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  • Open Access

    ARTICLE

    Integrative Analysis of Genetic-Ecological Factors Shaping Epimedium Chemical Diversity

    Ziying Huang1, Ruikang Ma1, Anning Li2, Yufei Cheng1, Xiaolin Lin2, Mengzhi Li3, Yu Zhang2, Liping Shi1, Linlin Dong1,*

    Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2025.074419 - 30 January 2026

    Abstract Epimedium is commonly used to treat bone injury and kidney disease, with prenylated flavonol glycosides (PFGs) as its active ingredients. It has attracted much attention due to prominent healthcare and therapeutic effects, but faces problems of adulteration with closely related species and confusion about geographical origins. In this study, multiple technical approaches were employed to identify its genetic characteristics and metabolic differences. Based on DNA barcoding, 20 batches of samples were analyzed. The genetic distances of matK, ITS and psbA-trnH within species were all smaller than those between species, and psbA-trnH along with ITS + psbA-trnH proved most effective… More >

  • Open Access

    ARTICLE

    Prediction of Root Zone Temperature Dynamics at Effective Depth on Lettuce Production in Greenhouse Using Sensitivity and Feature Importance Analysis with XGBoost

    Hasan Kaan Kucukerdem*

    Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2026.074188 - 30 January 2026

    Abstract Root-zone temperature (RZT) strongly affects plant growth, nutrient uptake and tolerance to environmental stress, making its regulation a key challenge in greenhouse cultivation in cold climates. This study aimed to assess the potential of passive techniques, namely black polyethylene mulch and row covers, for modifying RZT dynamics in lettuce (Lactuca sativa L.) production and to evaluate the predictive performance of the eXtreme Gradient Boosting (XGBoost) algorithm. Experiments were conducted in Iğdır, Türkiye, over a 61-day period, with soil temperature continuously monitored at depths of 1–30 cm under mulched and non-mulched conditions, alongside measurements of greenhouse air… More >

  • Open Access

    ARTICLE

    Growth and Yield Responses of Soledad Chili Pepper (Capsicum annuum L.) to the Application of Chitosan and Bacillus subtilis

    Adolfo Amador Mendoza1,*, Rosalba Guadalupe Gomez Raymundo2, Ana Rosa Ramírez Seañez1, Hipolito Hernández Hernández1, Rogelio Enrrique Palacios Torres1, Nelda Xanath Martínez Galero3, Miguel Ángel García Muñoz3, Saribel Zilli Gutiérrez4,*

    Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2025.073856 - 30 January 2026

    Abstract The cultivation of Soledad pepper (Capsicum annuum L.) is essential in Oaxaca and Veracruz, but it faces issues with pests and diseases, which affect yield and cause economic losses. To mitigate these impacts, farmers have started using biostimulants such as chitosan and plant growth promoting bacteria instead of agrochemicals due to their environmental and health benefits. This study evaluated the effect of Bacillus subtilis and chitosan, both individually and combined, on the growth, yield, and fruit quality of Soledad pepper under greenhouse conditions. Four treatments were applied at different stages of the crop cycle: Q (Chitosan), BS (Bacillus More >

  • Open Access

    ARTICLE

    PpCSC1, a Novel ERD4 Ortholog from Physcomitrium patens, Plays a Negative Role in Salt Stress Tolerance

    Lu Chen1,2,#, Zhijie Ren2,#, Guangmin Zhao2, Xuan He2, Legong Li2, Sheng Teng1,*, Yikun He2,*, Fang Bao2,*

    Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2025.073817 - 30 January 2026

    Abstract ERD4 proteins, members of the early responsive-to-dehydration family, act as plasma membrane ion channels that contribute to ion homeostasis and modulate plant response to abiotic stresses. However, the functions of ERD4 homologs in non-vascular species remain largely unexplored. Here, we characterized an ERD4 family homolog in Physcomitrium patens (Hedw.) Mitt., PpCSC1 (Calcium-permeable Stress-responsive Cation Channel 1), and investigated its role in salt stress response. PpCSC1 localized to the plasma membrane and functioned as a non-selective cation channel permeable to Na+, K+, Ca2+, and Mg2+. Under salt treatment, PpCSC1 transcripts were markedly downregulated, whereas overexpression lines exhibited enhanced salt sensitivity.… More >

  • Open Access

    ARTICLE

    Physiological and Metabolic Responses of Red Leaf Lettuce (Lactuca sativa L.) under Low Pressure Conditions

    Wonkyu Yi, Jongseok Park*

    Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2026.073450 - 30 January 2026

    Abstract Understanding plant responses under low-pressure conditions is important for developing closed cultivation systems that simulate space environments. This study aimed to assess the effects of different pressure levels on growth, photosynthesis, and secondary metabolite accumulation in red leaf lettuce (Lactuca sativa L. var. ‘Super Caesar’s Red’). Plants were cultivated for three weeks in sealed chambers under 101 kPa (atmospheric pressure), 66 kPa (moderate low pressure), and 33 kPa (severe low pressure). Growth analysis showed that leaf length and leaf area decreased significantly with reduced pressure, while chlorophyll content and SPAD values increased gradually. Photosynthetic measurements indicated More >

