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  • Open Access

    ARTICLE

    Trilobatin Acts as a Marker Metabolite Involved in Flavonoid Accumulation Regulated by CsWRKY28-MYC2 with Trypsin Activation in Cucumber (Cucumis sativus)

    Enyan Chen1,#, Jingyu Jia1,3,#, Jiaju Sun1, Jie Wang2, Xinxin Chen1, Xin Li1,3,4,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.11, pp. 2837-2856, 2024, DOI:10.32604/phyton.2024.057932 - 30 November 2024

    Abstract During post-harvest storage of Cucumis sativus fruit, the application of trypsin treatment could increase flavonoid compound levels and reduce oxidative damage. To investigate the mechanism of trypsin-induced flavonoid biosynthesis in C. sativus, we conducted a combined analysis of transcriptomics and widely targeted metabolomics. One hundred and seventy-five significantly different metabolites were obtained from metabolomics data. The kyoto encyclopedia of genes and genomes (KEGG) functional enrichment results indicated that these metabolites were mainly involved in the phenylpropanoid biosynthesis pathway. By combining the results of the weighted gene co-expression network analysis (WGCNA) with the 130 upregulated phenylpropanoid metabolites, 22… More >

  • Open Access

    ARTICLE

    Uniting the Role of Entomopathogenic Fungi against Rhizoctonia solani JG Kühn, the Causal Agent of Cucumber Damping-Off and Root Rot Diseases

    Abdelhak Rhouma1, Lobna Hajji-Hedfi1,*, Nahla Alsayd Bouqellah2,*, Pravin Babasaheb Khaire3, Samar Dali1, Omaima Bargougui1, Amira Khlif1, Laith Khalil Tawfeeq Al-Ani4

    Phyton-International Journal of Experimental Botany, Vol.93, No.11, pp. 2857-2881, 2024, DOI:10.32604/phyton.2024.057591 - 30 November 2024

    Abstract Beauveria bassiana and Metarhizium spp. are entomopathogenic fungi with potential applications beyond insect pest control, including plant disease suppression, plant growth promotion, and rhizosphere colonization. This study investigated the plant growth-promoting characteristics and extracellular enzyme activities of Metarhizium spp. and B. bassiana in relation to phytopathogen interactions and plant growth. Additionally, the efficacy of these fungi in mitigating damping-off and root rot caused by Rhizoctonia solani on cucumber plants was evaluated in vitro and in vivo. Results indicate that B. bassiana and M. anisopliae produce indole-3-acetic acid, hydrocyanic acid, and hydrolytic enzymes. Seed treatment with these fungi significantly reduced disease severity (3.85%–1.86%, respectively)… More >

  • Open Access

    ARTICLE

    Contributions of Volume and Concentration on Runoff Nitrogen Losses from Intensive Vegetable in China

    Mingkun Cheng1,2, Ju Min2,*, Yanying Zhang2, Yuhe Wang3, Xia Wang4, Weiming Shi1,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.9, pp. 2439-2453, 2024, DOI:10.32604/phyton.2024.054182 - 30 September 2024

    Abstract Vegetable runoff nitrogen (N) loss is a serious environmental issue. However, whether the volume or N content of runoff determines the final N losses has not been clarified, which limited the optimal N managements in vegetable production. Here, we conducted a simulated rainfall experiment to study the runoff N loss flux pattern and the accumulation rate as well as the main influencing factors. The results showed that at 20 to 30 min, the volume of runoff water with a high N content reaches a critical inflection point of increase. Under 55 mm/h rainfall intensity, the… More >

  • Open Access

    ARTICLE

    Comparative Transcriptome Analyses Reveal Genes Related to Spine Development in Cucumber (Cucumis sativus)

    Yue Chen1,2,#, Huan Wang1,#, Taibai Xu1, Peng Zhou1, Junsong Pan2, Weiwei She1,*, Weiwei Zhang1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1059-1070, 2023, DOI:10.32604/phyton.2023.026486 - 06 January 2023

    Abstract Fruit spine is an important quality trait of cucumber. To better understand the molecular basis of cucumber spine development and function, RNA-Seq was performed to identify differentially expressed genes (DEGs) in fruit spines of different development stages, namely, 8 days before anthesis (SpBA8), anthesis (SpA) and 8 days after anthesis (SpAA8). Stage-wise comparisons obtained 2,259 (SpBA8 vs. SpA), 4,551 (SpA vs. SpAA8), and 5,290 (SpBA8 vs. SpAA8) DEGs. All the DEGs were classified into eight expression clusters by trend analysis. Among these DEGs, in addition to the Mict, Tril, CsTTG1, CsMYB6, NS, and Tu genes that have been reported to More >

  • Open Access

    ARTICLE

    Silicon and Nitric Oxide-Mediated Regulation of Growth Attributes, Metabolites and Antioxidant Defense System of Radish (Raphanus sativus L.) under Arsenic Stress

    Savita Bhardwaj1, Tunisha Verma1, Ali Raza2,*, Dhriti Kapoor1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 763-782, 2023, DOI:10.32604/phyton.2023.025672 - 29 November 2022

    Abstract

    Arsenic (As) contaminated food chains have emerged as a serious public concern for humans and animals and are known to affect the cultivation of edible crops throughout the world. Therefore, the present study was designed to investigate the individual as well as the combined effects of exogenous silicon (Si) and sodium nitroprusside (SNP), a nitric oxide (NO) donor, on plant growth, metabolites, and antioxidant defense systems of radish (Raphanus sativus L.) plants under three different concentrations of As stress, i.e., 0.3, 0.5, and 0.7 mM in a pot experiment. The results showed that As stress reduced the

