JIANHUA LI1,#, YAOGANG ZHANG1,2,#, TAO ZHANG3, MEIYUAN TIAN1, JING HOU1, DENGLIANG HUANG1, YAN CHENG1,3, ZHU MAN1,3, XIAOMING SU1,3, ZHIQIN LI1, SIXIAN TONG1, XUAN ZHANG1,3, JUN DENG1,3, YUN DONG1,3, YANYAN MA1,2,3,*
BIOCELL, Vol.44, No.2, pp. 209-215, 2020, DOI:10.32604/biocell.2020.08941
Abstract The aim of this study was to find a way to efficiently separate neuronal cells from the cerebral cortex of adult
rats, providing a reference method for rapid acquisition of neuronal cells from the adult rat brain. Fifteen SD rats were
randomly divided into three groups, with five SD rats in each group. Then, neuron cells were isolated from the adult rat
cerebral cortex by the grinding method, the trypsin method, and the collagenase II method, respectively. The expression
of anti-NeuN in the neurons of each group was analyzed by flow cytometry. The acquisition rates and morphology of
neurons of… More >
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