JINGYAO XU^1,#, SHUANGLI HAO^1,#, KAIYUE HAN^1,#, WANXI YANG^1,*, HONG DENG^2,*

BIOCELL, Vol.47, No.4, pp. 773-788, 2023, DOI:10.32604/biocell.2023.026618

Abstract As a pathway that plays a role in nutrient absorption, anabolic response, cell growth and survival, the important role of AKT/mTOR in tumorigenesis has also come to light. For cancer patients, most deaths are caused by the growth of metastatic tumors outside the primary focus. Therefore, migration and invasion in the late stage of tumor progression are the main unresolved issues in the study of tumor pathogenesis, and AKT/mTOR has been found to participate in the migration and invasion of cancer cells, which means that the study of this pathway may contribute to a solution for the problem. Because of… More >

ZHEN JIA^1,2,#, ZHENGTING QIAN^1,2,#, YONG TANG², XIANG LI², YAN SHI², HENG XIN^1,2, YOUWU FAN^1,2,*, HEMING WU^2,*

Oncology Research, Vol.29, No.2, pp. 105-117, 2021, DOI:10.32604/or.2022.03536

Abstract Glioma is a general malignant tumor with a dismal prognosis. Long noncoding RNAs (lncRNAs) have been implicated in the initiation and processes of tumors. An investigation of the GEPIA database revealed that long noncoding RNA WEE2 antisense RNA 1 (WEE2-AS1) is upregulated in glioma tissues compared to normal brain tissues, and validation with quantitative real-time polymerase chain reaction (qRT–PCR) revealed that WEE2-AS1 expression was consistent with the database prediction. Fluorescence in situ hybridization (FISH) assays revealed that WEE2-AS1 was localized primarily in the cytoplasm. Clone formation experiment and EDU assay were used to detect cell proliferation ability, and Transwell assay… More >

Genglong Zhu^*, Xialei Liu^*, Yonghui Su^†, Fangen Kong^‡, Xiaopeng Hong^*, Zhidong Lin^†

Oncology Research, Vol.27, No.1, pp. 55-64, 2019, DOI:10.3727/096504018X15201143705855

Abstract Liver cancer is one of the most common malignancies in the world and a leading cause of cancer-related mortality. Accumulating evidence has highlighted the critical role of long noncoding RNAs (lncRNAs) in various cancers. The present study aimed to explore the role of lncRNA urothelial carcinoma-associated 1 (UCA1) in cell growth and migration in MHCC97 cells and its underlying mechanism. First, we assessed the expression of UCA1 in MHCC97 and three other cell lines by RT-qPCR. Then the expression of UCA1, miR-301a, and CXCR4 in MHCC97 cells was altered by transient transfection. The effects of UCA1 and miR-301 on cell… More >

Ni Kou^*1, Sha Liu^*1, Xiaojie Li^*, Wuwei Li^*, Weijian Zhong^*, Lin Gui^*, Songling Chai^*, Xiang Ren^*, Risu Na^*, Tao Zeng^†, Huiying Liu^*

Oncology Research, Vol.27, No.2, pp. 173-182, 2019, DOI:10.3727/096504018X15202945197589

Abstract The long noncoding RNA (lncRNA) H19 has been described to participate in the metastasis of various tumors. Nevertheless, whether H19 promotes or impedes tongue squamous cell carcinoma (TSCC) cell migration and invasion remains controversial. Here we found that the expression of H19 was elevated in TSCC tissues compared with adjacent normal tissues. Moreover, we demonstrated that the expression of H19 was higher in metastasized tumors compared with unmetastasized tumors. Consistently, TSCC cells express higher levels of H19 than human squamous cells. Subsequently, depletion of H19 impaired the migration and invasion abilities of TSCC cells. Mechanistically, we demonstrated that H19 functions… More >

Jian-Wei Wang, Xiao-Feng Wu, Xiao-Juan Gu, Xing-Hua Jiang

Oncology Research, Vol.27, No.9, pp. 979-986, 2019, DOI:10.3727/096504018X15336368805108

