QIAOJIN JIA1,2, YUEJUN FU1,*
BIOCELL, Vol.47, No.3, pp. 627-645, 2023, DOI:10.32604/biocell.2023.026182
Abstract Background: The immune system of insects exerts fundamentally different antiviral mechanisms than
mammals. MicroRNAs (miRNAs) play vital roles in developing insect antiviral immunity. MiRNAs expression
profiles of insects changed significantly during baculovirus infection. Methods: Differential expression profiles of
miRNAs in Spodoptera frugiperda were monitored by next-generation sequencing (NGS) and RT-qPCR during
Autographa californica multiple nucleopolyhedrovirus (AcMNPV) infection. The transcription levels of genes were
detected by RT-qPCR. The 50% tissue culture infective dose (TCID50) endpoint dilution assay was used to determine
the proliferation of progeny virus. Results: NGS revealed that 49 miRNAs were differentially expressed in Sf9 cells,
and 10 of… More >
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