SHADY G. EL-SAWAH^1,*, FAYEZ ALTHOBAITI², ADIL ALDHAHRANI³, EMAN FAYAD², MARWA A. ABDEL-DAYEM⁴, REHAB M. AMEN⁵, EL SHAIMAA SHABANA⁶, EHAB I. EL-HALLOUS⁷, HANAN M. RASHWAN¹

BIOCELL, Vol.45, No.6, pp. 1561-1568, 2021, DOI:10.32604/biocell.2021.016869

Abstract Diabetes mellitus (DM) could negatively affect patients’ health via inducing a lot of serious functional hazards in many tissues’ cells at molecular levels. Recently, many scientists had proposed stem cell therapy being an appropriate alternative treatment protocol for numerous health threatening issues including diabetes. Therefore, the current study was designed to investigate the antioxidant potentiality of two MSCs types in alleviating tissues’ oxidative stress dramatic elevation resulting as a consequence of Type 1 DM induction. In our 4 weeks study, animals were divided into four groups: control group, STZ-diabetic group (D), D+AD-MSCs group and D+BM-MSCs group. Data reported that diabetic… More >

Ibrahim M. Almanjahie^1,2, Amer Ibrahim Al-Omari^3,*, Emmanuel J. Ekpenyong⁴, Mir Subzar⁵

Computer Systems Science and Engineering, Vol.38, No.1, pp. 1-15, 2021, DOI:10.32604/csse.2021.015933

Abstract In estimation theory, the researchers have put their efforts to develop some estimators of population mean which may give more precise results when adopting ordinary least squares (OLS) method or robust regression techniques for estimating regression coefficients. But when the correlation is negative and the outliers are presented, the results can be distorted and the OLS-type estimators may give misleading estimates or highly biased estimates. Hence, this paper mainly focuses on such issues through the use of non-conventional measures of dispersion and a robust estimation method. Precisely, we have proposed generalized estimators by using the ancillary information of non-conventional measures… More >

SHUCAI WU^1,#, DENGRUI LI^1,#, SUMIN GUO¹, LI GAO², YONGHUI YANG^1,*

BIOCELL, Vol.45, No.2, pp. 339-344, 2021, DOI:10.32604/biocell.2021.011379

Abstract Since stem cell therapy is the most effective treatment in the field of tissue reparation and reconstitution, the present study aimed to explore the different sources of mesenchymal stem cells (MSCs) on the different effects of pulmonary fibrosis-related cytokines in C57BL/6 mice. For reaching this goal, we isolated MSCs from umbilical cord blood and placenta and used for stem cell therapy in a mouse model of pulmonary fibrosis model. The pulmonary fibrosis model was done by injecting bleomycin into the trachea of C57BL/6 mice. Then we assessed the degree of pulmonary fibrosis in each mouse lung tissue at weeks 1,… More >

Osama M. AHMED¹, Mohamed A. HASSAN², Ablaa S. SALEH²

BIOCELL, Vol.44, No.1, pp. 27-29, 2020, DOI:10.32604/biocell.2020.08040

Abstract This study aimed to assess the effect of hesperetin and/or bone marrow-derived mesenchymal stem cells (BM-MSCs) on disturbed lipid profile, heart and kidney functions, oxidative stress and antioxidant defense system in streptozotocin (STZ)-induced diabetic rats. Type 1 diabetes mellitus (T1DM) was induced in male Wistar rats by injecting 40 mg/kg body weight (b.w.) STZ dissolved in citrate buffer (pH 4.5). The diabetic rats were treated with hesperetin orally administered at dose 20 mg/kg b.w., BM-MSCs intravenously injected at a dose of 1 x 106 cells/ rat/week and their combination for 6 weeks. The diabetic rats exhibited lipid abnormalities manifested by… More >

Lili Dong¹, Yang Song^1,*, Li Yang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 78-78, 2019, DOI:10.32604/mcb.2019.07130

Abstract It has been widely recognized that stem cells possess the potential of osteogenic differentiation, which greatly contribute to bone repair. Recently, accumulating evidences have indicated that mechanical cues are required for bone repair ^[1,2]. However, how local and recruited stem cells in the bone architecture receive the mechanical signals is poorly understood ^[3,4]. The purpose of this study is to demonstrate that macrophages potentially transduce the mechanical signals for stem cell osteogenic lineage. This demonstration has been carried out through a co-culture system to investigate the effect of macrophages which subjected to cyclic stretch on the osteogenic potential of bone… More >

Pranav S. Sapkal^1*, Abhaykumar M. Kuthe¹, Divya Ganapathy², Shantanu C. Mathankar³, Sudhanshu Kuthe⁴

