUAILEI GU, DONGNA LI, YUZHEN SHI, XUEGUI YIN^*

BIOCELL, Vol.44, No.3, pp. 381-388, 2020, DOI:10.32604/biocell.2020.09112 - 22 September 2020

Abstract In order to provide a molecular basis for selecting good hybrid combinations for the identification of castor bean germplasm resources, fingerprint and genetic diversity analysis of 52 castor bean materials from 12 regions in 5 countries were constructed by using the Functinal Markers (FMs) associated with fatty acid metabolism-related genes. A total of 72 alleles were amplified by 29 pairs of FMs with an average of 2.483 per marker and the polymorphic information content was 0.103–0.695. Shannon’s information index (I), observed heterozygosity (Ho) and expected heterozygosity (He) were 0.699, 0.188 and 0.436 respectively. The clustering More >

Tantasawat PA, K Lunwongsa, T Linthaisong, P Wirikitgul, N Campatong, N Talpolkrung, A Tharapreuksapong, O Poolsawat, A Khairum, A Sorntip, C Kativat

Phyton-International Journal of Experimental Botany, Vol.87, pp. 162-170, 2018, DOI:10.32604/phyton.2018.87.162

Abstract Genetic diversity and relatedness of 17 water primrose (Ludwigia hyssopifolia) accessions in Thailand were estimated using morphological characters and random amplified polymorphic DNA (RAPD) markers. Eight morphological characters were diverse among the accessions. However, some accessions could not be distinguished from one another based on these morphological characters alone. Unweighted pair-group arithmetic average (UPGMA) analysis of these characters separated these 17 accessions into 2 major clusters. Among the 5 RAPD primers used, a total of 68 fragments (150 to 2000 bp) were amplified, showing a polymorphism percentage of 80%. The polymorphic information content (PIC) among accessions… More >