CARLOS ALBERTO-SILVA^1,*, ANA CAROLINA DE ARAUJO¹, RODRIGO SIMãO BONFIM¹, JOYCE MEIRE GILIO²

BIOCELL, Vol.47, No.2, pp. 289-295, 2023, DOI:10.32604/biocell.2023.023787

Abstract Bothrops envenomation is complex and provokes prominent local tissue damage and systemic disturbances, but little is known about their effects on the male reproductive system. After intratesticular injection, the bioactive peptide fraction (Bj-PF) obtained from Bothrops jararaca snake venom changes the structure of different stages of the seminiferous epithelium cycle in adult mice. For the first time, we investigated whether local envenomation of Bj-PF induces toxicological effects on the male reproductive system, particularly on the seminiferous epithelium and Sertoli cells. Male adult mice were treated with 0.24 mg.kg⁻¹ by intramuscular (i.m.) injection for 24 h. The testes samples were collected… More >

S.L. MARUÑAK^*, L. LEIVA^**, M.E. GARCIA DENEGRI^*, P. TEIBLER^*, O. ACOSTA DE PÉREZ^*

BIOCELL, Vol.31, No.3, pp. 355-364, 2007, DOI:10.32604/biocell.2007.31.355

Abstract A phospholipase A₂ has been isolated from Bothrops jararacussu venom from snakes that inhabit the northeast region of Argentina. The present study describes in vivo and in vitro biological activities of phospholipase A₂ from B. jararacussu as well as isolation details of its. Venom was obtained by milking of adult snakes which were housing in wood reptile cages of varying dimensions in heated (20–30ºC) rooms. Snakes received a weekly diet of mice and water was available ad libitum for drinking and soaking. The enzyme was purified by gel filtration on a Sephadex G-75 column followed by ion exchange chromatography on… More >