FENGLIN BAI1,2,#, BIANXIA BAI1,2,#, TINGTING JIN1,2, GUIPING ZHANG1,2, JIAHONG REN1,2,*
BIOCELL, Vol.47, No.3, pp. 647-656, 2023, DOI:10.32604/biocell.2023.024758
Abstract Background: Serratia ureilytica DW2 is a highly efficient phosphate-solubilizing bacteria isolated from
Codonopsis pilosula rhizosphere soil that can promote the growth of C. pilosula; nonetheless, until now, no validated
reference genes from the genus Serratia have been reported that can be used for the normalization of quantitative
real-time polymerase chain reaction (RT–qPCR) data. Methods: To screen stable reference genes of S. ureilytica
DW2, the expression of its eight candidate reference genes (16S rRNA, ftsZ, ftsA, mreB, recA, slyD, thiC, and zipA)
under different treatment conditions (pH, temperature, culture time, and salt content) was assayed by RT–qPCR. The
expression stability of… More >
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