LONGSHENG FU¹, JINFANG HU¹, FENG SHAO², YAOQI WU¹, WEI BAI³, MINGJIN JIANG³, HAO CHEN⁴, LIHUA CHEN², YANNI LV^1,*

BIOCELL, Vol.47, No.8, pp. 1869-1883, 2023, DOI:10.32604/biocell.2023.028602

Abstract Background: Biochanin A is an excellent dietary isoflavone that has the concomitant function of both medicine and foodstuff. The attenuation function of biochanin A on blood-brain barrier (BBB) damage induced by cerebral ischemia-reperfusion remains unclear. Methods: C57BL/6 mice were subjected to 1 h middle cerebral artery occlusion (MCAO) followed by 24 h reperfusion. The infarct volume of the brain was stained by TTC, while leakage of the brain was quantitatively stained by Evans blue, and the neurologic deficit score was measured. Microglial-induced morphologic changes were observed via immunofluorescence staining, and rolling and adhering leukocytes in venules were observed via two-photon… More >

MIN WANG^1,#, HAILIANG ZHAO^1,2,#, WEIWEI CHEN^3,#, CAIQUN BIE^4,#, JINYING YANG¹, WENRUI CAI¹, CHUTIAN WU¹, YANFANG CHEN¹, SHUFEN FENG¹, YING SHI¹, YUTING LI¹, HUIJUN TANG⁴, LIXIAN ZHONG¹, LILIANGZI GUO¹, SISI CHEN¹, LINJING LONG⁵, SHAOHUI TANG^1,*

Oncology Research, Vol.31, No.4, pp. 515-541, 2023, DOI:10.32604/or.2023.029101

Abstract The dysregulation of exosomal microRNAs (miRNAs) plays a crucial role in the development and progression of cancer. This study investigated the role of a newly identified serum exosomal miRNA miR-4256 in gastric cancer (GC) and the underlying mechanisms. The differentially expressed miRNAs were firstly identified in serum exosomes of GC patients and healthy individuals using next-generation sequencing and bioinformatics. Next, the expression of serum exosomal miR-4256 was analyzed in GC cells and GC tissues, and the role of miR-4256 in GC was investigated by in vitro and in vivo experiments. Then, the effect of miR-4256 on its downstream target genes… More > Graphic Abstract

FEI TIAN^*, GANG LIU, LINLIN FAN, ZHONGYUN CHEN, YAN LIANG,

BIOCELL, Vol.45, No.1, pp. 49-56, 2021, DOI:10.32604/biocell.2021.013794

Abstract Cerebral ischemia is due to the formation of blood clots or embolisms in the brain arteries, which leads to local brain tissue necrosis and neural cell apoptosis. Recent studies have shown that microRNA (miRNA) plays an important regulatory role in the pathological process of ischemic injury. The aim of this study is to investigate the role and the mechanism of miR-21-3p and drosophila mothers against decapentaplegic 2 (SMAD2) in cerebral ischemic reperfusion injured (CIRI) neural cells. The CIRI model was established by oxygen-glucose deprivation and recovery process for N2a cells. The cell viability and the apoptotic was evaluated by MTT… More >

Molecular & Cellular Biomechanics, Vol.15, No.1, pp. 21-36, 2018, DOI:10.3970/mcb.2018.015.021

Abstract Dendritic cells (DCs) are the most potent professional antigen presenting cells as now known, which play critical roles in the initiation, programming and regulation of the immune response. Transforming growth factor-β₁ (TGF-β₁), one of the major suppressive cytokines in tumor microenvironment, can deteriorate the biomechanical characteristics and motility of mature dendritic cells (mDCs)，but the underlying molecular mechanisms are not well defined. In this study, the effects of TGF-β₁ on the motilities and T cell priming capabilities of mDCs as well as the molecular regulatory mechanisms were investigated. The results showed that the cytoskeleton (F-actin) organizations of mDCs were abnormally remodeled… More >