Xiaotong Wei1,#, Xuhong Fan2,#, Xuan Lu1, Liyuan Han1, Honglin Zhang3, Siyan Liu3, Jing Qu3, Shuyan Guan3,*, Yiyong Ma3,*
Phyton-International Journal of Experimental Botany, Vol.91, No.9, pp. 1945-1957, 2022, DOI:10.32604/phyton.2022.020062
Abstract
The embryos of maize (Zea mays L.) inbred lines GS02, GS07, GS08, GS11 and GS15 were used as receptor materials to optimize the receptor system from the aspects of genotype, medium components and stress (PEG6000, mannitol, salt and low phosphorus). The results showed that GS07 had the highest induction rate (95.2%). Orthogonal test analysis showed that the best combination of medium components in induction was A2B3C1D3(2), namely, the concentration of 2, 4-dichlorophenoxy acetic acid (2,4-D) was 4 mg·mL-1, the concentration of L-Proline (L-Pro) was 0.8 mg·mL-1, and the concentration of silver nitrate (AgNO3) was 10 mg·mL-1 (or 5 mg·mL). Interestingly,… More >
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