    More >

  • Open Access

    ARTICLE

    Application of two forms of silicon and their impact on the postharvest and the content of bioactive compounds in cucumber (Cucumis sativus L.) fruits

    YOLANDA GONZÁLEZ-GARCÍA1, VALERIA FLORES-ROBLES2, GREGORIO CADENAS-PLIEGO3, ADALBERTO BENAVIDES-MENDOZA4, MARCELINO CABRERA DE LA FUENTE4, ALBERTO SANDOVAL-RANGEL4, ANTONIO JUÁREZ-MALDONADO1,*

    BIOCELL, Vol.46, No.11, pp. 2497-2506, 2022, DOI:10.32604/biocell.2022.021861 - 07 July 2022

    Abstract The metabolic activity of the fruits continues even after harvest, which results in the loss of bioactive compounds, a decrease in the quality of the fruits, softening and browning, among other negative effects. The use of certain elements such as silicon can improve postharvest quality, since it is involved in the metabolic, physiological and structural activity of plants, moreover can increase the quality of the fruits. In addition, nanotechnology has had a positive impact on crop yield, nutritional value, fruit quality and can improve antioxidant activity. For these reasons, the use of beneficial elements such… More >

  • Open Access

    ABSTRACT

    Image-Based Modeling for Atherosclerotic Coronary Plaque Progression and Vulnerability Research

    Rui Lv1, Liang Wang1, Dalin Tang1,*,2

    Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 27-28, 2019, DOI:10.32604/mcb.2019.06829

    Abstract Medical imaging and image-based computational modeling have been used by many researchers in recent years to quantify atherosclerotic plaque morphological and biomechanical characteristics and predict the coronary plaque growth and rupture processes. However, it has been hard to validate model predictions due to imaging resolution limitation, lack of clinical events and plaque rupture data. This article reviews recent advances in coronary plaque research over the past decade, including medical imaging techniques represented by intravascular ultrasound (IVUS) and optical coherence tomography (OCT), computational modeling and their applications in plaque progression and vulnerability analyses and predictions. The… More >

  • Open Access

    ABSTRACT

    Predicting Plaque Progression Using Patient-Specific Fluid-Structure-Interaction Models Based on IVUS and OCT Images with Follow-Up

    Xiaoya Guo1, Dalin Tang1,2,*, David Molony3, Chun Yang2, Habib Samady3, Jie Zheng4, Gary S. Mintz5, Akiko Maehara5, Jian Zhu6, Genshan Ma6, Mitsuaki Matsumura5, Don P. Giddens3,7

    Molecular & Cellular Biomechanics, Vol.16, Suppl.1, pp. 75-76, 2019, DOI:10.32604/mcb.2019.05743

    Abstract Atherosclerotic plaque progression is generally considered to be closely associated with morphological and mechanical factors. Plaque morphological information on intravascular ultrasound (IVUS) and optical coherence tomography (OCT) images could complement each other and provide for more accurate plaque morphology. Fluid-structure interaction (FSI) models combining IVUS and OCT were constructed to obtain accurate plaque stress/strain and flow shear stress data for analysis. Accuracy and completeness of imaging and advanced modeling lead to accurate plaque progression predictions.
    In vivo IVUS and OCT coronary plaque data at baseline and follow-up were acquired from left circumflex coronary and right coronary… More >

  • Open Access

    ARTICLE

    In vitro plant regeneration via indirect organogenesis from different explants of Lathyrus sativus L. and Lathyrus cicera L.

    Li RS1, YJ Tao2, FJ Liu2, X Hu2, QL Xu2, KY Li2

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 87-93, 2016, DOI:10.32604/phyton.2016.85.087

    Abstract The grass pea (Lathyrus sativus L.) and flatpod peavine (Lathyrus cicera L.) are the most economically important and widely cultivated Lathyrus species. However, their utilization is limited due to the presence of their endogenous toxin β-N-oxalyl-L-α, β-diaminopropionic acid (β-ODAP). Thus, a β-ODAP free variety should be developed through some plant breeding technique like either mutational breeding or genetic-manipulation. In this circumstance, the plant regeneration of Lathyrus species becomes a bottleneck. In the present study, an efficient system for in vitro regeneration of L. sativus with high β-ODAP levels, and L. cicera with low β-ODAP levels, was developed from different explants (axillary… More >

  • Open Access

    ARTICLE

    Using 2D In Vivo IVUS-Based Models for Human Coronary Plaque Progression Analysis and Comparison with 3D Fluid-Structure Interaction Models: A Multi-Patient Study

    Hongjian Wang*, Jie Zheng, LiangWang, Akiko Maehara§, Chun YangII, David Muccigrosso, Richard BachkII, Jian Zhu**, Gary S. Mintz§, Dalin Tang*,‡,††

    Molecular & Cellular Biomechanics, Vol.12, No.2, pp. 107-122, 2015, DOI:10.3970/mcb.2015.012.107

    Abstract Computational modeling has been used extensively in cardiovascular and biological research, providing valuable information. However, 3D vulnerable plaque model construction with complex geometrical features and multicomponents is often very time consuming and not practical for clinical implementation. This paper investigated if 2D atherosclerotic plaque models could be used to replace 3D models to perform correlation analysis and achieve similar results. In vivo intravascular ultrasound (IVUS) coronary plaque data were acquired from a patient follow-up study to construct 2D structure-only and 3D FSI models to obtain plaque wall stress (PWS) and strain (PWSn) data. One hundred… More >

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