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Knockdown of Long Noncoding RNA uc.338 by siRNA Inhibits Cellular Migration and Invasion in Human Lung Cancer Cells

Xuexin Gao*, Xuezhen Gao, Chao Li*, Yukun Zhang*, Lei Gao

* Department of Thoracic Surgery, Central Hospital of Tai’an, Tai’an, Shandong, China
† Operation Room, People’s Hospital of Xin Tai, Xin Tai, Shandong, China
‡ Shandong Provincial Research Center for Bioinformatic Engineering and Technique, School of Life Sciences, Shandong University of Technology, Zibo, Shandong, China

Oncology Research 2016, 24(5), 337-343. https://doi.org/10.3727/096504016X14666990347671

Abstract

Lung cancer remains a critical health concern worldwide. Long noncoding RNAs with ultraconserved elements have recently been implicated in human tumorigenesis. The present study investigated the role of ultraconserved element 338 (uc.338) in the regulation of cell proliferation and metastasis in human lung cancer. Our data showed that the expression of uc.338 in lung cancer was remarkably increased in vivo and in vitro. Depletion of uc.338 with specific siRNA interference retarded the cell proliferative rate in lung cancer cell lines NCI-H929 and H1688. Furthermore, knockdown of uc.338 caused cell cycle arrest in the G0/G1 phase in both cell lines. Transwell assays showed that inhibition of uc.338 notably decreased migration and invasion in NCI-H929 and H1688 cells. Moreover, uc.338 depletion decreased the expression of cyclin B1, Cdc25C, Snail, vimentin, and N-cadherin while increasing the protein level of E-cadherin, shown with Western blot analysis. These results suggested the pro-oncogenic potential of uc.338 in lung cancer, which might provide novel clues for the diagnosis and treatment of lung cancer in the clinic.

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APA Style
Gao, X., Gao, X., Li, C., Zhang, Y., Gao, L. (2016). Knockdown of long noncoding RNA uc.338 by sirna inhibits cellular migration and invasion in human lung cancer cells. Oncology Research, 24(5), 337-343. https://doi.org/10.3727/096504016X14666990347671
Vancouver Style
Gao X, Gao X, Li C, Zhang Y, Gao L. Knockdown of long noncoding RNA uc.338 by sirna inhibits cellular migration and invasion in human lung cancer cells. Oncol Res. 2016;24(5):337-343 https://doi.org/10.3727/096504016X14666990347671
IEEE Style
X. Gao, X. Gao, C. Li, Y. Zhang, and L. Gao "Knockdown of Long Noncoding RNA uc.338 by siRNA Inhibits Cellular Migration and Invasion in Human Lung Cancer Cells," Oncol. Res., vol. 24, no. 5, pp. 337-343. 2016. https://doi.org/10.3727/096504016X14666990347671



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