MADE DIOUF¹, SARA DIALLO¹, FRANÇOIS ABAYE BADIANE^1,2, OUMAR DIACK¹, DIAGA DIOUF^1,*

BIOCELL, Vol.45, No.2, pp. 345-362, 2021, DOI: 10.32604/biocell.2021.013706

Abstract Cowpea is one of the most important legume grains in the sub-Saharan region of Africa used for human consumption and animal feed, but its production is hampered by biotic and abiotic constraints raising the need to broaden its genetic basis. For this purpose, seeds of two cowpea varieties Melakh and Yacine were irradiated with 300 and 340 Gy of gamma-ray, respectively. The developed mutant populations were agromorphologically characterized from M5 to M7, while the genetic diversity of the latter was evaluated using 13 ISSR markers. Based on the agromorphological characterization, variation of flower color, pod length, seed coat color, and… More >

ALI RAZA^1,7,#,*, ABU BAKR UMER FAROOQ^2,#, WAQAR AHMAD KHAN³, AHSAN IQBAL⁴, SADETTIN ÇELİK⁵, MAHWISH ALI⁶, RAO SOHAIL AHMAD KHAN^1,*

BIOCELL, Vol.44, No.4, pp. 769-776, 2020, DOI:10.32604/biocell.2020.010207

Abstract Randomly amplified polymorphic DNA (RAPD) is a tremendously convenient approach used to discriminate between Brassica species owing to its accuracy and speed. RAPD primers generate adequate genetic information that can be used in the primer-marker system. In this work, twenty RAPD-PCR based markers were executed to generate polymorphic data, like polymorphic information content (PIC), mean resolving power (MRP), resolving power (RP), effective multiplex ratio (EMR), and marker index (MI) for the first time and genetic distance among and between six Brassica species were calculated. Our results indicated that 20 primers produced a total of 231 scored band and generated 87%… More >

Riaz Ahmad¹, Muhammad Akbar Anjum^1,*, Safina Naz¹, Rashad Mukhtar Balal²

Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 17-34, 2021, DOI:10.32604/phyton.2020.011680

Abstract Selection and use of molecular markers for evaluation of DNA polymorphism in plants are couple of the most important approaches in the field of molecular genetics. The assessment of genetic diversity using morphological markers is not sufficient due to little differentiating traits among the species, genera or their individuals. Morphological markers are not only highly influenced by environmental factors but skilled assessment is also prerequisite to find the variations in plant genetic resources. Therefore, molecular markers are considered as efficient tools for detailed DNA based characterization of fruit crops. Molecular markers provide new directions to the efforts of plant breeders… More >

TING YANG^#, JIANNONG LU^#, AKWASI YEBOAH, SHUAILEI GU, DONGNA LI, YUZHEN SHI, XUEGUI YIN^*

BIOCELL, Vol.44, No.3, pp. 381-388, 2020, DOI:10.32604/biocell.2020.09112

Abstract In order to provide a molecular basis for selecting good hybrid combinations for the identification of castor bean germplasm resources, fingerprint and genetic diversity analysis of 52 castor bean materials from 12 regions in 5 countries were constructed by using the Functinal Markers (FMs) associated with fatty acid metabolism-related genes. A total of 72 alleles were amplified by 29 pairs of FMs with an average of 2.483 per marker and the polymorphic information content was 0.103–0.695. Shannon’s information index (I), observed heterozygosity (Ho) and expected heterozygosity (He) were 0.699, 0.188 and 0.436 respectively. The clustering results indicated that the castor… More >

Valera-Montero LL, PJ Muñoz-Rodríguez, H Silos-Espino, S Flores-Benítez

Phyton-International Journal of Experimental Botany, Vol.85, pp. 176-183, 2016, DOI:10.32604/phyton.2016.85.176

Abstract Guava fruit produced in Calvillo, Aguascalientes (Mexico) is considered to be of the best quality in this country. Nevertheless, growers from this place empirically know that there is a noticeable variation among individual trees within the same orchard, and variation among individuals from different orchards. In order to have a clear evidence of this, morphology analysis of guava was performed taking data from seventy nine individuals out of thirty six orchards, while RAPD was performed on a subset of twenty six individuals. Similarity was found for morphology data ranging from 87-100%, while similarity from RAPD data ranged from 30- 100%.… More >

