Bahram Barati¹, Fatemeh Fazeli Zafar¹, Shuanhu Hu¹, Najmeh Fani², Sajjad Eshtiaghi³, Shuang Wang^1,*

Journal of Renewable Materials, Vol.9, No.11, pp. 1843-1857, 2021, DOI:10.32604/jrm.2021.016301 - 04 June 2021

Abstract Enhancing the stability of biomolecules is one of the hot topics in industry. In this study, we enhanced the stability of an important protein called LEPTIN. LEPTIN is a hormone secreted by fat cells playing an essential role in body weight and composition, and its deficiency can result in several disorders. The treatment of related LEPTIN dysfunctions is often available in the form of injection. To decrease the cost and the frequency of its applications can be achieved by increasing its lifetime through engineering LEPTIN. In this study, to engineer LEPTIN, we have introduced disulfide… More > Graphic Abstract

Ruma Arora Soni^1,*, K. Sudhakar^1,3,4,*, R. S. Rana²

Energy Engineering, Vol.118, No.4, pp. 1045-1056, 2021, DOI:10.32604/EE.2021.016082 - 31 May 2021

Abstract Spirulina is a sort of algae that grows in both fresh and seawater. It is considered the Earth’s most nutritionally dense food. Certain claims about Spirulina’s beneficial health properties are attributed to the relatively high protein content of the cells. Spirulina’s lipid, fatty acid profile, and biochemical composition have received little attention. The purpose of this study is to investigate the nature and decomposition of spirulina biomass at various temperatures. In the present investigation, Fourier transforms infrared spectroscopy, thermogravimetric analysis, and elemental analysis were used to study spirulina biomass biochemical characteristics. The optimal content of… More >

Xin Li, Feiya Liao, Qiqi Ma, Beibei Jiang^*, Yuanzhi Pan, Cheng Luo, Xinjie Wang, Aining Ran

Phyton-International Journal of Experimental Botany, Vol.90, No.5, pp. 1415-1423, 2021, DOI:10.32604/phyton.2021.016143 - 27 May 2021

Abstract Chrysanthemum is one of the most important ornamental flowers in the world, and temperature has a significant influence on its field production. In the present study, differentially expressed proteins were investigated in the leaves of Dendranthema grandiflorum ‘Jinba’ under high temperature stress using label-free quantitative proteomics techniques. The expressed proteins were comparatively identified and analyzed. A total of 1,463 heat-related, differentially expressed proteins were successfully identified by Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS), and 1,463 heat-related, differentially expressed proteins were successfully identified by mass spectrometry after a high temperature treatment. Among these, 701 proteins were upregulated and… More >

Sara Saber¹, Uswah Khairuddin^2,*, Rubiyah Yusof², Ahmed Madani¹

CMC-Computers, Materials & Continua, Vol.68, No.3, pp. 3563-3576, 2021, DOI:10.32604/cmc.2021.017769 - 06 May 2021

Abstract The identification of DNA binding proteins (DNABPs) is considered a major challenge in genome annotation because they are linked to several important applied and research applications of cellular functions e.g., in the study of the biological, biophysical, and biochemical effects of antibiotics, drugs, and steroids on DNA. This paper presents an efficient approach for DNABPs identification based on deep transfer learning, named “DTLM-DBP.” Two transfer learning methods are used in the identification process. The first is based on the pre-trained deep learning model as a feature’s extractor and classifier. Two different pre-trained Convolutional Neural Networks… More >

Xiaoli Zhang^1,2,#, Xiaohong Yin^2,3,#, Jiana Chen^3,#, Fangbo Cao³, Yu Liu³, Zhengwu Xiao³, Liqin Hu³, Guanghui Chen³, Tianfeng Liang^1,2,*, Min Huang^3,*

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1285-1292, 2021, DOI:10.32604/phyton.2021.014778 - 27 April 2021

Abstract Protein in rice grains is an important source of nutrition for rice consumers. This study mainly aimed to identify the critical factors that determine grain protein concentration in rice. Accumulation parameters, including mean accumulation rate (R_mean) and active accumulation duration (D_active), for protein and non-protein components and their correlations with protein concentration in rice grains were investigated in field experiments conducted over two years with six rice cultivars. Results showed that grain protein concentration ranged from 9.6% to 11.9% across cultivars and years. Accumulation processes of protein and non-protein components were well fitted by the logistic More >

