LIJUAN ZHANG^1,#, QINGYIN MENG^2,#, LI ZHUANG¹, QUAN GONG¹, XIANDA HUANG³, XUEQIN LI¹, SHIJUAN LI¹, GUOQIN WANG⁴, XICAI WANG^5,*

BIOCELL, Vol.48, No.4, pp. 581-590, 2024, DOI:10.32604/biocell.2024.047260

Abstract Background: Lung adenocarcinoma is a very pervasive histological form of lung cancers, and inhibiting metastasis is crucial for effective treatment. In this investigation, we explored the functional interaction of miR-30a-5p and the putative transcription factor 2 of the homeodomain (PHTF2) in dictating the aggressiveness and metastasis of lung adenocarcinoma. Method: We collected clinical samples to evaluate the expression patterns of miR-30a-5p and PHTF2 in lung adenocarcinoma along with normal tissues. Cellular experiments including cell count kit (CCK)-8 growth assay, apoptosis analysis, migration and invasion examinations were performed to assess the aggressiveness of lung adenocarcinoma cells. Furthermore, we examined tumorigenesis and… More > Graphic Abstract

GANG LIU^1,#, LEI SHI^1,#, BIN WANG¹, ZEHUI WU¹, HAIYUAN ZHAO¹, TIANYU ZHAO², LIANGHUI SHI^1,*

Oncology Research, Vol.32, No.3, pp. 585-596, 2024, DOI:10.32604/or.2023.029349

Abstract The role of lncRNA KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1) in colon cancer involves various tumorigenic processes and has been studied widely. However, the mechanism by which it promotes colon cancer remains unclear. Retroviral vector pSEB61 was retrofitted in established HCT116-siKCN and SW480-siKCN cells to silence KCNQ1OT1. Cellular proliferation was measured using CCK8 assay, and flow cytometry (FCM) detected cell cycle changes. RNA sequencing (RNA-Seq) analysis showed differentially expressed genes (DEGs). Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were carried out to analyze enriched functions and signaling pathways. RT-qPCR, immunofluorescence, and western blotting… More >

YUE XIAO^1,2,#, WENJING MA^2,#, XINYI CHEN², WEIWEI HU³, QIANQIAN DI², XIBAO ZHAO², GUODONG HUANG¹, WEILIN CHEN^1,2,*

BIOCELL, Vol.47, No.12, pp. 2617-2625, 2023, DOI:10.32604/biocell.2023.042476

Abstract Background: Glioma is the most common primary brain tumor. Exploration of new tumorigenesis mechanism of glioma is critical to determine more effective treatment targets as well as to develop effective prognosis methods that can enhance the treatment efficacy. We previously demonstrated that the deubiquitinase biquitin carboxyl-terminal hydrolase L5 (UCHL5) was downregulated in human glioma. However, the effect and mechanism of UCHL5 on the proliferation of glioma cells remains unknown. Methods: Transfection of siRNA was used to knockdown the expression of UCHL5 in U251 cells. The 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay, Edu assay, and colony formation assay were employed to… More > Graphic Abstract

HUI LI¹, CHENGFANG ZHOU², MEI KUANG², YUN LIU^1,*, JIEPING CHEN^1,2,*

BIOCELL, Vol.47, No.10, pp. 2177-2186, 2023, DOI:10.32604/biocell.2023.029373

Abstract Fizzy-related protein homolog 1 (FZR1) mainly functions as a specific activator of the anaphase-promoting complex/cyclosome (APC/C) in the cell cycle and controls the G0 and G1 phases of the cell cycle. We highlight recent work that has studied the role of FZR1 in tumorigenesis, growth, differentiation, and genome stability through cell-cycle control. We summarize the current state of knowledge regarding FZR1 structure, function, and the distinct ways of APC/C dysregulation in solid tumors and hematologic malignancies. We also discuss novel approaches for targeting the FZR1 as a cancer therapy and research area for future work. More >

