Ruíz-Salazar R¹, JS Muruaga-Martínez², MLP Vargas-Vázquez², G Alejandre-Iturbide⁴, G Castañón-Nájera³, S Hernández-Delgado¹, N Almaraz-Abarca⁴, N Mayek-Pérez^1,5

Phyton-International Journal of Experimental Botany, Vol.85, pp. 184-193, 2016, DOI:10.32604/phyton.2016.85.184

Abstract In order to identify genes of resistance to four diseases that affect Phaseolus sp. [i.e, Common Bacterial Blight (CBB), Bean Common Mosaic Virus (BCMV), Bean Golden Mosaic Virus (BGMV) and angular leaf spot (ALS)], we amplified nine SCAR (Sequence Characterized Amplified Regions) markers: four linked to resistance to common blight, two for BCMV, two for BGMV and one for ALS. The genetic material included 16 ayocote bean (P. coccineus) populations collected from different locations at Veracruz and Puebla, Mexico. Populations from Veracruz showed greater number of SCARs for BGMV and ALS compared with populations from Puebla. The best P. coccineus… More >

Hernández-Ibáñez L, J Sahagún-Castellanos, JE Rodríguez-Pérez, MG Peña-Ortega, LM Rodríguez-Martínez

Phyton-International Journal of Experimental Botany, Vol.83, pp. 311-318, 2014, DOI:10.32604/phyton.2014.83.311

Abstract In many countries, tomato (Solanum lycopersicum L.) is the most important horticultural species, but good variety seeds are expensive. In hybrid breeding, the number of possible single crosses is too large with only a few lines. With 60 lines, for example, 1770 single-cross hybrids can be formed. This makes it expensive and even impossible to conduct an adequate experimental evaluation. These cases require the availability of methods for predicting hybrid performance. This study was designed to evaluate a method to predict fruit yield in tomato hybrids based on genomic fingerprints, the theory of mixed models, and the experimental evaluation of… More >

Martínez ME¹, JM Lesher G², G Castañón N², E de la Cruz L¹, C Zapata H²

Phyton-International Journal of Experimental Botany, Vol.82, pp. 209-214, 2013, DOI:10.32604/phyton.2013.82.209

Abstract Nanche (Byrsonima crassifolia (L.) H.B.K.) grows in the southeastern region of the Mexican republic under both wild and semi-cultivated forms. Despite the importance of the fruit of this tree, only one paper at the molecular level has been published in Mexico, and possibly in America, to date. For this reason, the purpose of this research was to study the genetic variability of nanche trees that grow under both wild and semi-cultivated conditions in the state of Tabasco, Mexico. Molecular determinations were carried out in the Laboratorio de Genómica of the División Académica de Ciencias Biológicas (DACBiológicas), Universidad Juárez Autónoma de… More >

Escalante-González JG¹, EA García-Zambrano², A Gutiérrez-Diez², RE Vázquez-Alvarado², JA Torres-Castillo², SR Sinagawa-García², F Zavala-García², J Martínez-de la Cerda², C Gallegos-Vázquez³, JI García-Zambrano⁴

Phyton-International Journal of Experimental Botany, Vol.81, pp. 143-148, 2012, DOI:10.32604/phyton.2012.81.143

Abstract The objective of this research was to increase the genetic diversity of the Nopal Germplasm Bank of the FAUANL using RAPD molecular markers to estimate the genetic diversity of 15 accessions of cactus pear from molecular markers recorded in the Bank. DNA extraction was performed by the method of cellular ruptor using FastDNA^® Kit. Performance measurement of DNA was carried out through Picoflour. It was observed that the DNA concentrations ranged from 74.91 ng/µL to 12.32 ng/uL. Twelve primers were used to generate from 1 to 5 bands per primer with a molecular weight ranging from 200 to 1000 bp.… More >

Zappacosta D¹, M Meier¹, A Carrera¹, G Pacheco², S Cardone³, JP Selva¹, V Echenique¹

Phyton-International Journal of Experimental Botany, Vol.80, pp. 211-220, 2011, DOI:10.32604/phyton.2011.80.211

Abstract Weeping lovegrass is well adapted for forage production and useful for soil conservation in semiarid regions, constituting a morphologically diverse group. Diploid genotypes are unfrequent and reproduce sexually, whereas the tetraploids and plants of higher ploidy levels reproduce by apomixis. In the present work RAPD, AFLP and EST-SSR were used in order to assess the reproductive mode through progeny tests, to determine intracultivar homogeneity or seed purity, to establish genetic relationships among the cultivars within the complex and to characterize the new materials obtained by our group. Eight commercial cultivars and three new plant materials were analyzed. Uniform and variable… More >