Olvera-Mendoza EI¹, SI Lara-Cabrera¹, C Sáenz-Romero², R Lindig-Cisneros³

Phyton-International Journal of Experimental Botany, Vol.85, pp. 169-175, 2016, DOI:10.32604/phyton.2016.85.169

Abstract To quantify the effect on genetic diversity of restoring tree populations in an area under the influence of an urban heat island, we evaluated the genetic diversity of 72 Ceiba aesculifolia individuals, from a restoration experiment established from 2170 to 2260 m a.s.l. Reintroduced individuals were compared with the provenance from which the seeds were obtained, and two external provenances, for a total of 123 individuals. Samples were analyzed with three AFLP primer combinations. Polymorphisms of 38.4 to 62.5% were obtained. Genetic diversity estimated with the Simpson index ranged from 0.14 to 0.2. The provenances in the restoration site had… More >

Martínez ME¹, JM Lesher G², G Castañón N², E de la Cruz L¹, C Zapata H²

Phyton-International Journal of Experimental Botany, Vol.82, pp. 209-214, 2013, DOI:10.32604/phyton.2013.82.209

Abstract Nanche (Byrsonima crassifolia (L.) H.B.K.) grows in the southeastern region of the Mexican republic under both wild and semi-cultivated forms. Despite the importance of the fruit of this tree, only one paper at the molecular level has been published in Mexico, and possibly in America, to date. For this reason, the purpose of this research was to study the genetic variability of nanche trees that grow under both wild and semi-cultivated conditions in the state of Tabasco, Mexico. Molecular determinations were carried out in the Laboratorio de Genómica of the División Académica de Ciencias Biológicas (DACBiológicas), Universidad Juárez Autónoma de… More >

Escalante-González JG¹, EA García-Zambrano², A Gutiérrez-Diez², RE Vázquez-Alvarado², JA Torres-Castillo², SR Sinagawa-García², F Zavala-García², J Martínez-de la Cerda², C Gallegos-Vázquez³, JI García-Zambrano⁴

Phyton-International Journal of Experimental Botany, Vol.81, pp. 143-148, 2012, DOI:10.32604/phyton.2012.81.143

Abstract The objective of this research was to increase the genetic diversity of the Nopal Germplasm Bank of the FAUANL using RAPD molecular markers to estimate the genetic diversity of 15 accessions of cactus pear from molecular markers recorded in the Bank. DNA extraction was performed by the method of cellular ruptor using FastDNA^® Kit. Performance measurement of DNA was carried out through Picoflour. It was observed that the DNA concentrations ranged from 74.91 ng/µL to 12.32 ng/uL. Twelve primers were used to generate from 1 to 5 bands per primer with a molecular weight ranging from 200 to 1000 bp.… More >

García-Zambrano² EA, F Zavala-García², A Gutiérrez-Diez, MC Ojeda-Zacarías, I Cerda-Hurtado³

Phyton-International Journal of Experimental Botany, Vol.78, pp. 117-120, 2009, DOI:10.32604/phyton.2009.78.117

Abstract The objective of this study was to apply the AFLP-type molecular marker technique to estimate genetic diversity in cactus pear within the Germplasm Bank of FAUANL. Twelve accessions have been reported as possible duplicates through type RAPD- molecular markers in such Bank. DNA was extracted using the cellular ruptor technique, and then digested and bound. A preamplification and subsequent selective amplification were conducted. Amplified fragments were then separated and analyzed. It was concluded that none of the accessions was duplicated. This was because 8 groups were formed in the dendogram obtained after applying the UPGMA method. There were 3 accessions… More >

Ochoa-Fuentes YM¹, E Cerna², J Landeros², O Vazquez¹, V Olalde³, A Flores²

Phyton-International Journal of Experimental Botany, Vol.78, pp. 25-30, 2009, DOI:10.32604/phyton.2009.78.025

Abstract Forty two Mexican isolates of Phytophthora cinnamomi Rands were examined for genetic diversity by the random amplified polymorphic DNA assay. The amount of genetic variation was evaluated by polymerase chain reaction amplification with a set of twenty one random 10-mer primers. All amplifications revealed scorable polymorphisms among the isolates, and a total of 23 band positions were scored for the 21 primers tested. Genetic distances among the isolates were calculated, and cluster analysis was used to generate a dendogram which showed relationships between them. These results suggest that the geographic groups were strong. More >