Abstract Cancer-associated fibroblasts (CAFs) play a predominant role in regulating tumor progression. Understanding how CAFs communicate with osteosarcoma is crucial for developing novel approaches for osteosarcoma therapy. Exosomes are able to transmit messages between cells. In this study, we demonstrated that CAFs transfer exosomes to osteosarcoma cells, which promotes osteosarcoma cell migration and invasion. Using a miRNA microarray analysis, we identified 13 miRNAs that are significantly increased in exosomes derived from cancer-associated fibroblasts (CAFs) and corresponding paracancer fibroblasts (PAFs). In vitro studies further validated that the levels of microRNA-1228 (miR-1228) were increased in CAFs, its secreted exosomes, and in recipient osteosarcoma… More >

Yankun Zhang^*, Wei Qian^*, Feng Feng^†, Qian Cao^*, Yanqi Li^*, Ying Hou^*, Luyang Zhang^*, Jufeng Fan^*

Oncology Research, Vol.27, No.3, pp. 371-377, 2019, DOI:10.3727/096504018X15178740729367

Abstract This study aimed to investigate the effect and underlying mechanism of lncRNA CASC2 in malignant melanoma (MM). Expression of CASC2 in MM tissues and cells was detected. A375 cells were transfected with pc-CASC2, si-CASC2, miR-18a-5p inhibitor, or corresponding controls, and then cell proliferation, migration, and invasion were detected using MTT assay, colony formation assay, and Transwell analysis, respectively. The relationship of miR-18a-5p and CASC2 or RUNX1 was detected by luciferase reporter assay. The levels of CASC2 and RUNX1 were significantly reduced in MM tissues compared with normal skin tissues or cells, while the miR-18a-5p level was obviously increased (all p<0.01).… More >

WM Farhan Syafiq B. WM Nor^*†, Ivy Chung^‡§, Nur Akmarina B. M. Said^†

Oncology Research, Vol.28, No.6, pp. 615-629, 2020, DOI:10.3727/096504020X16037933185170

Abstract Breast cancer is the most commonly diagnosed cancer among women and one of the leading causes of cancer mortality worldwide, in which the most severe form happens when it metastasizes to other regions of the body. Metastasis is responsible for most treatment failures in advanced breast cancer. Epithelial–mesenchymal transition (EMT) plays a significant role in promoting metastatic processes in breast cancer. MicroRNAs (miRNAs) are highly conserved endogenous short noncoding RNAs that play a role in regulating a broad range of biological processes, including cancer initiation and development, by functioning as tumor promoters or tumor suppressors. Expression of miR-548m has been… More >

RUONAN GU^1,2, WENJING GUO², WENYANG WANG¹, GUIHUAN LI¹, XIAOJU LAI¹, ZHIBIN HUANG¹, WANLU ZHAO¹, ZHICONG WU¹, HUA CHEN¹, WENYANG LUO¹, FANGYIN ZENG^3,*, FAN DENG^1,*

BIOCELL, Vol.45, No.6, pp. 1585-1599, 2021, DOI:10.32604/biocell.2021.017016

Abstract Propofol, a common intravenous anesthetic used in clinical, has been shown to regulate cells proliferation, inflammation, angiogenesis and metastasis as well as exerting anti-cancer effect in several different types of cancer. However, the functions and mechanisms of propofol in lactate secretion and invasion of cancer cells remain unknown. The primary aim of this study was to investigate the role of propofol in cells migration and invasion of colon cancer (CRC). Scratches assay, Transwell assay were used to detect colon cells migration and invasion. Real-time Quantitative Polymerase Chain Reaction (RT-qPCR), Western blotting were utilized to detect the expression of related molecules.… More >

BENJIANG QIAN¹, XIAOYAN YING², GUANG YANG¹, HUIZHANG LI³, JIANMING TAN^1,*

BIOCELL, Vol.45, No.3, pp. 577-588, 2021, DOI:10.32604/biocell.2021.014972

Abstract This work aimed to discover new therapeutic targets in renal clear cell carcinoma by bioinformatics and detect the effect of candidate gene TRIP13 in renal cell carcinoma (RCC) cell proliferation, migration, and invasion. Differentially expressed mRNAs were screened based on The Cancer Genome Atlas (TCGA)-Kidney Renal Clear Cell Carcinoma (KIRC) databases, and functional enrichments, survival analysis, receiver operating characteristic curve (ROC), and Protein–Protein Interaction (PPI) protein interaction analysis were performed by R software to screen the candidate gene TRIP13. Then, the expression of candidate gene TRIP13 in 92 pairs of cancer and adjacent normal tissues of renal clear cell carcinoma… More >