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    ARTICLE

    Stigma-Specific Comparative Proteomic Analysis Reveals the Distyly Response to Self-Incompatibility in Plumbago auriculata Lam

    Di Hu1, Shouli Yi1,*, Di Lin2, Suping Gao3, Ting Lei3, Wenji Li4, Tingdan Xu1, Songlin Jiang1

    Phyton-International Journal of Experimental Botany, Vol.93, No.4, pp. 681-697, 2024, DOI:10.32604/phyton.2024.049166

    Abstract In plants, heteromorphic self-incompatibility (HetSI) is a strategy for avoiding self-pollination and promoting outcrossing, and during this process, numerous protein-protein interaction events occur between the pistil and pollen. Previous studies in Primula and Fagopyrum that focused on HetSI systems have provided interesting insights; however, the molecular mechanism underlying HetSI remains largely unknown. In this study, we profiled the proteome of Plumbago auriculata stigmas before and after self-incompatible (SI) and self-compatible (SC) pollination. Comparative analyses were conducted by 4D-DIA (Four-dimensional data independent acquisition), a promising technology that increases the sensitivity and reduces the spectral complexity of proteomic analysis by adding a… More >

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