Linglan Gu, Yi Shi, Weimin Xu, Yangyang Ji

Oncology Research, Vol.27, No.8, pp. 923-933, 2019, DOI:10.3727/096504019X15518706875814

Abstract In previous investigations, we reported that peroxisome proliferator-activated receptor / (PPAR / ) activation by GW501516 inhibits proliferation and promotes apoptosis in the undifferentiated C666-1 nasopharyngeal carcinoma (NPC) cells by modulating caspase-dependent apoptotic pathway. In the present study, the mechanism by which GW501516 induces apoptosis was explored from the perspective of microRNA (miRNA) expression. Among the assayed miRNAs that were involved in regulating the expression of antiapoptotic protein Bcl-2, miR-206 was increased significantly and specifically by GW501516 in C666-1 cells at both the in vitro level and at the in vivo xenograft samples. The induction on miR-206 expression caused by… More >

Zhen Li^*1, Xin Li^*1, Xiao Du^†, Henghui Zhang^†, Zhengyang Wu^*, Kewei Ren^*, Xinwei Han^*

Oncology Research, Vol.27, No.6, pp. 663-672, 2019, DOI:10.3727/096504018X15420741307616

Abstract Cholangiocarcinoma (CCA) is the second most common primary hepatobiliary carcinoma. The long noncoding RNA (lncRNA) small nucleolar RNA host gene 1 (SNHG1) has been reported to contribute to the progression of multiple cancers. Nonetheless, the functions and hidden mechanism of SNHG1 remain unclear in CCA. In this study, the SNHG1 levels were boosted in CCA cell lines, and knockdown of SNHG1 repressed CCA cell proliferation and invasion in vitro. The data also demonstrated that miR-140 could act as a target of SNHG1 in CCA and inhibited CCA cell proliferation and invasion, whereas the inhibition effects were relieved by overexpression of… More >

Feng Shuai^*, Bo Wang^†, Shuxiao Dong^‡

Oncology Research, Vol.28, No.9, pp. 977-977, 2020, DOI:10.3727/096504021X16297183140281

Abstract This article has no abstract. More >

Hai-Feng Zeng^*, Hai-Yan Qiu^†, Fa-Bo Feng^*

Oncology Research, Vol.29, No.1, pp. 75-75, 2021, DOI:10.3727/096504022X16420719767471

Abstract This article has no abstract. More >

DANIEL WAI-HUNG HO^1,2,#, XUEYING LYU^1,2,#, IRENE OI-LIN NG^1,2,*

BIOCELL, Vol.45, No.6, pp. 1495-1500, 2021, DOI:10.32604/biocell.2021.017227

Abstract Oncovirus infection is crucial in human malignancies. Certain oncoviruses can lead to structural variations in the human genome known as viral genomic integration, which can contribute to tumorigenesis. Existing viral integration detection tools differ in their underlying algorithms pinpointing different aspects or features of viral integration phenomenon. We discuss about major procedures in performing viral integration detection. More importantly, we provide a technical update on Virus-Clip to facilitate its usage on the latest human genome builds (hg19 and hg38) and the adoption of multi-thread mode for faster initial read alignment. By comparing the execution of Virus-Clip using single-thread and multi-thread… More >

WEIGAO ZHONG^1,*, AIQIN CHEN², XIAOHONG TANG¹, YI LIU¹

BIOCELL, Vol.45, No.4, pp. 901-910, 2021, DOI:10.32604/biocell.2021.08672

Abstract Earlier studies indicated that circular RNAs (circRNAs) were found in various cancer cells, and circFOXM1 was reported to act as an oncogene in non-small cell lung cancer (NSCLC). However, the function of circFOXM1 in NSCLC remains unclear. The expression levels of genes were measured using quantitative real-time polymerase chain reactions (qRT-PCR). Cell proliferation and apoptosis were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide solution (MTT) and flow cytometry assay. The relative protein expression was assessed by western blot. Moreover, transwell assays were employed to examine cell migration and invasion. The targeted relationship was confirmed by dual-luciferase reporter assay. The expression of circFOXM1 was… More >