Molecular & Cellular Biomechanics, Vol.13, No.2, pp. 115-136, 2016, DOI:10.3970/mcb.2016.013.131

Abstract Biphasic calcium phosphate scaffolds with 20/80 HA/TCP ratio were fabricated using the 3D-Bioplotting system to heal critical size defects in rabbit tibia bone. Four different architectures were printed in a layer by layer fashion with lay down patterns viz. (a) 0°– 90°, (b) 0°– 45°– 90°– 135°, (c) 0°–108°– 216° and (d) 0°– 60°– 120°. After high-temperature sintering scaffolds were coated with collagen and were further characterized by (FTIR) Fourier Transform Infrared Spectroscopy, (SEM) Scanning Electron Microscopy, (XRD) X-Ray diffraction, Porosity analysis and Mechanical testing. Scaffold samples were tested for its ability to induce cytotoxicity in Balb/c 3T3 cells at… More >

Syed A Jamal^*

Molecular & Cellular Biomechanics, Vol.10, No.4, pp. 267-273, 2013, DOI:10.3970/mcb.2013.010.267

Abstract Human Umbilical Cord Mesenchymal Stem Cells (hUCMSCs) were subjected to in vitro osteogenic differentiation using a novel combination of signaling molecules including BMP-2 and purmorphamine. Differentiation outcomes were assessed by calcein staining and by microscopic examination of the cytoskeleton. Calcein staining showed appreciable degree of calcium mineralization in cell culture, and changes in the morphological attributes of differentiating cells were observed vis-a-vis the actin cytoskeleton. Finally, positive calcein staining, altered cytoskeletal profile, and stress fiber formation in treated cells demonstrated, for the first time, a potentially synergistic interplay between BMP-2 and the hedgehog agonist, purmorphamine. This study lends support to… More >

Masanori Kobayashi, Myron Spector

Molecular & Cellular Biomechanics, Vol.6, No.4, pp. 217-228, 2009, DOI:10.3970/mcb.2009.006.217

Abstract In order to achieve successful wound repair by regenerative tissue engineering using mesenchymal stem cells (MSCs), it is important to understand the response of stem cells in the scaffold matrix to mechanical stress.
To investigate the clinical effects of mechanical stress on the behavior of cells in scaffolds, bone marrow-derived mesenchymal stem cells (MSCs) were grown on a type-I collagen-glycosaminoglycan (GAG) scaffold matrix for one week under cyclic stretching loading conditions.
The porous collagen-GAG scaffold matrix for skin wound repair was prepared, the harvested canine MSCs were seeded on the scaffold, and cultured under three kinds of cyclic… More >

Donald F. Ward Jr.^*, William A. Williams^*, Nicole E. Schapiro^*, Samuel R. Christy^*, Genevieve L. Weber^*, Megan Salt, Robert F. Klees^*, Adele Boskey^†, George E. Plopper ^∗,‡

Molecular & Cellular Biomechanics, Vol.4, No.4, pp. 177-188, 2007, DOI:10.3970/mcb.2007.004.177

Abstract Focal adhesion kinase (FAK) is a key integrator of integrin-mediated signals from the extracellular matrix to the cytoskeleton and downstream signaling molecules. FAK is activated by phosphorylation at specific tyrosine residues, which then stimulate downstream signaling including the ERK1/2 pathway, leading to a variety of cellular responses. In this study, we examined the effects of FAK point mutations at tyrosine residues (Y397, Y925, Y861, and Y576/7) on osteogenic differentiation of human mesenchymal stem cells exposed to collagen I and cyclic tensile strain. Our results demonstrate that FAK signaling emanating from Y397, Y925, and to a lesser extent Y576/7, but not… More >

M. Dumbser¹, D.S. Balsara²

CMES-Computer Modeling in Engineering & Sciences, Vol.54, No.3, pp. 301-334, 2009, DOI:10.3970/cmes.2009.054.301

Abstract In this article we use the new, unified framework of high order one-step P_NP_M schemes recently proposed for inviscid hyperbolic conservation laws by Dumbser, Balsara, Toro, and Munz (2008) in order to solve the viscous and resistive magnetohydrodynamics (MHD) equations in two and three space dimensions on unstructured triangular and tetrahedral meshes. The P_NP_M framework uses piecewise polynomials of degree N to represent data in each cell and piecewise polynomials of degree M ≥ N to compute the fluxes and source terms. This new general machinery contains usual high order finite volume schemes (N = 0) and discontinuous Galerkin finite… More >