Ojeda-Zacarías1 Ma del Carmen, Hugo A Luna-Olvera2, Lilia H Morales-Ramos2, María J Verde-Star2, Teresa E Torres-Cepeda2, Benito Pereyra-Alférez2, Leobardo Iracheta-Donjuan3, Emilio Olivares-Sáenz4, Raúl Salazar-Sáenz4, Elizabeth Cárdenas-Cerda4
Phyton-International Journal of Experimental Botany, Vol.75, pp. 109-113, 2006, DOI:10.32604/phyton.2006.75.109
Abstract Pinus maximartinezii (Rzedowski) is an endemic endangered species, confined to a single population of approximately 2000 to 2500 mature trees. It covers about 400 ha in southern Zacatecas, Mexico. The success of tissue culture techniques for germplasm preservation depends on regeneration of cultures. The objective of this study was to achieve an in vitro proliferation protocol using organogenesis technique for Pinus maximartinezii. Mature seeds were surface sterilized in 6% H2O2 v/v. Isolated cotyledons and zygotic embryos were cultured on shoot induction media. DCR and GD media were supplemented with 0.3 and 0.5 mgl-1 BAP; 0.01 mgl-1 ANA and vitamin solution.… More >
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