KSENIA S. STAFEEVA¹, NATALIA A. SAMOYLOVA¹, OLGA A. KARANDEEVA¹, VERONIKA V. NESTEROVA¹, KIRILL A. STARODUBTSEV¹, EVGENY V. MIKHAILOV², ILYA O. KRUTOV², EVGENY S. POPOV³, NATALIA S. RODIONOVA⁴, ANASTASIA V. KOKINA^1,5, ARTEM P. GUREEV^1,5,*

BIOCELL, Vol.49, No.1, pp. 7-20, 2025, DOI:10.32604/biocell.2024.058339 - 24 January 2025

Abstract Objective: The objective of this study was to determine the level of methotrexate (MTX) toxicity in the intestines of mice and to evaluate the protective effect of probiotics composed of Streptococcus, Bifidobacterium, and Lactobacillus species on intestinal cells during MTX treatment. Methods: Mice were divided into three groups: control, MTX group (received MTX injections), and MTX + probiotics group (received MTX injections along with a diet containing probiotics). Morphological and histological changes, the level of mitochondrial DNA (mtDNA) damage, the level of lipid peroxidation products, and gene expression in the mice’s small intestine were assessed. Results: We… More > Graphic Abstract

Odin RAMÍREZ-FERNÁNDEZ^1,2,3,*, Ludovika CACOPARDO¹, Benjamín LEON-MANCILLA², Joana COSTA¹

BIOCELL, Vol.43, No.1, pp. 29-36, 2019, DOI:10.32604/biocell.2019.04790

Abstract We present a bioreactor system which combines a semi permeable membrane that simulates the osmotic nutrients interchange in the small intestine circulation and rhythmic peristaltic movement. This custom-designed presents a semipermeable membrane bioreactor, with peristaltic flow and compression variation that allows adjustment of luminal flow rate. In addition, this system is also capable of achieving the drug distribution in the small intestine model from the apical compartment to the basal compartment by the semipermeable channel. This dynamic bioreactor can mimic the human small intestine with increased accuracy to overcome many of the limitations and accuracy More >

OSCAR MAC DONAL¹, JUAN G. CHEDIACK^1,2,3, ENRIQUE CAVIEDES-VIDAL^1,2,3

BIOCELL, Vol.32, No.3, pp. 219-227, 2008, DOI:10.32604/biocell.2008.32.219

Abstract The isolation of viable enterocytes, villi and crypts from the small intestine of a feral bird (Columba livia) is important for performing physiological experiments in ecologically relevant processes of membrane transport. The effectiveness of mechanical disruption, enzymatic digestion and chelating agents were compared. The objectives were to isolate enterocytes, villi and crypts from the small intestine of young pigeons; to evaluate the viability of the isolated intestinal epithelial cells isolated; and to verify the integrity of enterocytes by biochemical features. Enzymatic and mechanical methods yielded both elongated columnar and spherical cells. With the chelating method villi More >

MARIA RAQUEL MARÇAL NATALI, SONIA LUCY MOLINARI, LUIZ CRISTIANO VALENTINI, MARCÍLIO HUBNER DE MIRANDA NETO.

BIOCELL, Vol.29, No.1, pp. 39-46, 2005, DOI:10.32604/biocell.2005.29.039

Abstract The purpose of this work was to analyze the morphoquantitative behavior of the neurons of the myenteric plexus, as well as the morphometry of the duodenal wall, in adult rats fed with normoproteic (22%) and hypoproteic (8%) rations, killed at the age of 345 days. For neuronal assessments duodenal wholemounts stained with the Giemsa method were used, and for the evaluation of the duodenal wall routine histological processing and staining with Hematoxilin-Eosin were employed. The means of the number of neurons in 80 microscopic fields (12.72 mm² ) and of the size of the neuronal cell More >

LUZMARINA HERNANDES*, LUCIENI CRISTINA MARQUES DA SILVA PEREIRA*, ELIANA PARISI ALVARES**

BIOCELL, Vol.27, No.3, pp. 347-351, 2003, DOI:10.32604/biocell.2003.27.347

Abstract The enteric nervous system plays a role on the stimulation of secretory cells of intestinal epithelia. We have demonstrated that ablation of ENS stimulates epithelial cell proliferation. As goblet cells are important constituents of the epithelial sheet, it is mandatory to investigate separately this cell type. The myenteric plexus of the ileum of rats in postnatal development was partially removed by the serosal application of benzalkonium chloride (BAC). Three groups of animals were used: those where BAC application was at 13 days and sacrifice was at 15 (13/28-day-old) or 23 days after treatment (13/36-day-old), and More >