LORENZO ALIBARDI¹, BERND NOECKER²

BIOCELL, Vol.45, No.4, pp. 1013-1022, 2021, DOI:10.32604/biocell.2021.013913 - 22 April 2021

Abstract The differentiation of cells composing mature human hairs produces layers with different corneous characteristics that would tend to flake away one from another, as in the corneous layer of the epidermis, without anchoring junctions. It is likely that cell junctions established in the forming cells of the hair bulb are not completely degraded like in the corneous layer of the epidermis but instead remain in the hair shaft to bind mature cuticle, cortex, and medulla cells into a compact hair shaft. During cell differentiation in hairs, cell junctions seem to disappear, and little is known… More >

CHUNKUN FAN, YONGPENG ZHANG, CHUNBAO YANG, YAWEI TANG, JI QU, BU JIE, DEJI QUZHEN, LIYUN GAO^*

BIOCELL, Vol.45, No.4, pp. 1121-1135, 2021, DOI:10.32604/biocell.2021.014336 - 22 April 2021

Abstract Salinity is a serious challenge for agriculture production by limiting the arable land. Rice is a major staple food crop but very sensitive to salt stress. In this study, we used Arabidopsis for the functional characterization of a rice F-box gene LOC_Os04g48270 (OsPP12-A13) under salinity stress. OsPP12-A13 is a nuclear-localized protein that is strongly upregulated under salinity stress in rice and showed the highest expression in the stem, followed by roots and leaves. Two types of transgenic lines for OsPP12-A13 were generated, including constitutive tissue over-expression using the CaMV35S promoter and phloem specific over-expression using the pSUC2 promoter.… More >

Antonio Pizzi^*

Journal of Renewable Materials, Vol.9, No.8, pp. 1345-1364, 2021, DOI:10.32604/jrm.2021.015663 - 08 April 2021

Abstract Collagen powder hydrolysates were reacted with a solution of commercial mimosa bark tannin extract. The mixture was prepared at ambient temperature and prepared at 80°C to determine what reactions, if any, did occur between the collagen protein through its amino acids and the polyphenolic condensed tannin. The reaction products obtained were analyzed by matrix assisted laser desorption ionization time-of-flight (MALDI ToF) mass spectrometry. Reactions between the two materials did appear to occur, with the formation of a relatively small proportion of covalent and ionic linkages at ambient temperature but a considerable proportion of covalent linkages… More >

Xiaohang He¹, Minjie Liu², Zhengwu Fang¹, Dongfang Ma^1,2,3,*, Yilin Zhou^1,3,*, Junliang Yin¹

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 971-986, 2021, DOI:10.32604/phyton.2021.012726 - 30 March 2021

Abstract Plant AT-rich sequence and zinc-binding protein (PLATZ) is a plant transcription factor that has been studied in corn. PLATZ can non-specifically bind to sequences rich in A/T bases to induce transcriptional repression. It is involved in the regulation of dehydration tolerance in seeds. In this study, we performed bioinformatics analysis to identify and characterize wheat PLATZ(TaPLATZ)genes. We identified 49 wheat PLATZ genes by searching the wheat genome by using known PLATZ gene sequences from rice, Arabidopsis, and maize. Phylogenetic analysis on PLATZ gene sequences from different species was performed. We found that PLATZs could be divided into three groups.… More >

D. Venugopal¹, T. Jayasankar^2,*, N. Krishnaraj³, S. Venkatraman⁴, N. B. Prakash⁵, G. R. Hemalakshmi⁵

Intelligent Automation & Soft Computing, Vol.28, No.1, pp. 27-38, 2021, DOI:10.32604/iasc.2021.015205 - 17 March 2021

Abstract Comprehensive evaluation of common complex diseases associated with common gene mutations is currently a hot area of human genome research into causative new developments. A multi-fractal analysis of the genome is performed by placing the entire DNA sequence into smaller fragments and using the chaotic game representation and systematic methods to calculate the general dimensional spectrum of each fragment. This is a time consuming process as it uses floating point to represent large data sets and requires processing time. The proposed Regressive Multi-Array Deep Neural Classifier (RMDNC) system is implemented to reduce the computation time,… More >