JUAN LIN1, XUANWEI ZHOU2, JIONG FEI2, ZHIHUA LIAO1, WANG JIN1, XIAOFEN SUN1,*, KEXUAN TANG1,2,*
BIOCELL, Vol.30, No.1, pp. 15-25, 2006, DOI:10.32604/biocell.2006.30.015
Abstract A gene encoding a mannose-binding lectin, Pinellia pedatisecta agglutinin (PPA), was isolated from leaves of Pinellia pedatisecta using genomic walker technology. The ppa contained an 1140-bp 5’-upstream region, a 771-bp open reading frame (ORF) and an 829-bp 3’-downstream region. The ORF encoded a precursor polypeptide of 256 amino acid residues with a 24-amino acid signal peptide. There were one putative TATA box and six possible CAAT boxes lying in the 5’-upstream region of ppa. The ppa showed significant similarity at the nucleic acid level with genes encoding mannose-binding lectins from other Araceae species such as Pinellia ternata, Arisaema heterophyllum, Colocasia… More >
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