  • Open Access

    ARTICLE

    Comparative Analysis of the Impact of Different Ecotypes on In Vitro Anti-Inflammatory Activity of Ethanolic Extracts of Moringa oleifera Leaves

    Mario D’Ambrosio1, Elisabetta Bigagli1,*, Lorenzo Cinci1, Cecilia Brunetti2,*, Edgardo Giordani3, Francesco Ferrini3, Cristina Luceri1

    Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2026.073250 - 30 January 2026

    Abstract Moringa oleifera (MO) is traditionally used to mitigate inflammatory-mediated disorders; however, the influence of ecotypic variation on its anti-inflammatory activity remains poorly understood. In this study, we compared the phytochemical composition and anti-inflammatory activity of ethanolic extracts obtained from fresh and dried leaves of four MO ecotypes (India, Paraguay, Mozambique, and Pakistan), all grown under the same outdoor conditions, as well as two commercial powders (Just Moringa and WISSA), using LPS-stimulated RAW 264.7 macrophages. Extracts from fresh leaves were 19–43% more cytotoxic than those from dried leaves, depending on the ecotype, likely due to higher cyanogenic… More >

  • Open Access

    ARTICLE

    Tissue-Specific Transcriptomic Responses and Viral Accumulation in Lily Cultivars Infected with Cucumber Mosaic Virus

    Yun-Im Kang1, Youn Jung Choi1, Su Young Lee1, Young-Ran Lee1, Ki-Byung Lim2,3, Yun-Jae Ahn2,3,*

    Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2026.073138 - 30 January 2026

    Abstract Cucumber mosaic virus (CMV) threatens lily production by reducing floral quality and enabling carry-over via infected planting stock. To explore tissue-specific host responses, we analyzed a legacy, single-replicate RNA-seq dataset from two cultivars, ‘Cancun’ and ‘Connecticut King’ (CK), profiling leaf (source) and bulb (sink) tissues at 0 and 28 days post-inoculation (dpi), alongside leaf DAS-ELISA. Principal component analysis indicated that tissue identity dominated the transcriptome (PC1 = 47.7%), with CMV treatment driving within-tissue shifts over time. Exploratory Gene Ontology/KEGG summaries and a focused marker panel revealed a consistent split: in leaves, genes linked to jasmonate/WRKY-associated… More >

  • Open Access

    ARTICLE

    Impacts of Fertilization and Soil Amendments on Rhizosphere Microbiota and Growth of Panax: A Meta-Analysis

    Hong Chen1,2, Runze Yang1,2, Jing Tian1,2, Boyuan Xu1,2, Qiang Chen3, Yuzong Chen1,2, Ming-Xiao Zhao1,2,*

    Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2025.072276 - 30 January 2026

    Abstract Panax species are globally recognized for their high medicinal and economic value, yet large-scale cultivation is constrained by high production costs, progressive soil acidification, and persistent soil-borne diseases. Although various soil improvement strategies have been tested, a comprehensive synthesis of their comparative effectiveness has been lacking. Here, we conducted a meta-analysis of 1381 observations from 54 independent studies to evaluate the effects of conventional fertilizers, microbial fertilizers, organic amendments, and inorganic amendments on Panax cultivation. Our results demonstrate that microbial fertilizers, organic amendments, and inorganic amendments significantly increased soil pH, thereby ameliorating soil acidification. Among them,… More >

  • Open Access

    REVIEW

    Mental Health and Well-Being of Doctoral Students: A Systematic Review

    Yuxin Guo1,2, Xinqiao Liu3,*

    International Journal of Mental Health Promotion, Vol.28, No.1, 2026, DOI:10.32604/ijmhp.2025.074063 - 28 January 2026

    Abstract Background: Mental health concerns among doctoral students have become increasingly prominent, with consistently low levels of well-being making this issue a critical focus in higher education research. This study aims to synthesize existing evidence on the mental health and well-being of doctoral students and to identify key factors and intervention strategies reported in the literature. Methods: A systematic review was conducted to examine the determinants and interventions related to doctoral students’ mental health and well-being. Relevant studies were comprehensively searched in Web of Science, PubMed, Scopus, and EBSCO, with the final search conducted on September 19,… More >

  • Open Access

    ARTICLE

    The Relationship among Chinese Teachers’ Organizational Support, Career Adaptability and Job Satisfaction: The Mediating Effect of Decent Work

    Huaruo Chen1,2, Gefan Wang1, Hancai Qiu1, Hui Ma1, Zhentao Peng1, Ruihan Liu3, Feng Xu4,*

    International Journal of Mental Health Promotion, Vol.28, No.1, 2026, DOI:10.32604/ijmhp.2025.073911 - 28 January 2026

    Abstract Background: As an important indicator of subjective well-being (SWB), decent work is a key guarantee for the sustainable development of teachers and their psychological health and work quality. Faced with the rapid development of artificial intelligence and the global labor market, vocational college teachers are facing challenges such as workload pressure and limited career development, which may harm their well-being. This study aims to localize the measurement method of decent work in Chinese vocational education based on the theory of the Psychology of Working Theory, and explore the relationship mechanism between organizational support, career adaptability, decent… More >

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