Jianxiong Xu¹, Lu Wang¹, Jinxuan Wang¹, Juhui Qiu^1,*, Guixue Wang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 142-142, 2019, DOI:10.32604/mcb.2019.07273

Abstract Endothelial cell injured or dysfunction, which results lipid deposition and inflammation, is the key point to exacerbate the process of atherosclerosis [1, 2]. Meanwhile oscillatory shear stress is a key factor that results cell dysfunction in vascular disease [3, 4]. Previous research reported that P2Y12 plays a critical role in the development of atherosclerotic lesion through promoting smooth muscle cells migration [5]. As well P2Y12 stimulated the internalization and transendothelial transport of high density lipid. However, whether the P2Y12 induce atherosclerosis through endothelial cell remain elusive. In this study we firstly found P2Y12 were expressed… More >

Yang Wang¹, Shuang Ge¹, Junyang Huang¹, Ruolin Du¹, Tieying Yin¹, Guixue Wang^1,*, Yazhou Wang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 140-141, 2019, DOI:10.32604/mcb.2019.07300

Abstract Zonula occludens-1 (ZO-1) is a peripheral membrane protein belongs to the family of zona occludens proteins and plays an important role as a scaffold protein which cross-links and anchors tight junction (TJ) strand proteins, within the lipid bilayer, to the actin cytoskeleton^[1-2]. Stent implantation is the most effective method in the treatment of cardiovascular disease which always destroy junctions of endothelial cells, the functions of the tight junction were also affected. However, the role of ZO-1 before and after stent implantation has not been fully understood. In this study, the expression of ZO-1 were analyzed by… More >

Junyang Huang¹, Shuang Ge¹, Yang Wang¹, Ruolin Du¹, Yazhou Wang¹, Tieying Yin¹, Guixue Wang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 138-139, 2019, DOI:10.32604/mcb.2019.07305

Abstract Tight junctions are the most apical intercellular junctions of the lateral membrane in endothelial cells, regulating the paracellular material and energy exchange and maintain plasma membrane polarity. Occludin protein is one of the important proteins involved in endothelial tight junctions, and also closely related to the occurrence of atherosclerosis. Therefore, the study of occludin is valuable ^[1]. With the implantation of coronary stents, the integrity of the vascular endothelium is damaged and the local mechanical environment at the stent segment was changed ^[2]. The present study tried to explore the impact of mechanical stimulation after stent… More >

Shuang Ge¹, Ruolin Du¹, Yuhua Huang¹, Guixue Wang¹, Yazhou Wang¹, Tieying Yin^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 136-137, 2019, DOI:10.32604/mcb.2019.07317

Abstract Stent implantation is the most effective method in the treatment of cardiovascular disease which always destroy the integrity of the vascular endothelium and the local mechanical environment at the stent segment was changed, especially the biodegradable stents [1]. In this study, 3D printed biodegradable poly (L-lactic acid) stents were implanted into SD rat abdominal aorta and the endothelialization, intimal hyperplasia, and MGF after stent implantation were studied. Besides, based on the MGF we explored the effects of mechanical stimulation on MGF express in vascular endothelial cells and smooth muscle cells, and also the effects of… More >

Qiqing Zhang^1,2,3,*, Yuan Zhang⁴, Linzhao Wang⁴, Yongzhen Xing⁴

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 134-135, 2019, DOI:10.32604/mcb.2019.07301

Abstract Guided tissue regeneration (GTR) is a technique that selectively guides cells to attach and proliferate towards an injured site to achieve tissue regeneration through a physical barrier membrane. In this review, we presented a brief overview of the development of GTR technology and GTR materials. Nowadays, new technologies such as electrospinning, nanotechnology, controlled release technique, and 3D printing have been introduced into the study of GTR materials. Resorbable membrane as GTR materials are available as alternatives to conventional non-resorbable membranes. Current GTR materials not only act as a physical barrier membrane but also as a More >

Viviane Timmermann¹, Kevin Vincent², Joakim Sundnes¹, Andrew D. McCulloch^2,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 133-133, 2019, DOI:10.32604/mcb.2019.07311

Abstract Heterogeneous mechanical dyskinesis has been implicated in arrhythmogenic phenotypes. Strain-induced perturbations to cardiomyocyte electrophysiology (EP) may trigger arrhythmias via a variety of mechano-electric feedback (MEF) mechanisms. While the role of stretch-activated ionic currents (SACs) has been investigated intensively using computational models, experimental studies have shown that mechanical strain can also trigger intra- and inter-cellular calcium waves. To investigate whether the inherent strain dependence of myofilament calcium affinity may promote arrhythmogenic intra- and inter-cellular calcium waves under conditions of pathologic mechanical heterogeneity, we coupled a mathematical model of excitation-contraction coupling (ECC) in rabbit ventricular myocytes to… More >

Pan Zhang^1,2, Chen Zhang^1,2, Jiyang Han^1,2, Xiru Liu^1,2, Qishan Wang³, Hui Yang^1,2,4,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 132-132, 2019, DOI:10.32604/mcb.2019.06839

Abstract Research in stem cell biology relies on the knowledge of the cell microenvironment in vivo, known as “stem cell niche”, where stem cells are nurtured by the niche signals. Hematopoietic stem cells (HSCs) are capable of continuously generating and maintaining the body’s full immune and hematopoietic systems. In adult, a pool of hematopoietic cells, including HSCs, primarily reside in the bone marrow (BM) niches that plays critical roles on cell fate. Niche supporting cells, cytokines, extracellular matrix proteins and other biochemical cues associated with HSCs behaviors (quiescence, self-renewal, proliferation, differentiation, mobilization, homing, and apoptosis) has… More >

Fan Feng¹, Tingting Xia¹, Runze Zhao¹, Mengyue Wang¹, Li Yang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 131-131, 2019, DOI:10.32604/mcb.2019.07211

Abstract Objective: To study the effects of different substrate stiffness on human hepatocytes using RNA sequencing (RNA-Seq) technology. The stiffness was corresponding to physiology and pathology stiffness of liver tissues.
Results: With the aid of RNA-Seq technology, our study characterizes the transcriptome of hepatocytes cultured on soft, moderate, stiff and plastic substrates. Compared to soft substrate, our RNA-Seq results revealed 1131 genes that were up-regulated and 2534 that were down-regulated on moderate substrate, 1370 genes that were up-regulated and 2677 down-regulated genes on stiff substrate. Functional enrichment analysis indicated that differentially expressed genes were associated with More >

Mengyue Wang¹, Runze Zhao¹, Fan Feng¹, Tingting Xia^1,*, Li Yang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 130-130, 2019, DOI:10.32604/mcb.2019.07154

Abstract Hepatocellular carcinoma (HCC) is the third most common cancer in the world. Previous studies have shown that hard matrix promotes the proliferation of liver tumor cells. However, the role of matrix stiffness on hepatic cancer stem cells (HCSCs) is still unclear. Three-dimensional hydrogels with different stiffness were used to mimic the normal liver tissue (4kPa) and cancerous liver tissue (26kPa) stiffness. The proliferation, stemness and invasion properties of HCSCs under 3D different stiffness were detected. METHOD: HSCSs were screened and cultured by enrichment method, and the effect of matrix stiffness on HCSCs was studied by… More >

Yun Peng¹, Guoan Li^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 128-129, 2019, DOI:10.32604/mcb.2019.07392

Abstract This article has no abstract. More >