Alejandre Rosas¹ JA, ME Galindo Tovar², HE Lee Espinosa², OG Alvarado Gómez³

Phyton-International Journal of Experimental Botany, Vol.79, pp. 87-94, 2010, DOI:10.32604/phyton.2010.79.087

Abstract Development of new sugarcane varieties allows to increase sucrose production and faces challenges as new pests and diseases, and soil fertility losses. The objective of this study was to contribute to expand molecular tools in selecting useful progenitors for hybridization programs. With this purpose, 14 RAPDs primers were pre-screened, and four of them which produced clearly resolved, reproducible and polymorphic fragment patterns were selected for genetic analysis of 22 sugarcane variety hybrids. These were used in the Estación de Hibridación at Tapachula, Chiapas, as progenitors in genetic improvement programs. Genetic distance analysis showed three main clusters. The first cluster contained… More >

García-Zambrano² EA, F Zavala-García², A Gutiérrez-Diez, MC Ojeda-Zacarías, I Cerda-Hurtado³

Phyton-International Journal of Experimental Botany, Vol.78, pp. 117-120, 2009, DOI:10.32604/phyton.2009.78.117

Abstract The objective of this study was to apply the AFLP-type molecular marker technique to estimate genetic diversity in cactus pear within the Germplasm Bank of FAUANL. Twelve accessions have been reported as possible duplicates through type RAPD- molecular markers in such Bank. DNA was extracted using the cellular ruptor technique, and then digested and bound. A preamplification and subsequent selective amplification were conducted. Amplified fragments were then separated and analyzed. It was concluded that none of the accessions was duplicated. This was because 8 groups were formed in the dendogram obtained after applying the UPGMA method. There were 3 accessions… More >

Picca¹ A, P Roncallo¹, A Carrera¹, G Cervigni¹, R. Miranda², V Echenique¹

Phyton-International Journal of Experimental Botany, Vol.77, pp. 175-188, 2008, DOI:10.32604/phyton.2008.77.175

Abstract The aims of this work were (1) the saturation of a linkage map of durum wheat using AFPLs and RAPDs, (2) mapping of QTL related to lipoxygenase activity and (3) estimation of its usefulness in marker-assisted selection to increase pasta colour. A mapping population of 83 recombinant lines (RILs) derived from the cross between durum wheat Kofa and UC1113 (Triticum turgidum L. var. durum) was evaluated for lipoxygenase activity during two growing seasons. It was used to generate a genetic map. Forty four AFLPs, 9 RAPDs, 2 isoenzymes and 1 storage protein were mapped onto a previous genetic map consisting… More >

García-Zambrano² Eduardo A, Adriana Gutiérrez-Díez, Gilberto E Salinas-García, Elizabeth Cárdenas-Cerda, Rigoberto E Vázquez-Alvarado, Francisco Zavala-García, Jesús Martínez de la Cerda

Phyton-International Journal of Experimental Botany, Vol.75, pp. 125-135, 2006, DOI:10.32604/phyton.2006.75.125

Abstract The objective of this investigation was to develop and apply molecular markers (type RAPD) for estimating the genetic diversity of 100 Opuntia accessions from the Germoplasma Bank of the FAUANL. The molecular data were evaluated by multivariate analysis using the methods of Ward and UPGMA. These results were compared with phenotypic data taken from the same accessions, already evaluated by the above mentioned methods. The protocol was modified for the extraction of the Opuntia DNA to reduce the quantity of polysaccharides and their influence in its quality. Later on, a protocol was developed for the generation of molecular markers of… More >

García-Zambrano EA¹, MC Ojeda-Zacarías¹, JG Gutiérrez-Soto¹, SR Sinagawa-García¹, A Gutiérrez-Díez¹, J Martínez-de la Cerda¹, JA Pedroza-Flores¹, C Gallegos-Vázquez², JI García-Zambrano³

Phyton-International Journal of Experimental Botany, Vol.87, pp. 87-93, 2018, DOI:10.32604/phyton.2018.87.087

Abstract The technologies based on molecular markers of DNA are the most advanced and possibly the most effective and accurate means to identify genetic variation. The objective of this study was to use molecular genetic markers namely AFLP as a tool for the molecular differentiation of 36 accessions of xoconostle collected in the Centro Regional Universitario Centro Norte of the Universidad Autónoma de Chapingo (CRUCEN-UACh.). DNA extraction was performed by the method of cellular ruptor using FastDNA^® Kit, and quantification of purity and yield of DNA was carried out through the Nanodrop 2000. It was observed that the DNA concentrations ranged… More >