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  • Open Access

    ARTICLE

    Genome-Wide GRAS Gene Family Analysis Reveals the Classification, Expression Profiles in Melon (Cucumis melo L.)

    Yanfei Bi1, Bin Wei1, Ying Meng2, Zhongzhao Li2, Zhenghui Tang1, Feng Yin3, Chuntao Qian2,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1161-1175, 2021, DOI:10.32604/phyton.2021.014396

    Abstract Melon (Cucumis melo), belonging to the Cucurbitaceae family, is a globally important economic crop. GRAS (GAI, RGA, SCR) genes, which are a type of transcription factor, play a critical role in plant growth and development, including processes such as radial root patterning, light signalling, abiotic/biotic stress, axillary shoot meristem formation, and phytohormone (gibberellin) signal transduction. In this study, the GRAS family in melon was analysed comprehensively with respect to chromosomal location, motif prediction, gene structure, and expression pattern. A total of 37 GRAS genes were first identified in melon, after which a phylogenetic tree was built with the GRAS genes… More >

  • Open Access

    ARTICLE

    Genome-wide identification of WRKY gene family and expression analysis under abiotic stresses in Andrographis paniculata

    QICHAO WANG1, WUJING ZENG1, BASHARAT ALI2, XUEMIN ZHANG3, LING XU1,*, ZONGSUO LIANG1,*

    BIOCELL, Vol.45, No.4, pp. 1107-1119, 2021, DOI:10.32604/biocell.2021.015282

    Abstract Andrographis paniculata (A. paniculata) is a Chinese herbal medicine that clears away heat, reduces inflammation, protects the liver, and promotes choleretics. The WRKYs of A. paniculata are still not well characterized, although many WRKYs have been identified in various plant species. In the present study, 59 A. paniculata WRKY (ApWRKY) genes were identified and renamed on the basis of their respective chromosome distribution. These ApWRKYs were divided into three groups via phylogenetic analysis according to their WRKY domains and combined with WRKY of Arabidopsis. The 59 identified ApWRKY transcription factors were nonuniformity distributed on 23 chromosomes of A. paniculata. From… More >

  • Open Access

    ARTICLE

    Genome-wide identification and expression analysis of Aux/IAA gene family in strawberry (Fragaria vesca)

    LIYAN SU1,2,#, HONG YU3,#, JIUDONG ZHANG1, XIAOHONG ZHAO1, XUAN LIU1, QIANG LI1,4,*

    BIOCELL, Vol.45, No.4, pp. 1153-1166, 2021, DOI:10.32604/biocell.2021.014499

    Abstract Auxin signaling and its components (Auxin/Indole-3-Acetic Acid (Aux/IAA)) are critical for plant growth and development. Here, we performed a genome-wide annotation and identified twenty-one Aux/IAA genes in strawberry (Fragaria vesca). Most FveIAAs were located on chromosomes 1, 2, 4, 5, and 6, while no FveIAAs were found in chromosomes 3 and 7. Phylogenetic analysis divided these genes into nine subfamilies. Most FveIAAs contained the DNA-binding and Aux/IAA domains, as well as motifs I–IV. There were 2–6 exons in the FveIAA genes based on the gene structure analysis. Also, we found that four pairs of FveIAA genes underwent segment duplications. Moreover,… More >

  • Open Access

    ARTICLE

    Genome-Wide Identification of Candidate Genes Associated with β-glucan Traits in a Hulled and Hulless Barley (Hordeum vulgare L.) Population

    Longhua Zhou1,2,#, Ting He1,2,#, Guimei Guo1,2, Jing Li1,2, Ruiju Lu1,2, Yingbo Li1,2,*, Chenghong Liu1,2,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 987-997, 2020, DOI:10.32604/phyton.2020.011728

    Abstract Barley grain is a valuable source of β-glucan, which is an important component of dietary fiber with significant human health benefits. Although the genetic basis of β-glucan biosynthesis has been widely studied, a genome-wide association study (GWAS) is still required for a scan of the candidate genes related to the complex quantitative trait based on the high-quality barley reference genome. In this study, a GWAS was conducted using a population composed of 87 barley landraces (39 hulled and 48 hulless, β-glucan from 2.07% to 6.56%) with 191,098 nucleotide polymorphisms (SNPs) markers to cover the chromosomes with the highest density. The… More >

  • Open Access

    ARTICLE

    Genome-Wide Identification and Expression Profiling Suggest that Invertase Genes Function in Silique Development and the Response to Sclerotinia sclerotiorum in Brassica napus

    Jingsen Liu1,2, Jinqi Ma1,2, Ai Lin1,2, Chao Zhang1,2, Bo Yang1,2, Liyuan Zhang1,2, Lin Huang1,2, Jiana Li1,2,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 253-273, 2020, DOI:10.32604/phyton.2020.09334

    Abstract Invertase (INV), a key enzyme in sucrose metabolism, irreversibly catalyzes the hydrolysis of sucrose to glucose and fructose, thus playing important roles in plant growth, development, and biotic and abiotic stress responses. In this study, we identified 27 members of the BnaINV family in Brassica napus. We constructed a phylogenetic tree of the family and predicted the gene structures, conserved motifs, cis-acting elements in promoters, physicochemical properties of encoded proteins, and chromosomal distribution of the BnaINVs. We also analyzed the expression of the BnaINVs in different tissues and developmental stages in the B. napus cultivar Zhongshuang 11 using qRT-PCR. In… More >

  • Open Access

    ARTICLE

    Genome-Wide Identification of the F-box Gene Family and Expression Analysis under Drought and Salt Stress in Barley

    Lantian Zhang, Siyi Wang, Yuyu Chen, Mengyuan Dong, Yunxia Fang, Xian Zhang, Tao Tong, Ziling Zhang, Junjun Zheng, Dawei Xue*, Xiaoqin Zhang*

    Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 229-251, 2020, DOI:10.32604/phyton.2020.10022

    Abstract The F-box protein-encoding gene family plays an essential role in plant stress resistance. In present study, 126 non-redundant F-box genes were identified in barley (Hordeum vulgare L., Hv). The corresponding proteins contained 165– 887 amino acid residues and all were amphiphilic, except 5 proteins. Phylogenetic analysis of F-box protein sequences in barley and stress-related F-box protein sequences in wheat and Arabidopsis thaliana (At) was used to classify barley F-box genes are divided into 9 subfamilies (A–I). A structure-based sequence alignment demonstrated that F-box proteins were highly conserved with a total of 10 conserved motifs. In total, 124 F-box genes were… More >

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