MENGMENG WU^1,#, PENG GUO^1,#, YISU SHI^1,#, DANYAN ZHENG¹, QIAONAN ZHANG¹, XIN JIN¹, YUHUA WANG¹, QIANG LIN², RONGJUN FANG^1,*, WEIGUO ZHAO^1,*

BIOCELL, Vol.46, No.8, pp. 1935-1945, 2022, DOI:10.32604/biocell.2022.019223

Abstract A variety of plants were colchicine treated to double their chromosome number. Chromosomes are genetic carriers that determine the individual traits of organisms. The doubling of chromosomes will lead to modifications in plant morphology, physiology and genetics. To determine the response of mulberry trees induced by colchicine, using mulberry variety Yu-711 leaves as research materials, two small RNA libraries (control and experimental groups) were constructed. It was found that 45 known miRNA genes and 78 predicted novel miRNA genes in the sequence results. A comparison of data between the control group and the experimental group revealed 37 differentially expressed miRNA… More >

PRIYANKA THAKUR¹, REENA V. SAINI^2,3,*, ANIL K. CHHILLAR⁴, NEERAJ K. SAINI⁵, VIJAY KUMAR THAKUR⁶, SAMARJEET SINGH SIWAL⁷, ADESH K. SAINI^2,3,*

BIOCELL, Vol.46, No.2, pp. 309-324, 2022, DOI:10.32604/biocell.2022.016916

Abstract Breast cancer, also recognized as the principal cause of cancer-related deaths among women, is the second most familiar and prevalent form of cancer. New diagnostic and prognostic biomarkers that are highly specific are urgently needed for its early prognosis. MicroRNAs (miRNAs), a class of non-coding RNAs, are known to control the biological processes involving transcription, post-transcriptional and covalent modifications, splicing, translation, cell differentiation, proliferation, apoptosis, cancer progression, and invasion. Any dysregulation in miRNA expression, demonstrating their oncogenic and tumor-suppressive functions, contributes to cancer progression. MicroRNA-21 (miR-21), an ‘onco-miR’ in breast cancer, is involved in tumor progression and metastasis by suppressing… More >

Yongxia He¹, Zheng Wang¹, Yue Wang², Man Sun^3,*

Oncologie, Vol.23, No.3, pp. 351-357, 2021, DOI:10.32604/Oncologie.2021.014772

Abstract Gastric cancer is a highly malignant disease with complex pathogenic mechanisms, and has high incidence and mortality rate. At present, the diagnosis of gastric cancer mainly includes gastroscopy, serum analysis and needle biopsy, and the treatment methods include conventional surgical resection, radiotherapy and chemotherapy. Yet, some limitations were involved in these diagnostic and therapeutic methods, so accurate targeted therapy has received considerable attention. MicroRNAs (miRNAs) are non-coding RNA that can interact with the 3-terminal non-translational region of the target gene mRNA to reduce the expression of the target gene, participate in the regulation of multiple signaling pathways, and play an… More >

Dan Wang¹, Fengjuan Lu¹, Pengkai Wang¹, Jiaji Zhang¹, Lu Lu¹, Yan Zhen¹, Ye Lu¹, Tielong Cheng², Jisen Shi^1,*, Jinhui Chen^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.5, pp. 1445-1463, 2021, DOI:10.32604/phyton.2021.015499

Abstract Somatic cells respond to considerable stress, and go through a series of phytohormone pathways, then forming an embryo. The developmental process is recorded as somatic embryogenesis (SE). One of the key components regulating SE are the microRNAs (miRNAs). Despite previous studies, it is still not clear exactly how miRNAs exert their function of regulating targets during conditionally activated early SE. Here, we use Liriodendron sino-americanum as a model system and perform a combined analysis of microfluidic chips and degradome sequencing to study this process. We identified a total of 386 conserved miRNAs and 153 novel miRNAs during early SE. According… More >

Chongchong Yan¹, Qianqian Wang¹, Nan Zhang², Jiajia Wang¹, Xuexiang Ren¹, Bingjie Xue³, Xiaojing Pu⁴, Zhihong Xu⁴, Huajun Liao^1,*

Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 561-586, 2020, DOI:10.32604/phyton.2020.010322

Abstract Since potato cultivars are sensitive to low temperature, cold injury severely affects the geographical distribution and yield of potato. Although some miRNAs have been identified in response to cold stress in plants, there is no report about the role of miRNAs in the response to cold stress in potato. Here, via high throughput sequencing, we described the profiling of cold stress response to miRNA and mRNA in potato. Two small RNA and six mRNA libraries were constructed and sequenced. 296 known and 211 novel miRNAs were identified, in which 34 miRNAs in Cold Group (CG) had the higher expression quantity… More >