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  • Open Access

    ARTICLE

    Chemical Composition and Antifungal Efficacy of Mentha rotundifolia Essential Oil against Fusarium oxysporum f. sp. albedinis in Date Palm: Valorisation of Plant Biomass for Natural Antifungal Agents

    Hafida Khelafi1, Wassima Lakhdari2, Mustapha Mounir Bouhenna3, Said Boudeffeur4, Hayet Meamiche1, Salah Neghmouche Nacer5,*, Meriam Laouar6

    Phyton-International Journal of Experimental Botany, Vol.94, No.12, pp. 3975-3989, 2025, DOI:10.32604/phyton.2025.073210 - 29 December 2025

    Abstract Essential oils (EOs) derived from medicinal plants are gaining recognition as sustainable alternatives to synthetic fungicides in the management of plant pathogens. This study investigates the chemical composition, chromatographic profile, and antifungal of Mentha rotundifolia essential oil against Fusarium oxysporum f. sp. albedinis (Foa), the pathogen responsible for Bayoud disease in date palm. The oil was extracted through hydrodistillation and characterized using thin-layer chromatography (TLC) and gas chromatography–mass spectrometry (GC-MS), revealing multiple fractions corresponding to terpenoid constituents and 23 chemical constituents, predominantly oxygenated monoterpenes (68.51%), with piperitenone oxide as the major component (62.53%). The antifungal efficacy was evaluated… More >

  • Open Access

    ARTICLE

    Acalypha gaumeri: Antifungal Activity of Three Populations under Edaphic and Seasonal Variations and Ex-Situ Propagation

    Arely A. Vargas-Díaz1, Daisy Pérez-Brito2, Beatriz Hernández-Carlos3, Jairo Cristóbal-Alejo4,*, Silvia Andrade-Canto2, Marcela Gamboa-Angulo2,*

    Phyton-International Journal of Experimental Botany, Vol.94, No.9, pp. 2839-2853, 2025, DOI:10.32604/phyton.2025.066682 - 30 September 2025

    Abstract In the search for new alternatives to control tropical fungal pathogens, the ethanol extracts (EEs) from Acalypha gaumeri (Euphorbiaceae) roots showed antifungal properties against several tropical fungal phytopathogens. A. gaumeri is classified as endemic to the Yucatan Peninsula, Mexico, an area with distinct rainy, drought and northern seasons. The present study evaluated the antifungal activity of three wild populations of A. gaumeri collected quarterly in different seasons during one year against Alternaria chrysanthemi, Colletotrichum gloeosporioides, and Pseudocercospora fijiensis and explored their ex-situ propagation. The highest activity was shown by the EE from the Tinum wild population during the rainy season against A. chrysanthemi,… More >

  • Open Access

    ARTICLE

    Encapsulation of Clove Oil Nanoemlusion in Chitosan-Based Nano-Composite: In Vitro and in Vivo Antifungal Activity against Rhizoctonia solani and Sclerotium rolfsii

    Ahmed Mahmoud Ismail1,2,3,*, Eman Said Elshewy3, Isra H. Ali4,5, Naglaa Abd Elbaki Sallam Muhanna3, Eman Yehia Khafagi3

    Phyton-International Journal of Experimental Botany, Vol.93, No.11, pp. 2787-2811, 2024, DOI:10.32604/phyton.2024.057518 - 30 November 2024

    Abstract Rhizoctonia solani Kühn and Sclerotium rolfsii Sacc. are the primary soil-borne plant diseases responsible for significant reductions in global crop yields. The primary goal of this study was to investigate the antifungal potentials of clove essential oil (CEO), nanoemulsion form (CEONE) and chitosan/nanoemulsion nanocomposite (CS/CEONE) against R. solani and S. rolfsii through in vitro and in vivo trials. Both CEONE and CS/CEONE were prepared and investigated for their physical chemical and morphological characterization. The poisoned medium method was utilized to evaluate the inhibitory effects of CEO, CEONE and CS/CEONE on the mycelial growth and enzymatic activity of R. solani and S. rolfsii. The… More >

  • Open Access

    ARTICLE

    Development of Molecular Marker Linked with Cercospora Leaf Spot (CLS) Disease Resistance in Vigna radiata, Cloning, and Expression for Evaluating Antifungal Activity against Cercospora canescens

    Maria Babar1, Siddra Ijaz1,*, Imran Ul Haq2, Muhammad Sarwar Khan1

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1289-1300, 2023, DOI:10.32604/phyton.2023.026469 - 06 January 2023

    Abstract We developed a molecular marker for MAS of mungbean resistant varieties against CLS from the consensus sequence (MB-CLsRG) of identified RGAs (MB-ClsRCaG1 and MB-ClsRCaG2). The MB-CLsRG sequence-specific primer pair was used to screen Cercospora leaf spot (CLS) resistant varieties of mungbean in genomic analysis that showed congruency with phenotypic screening. Validation of molecular marker linkage with CLS resistance was performed using rtPCR in transcriptomic analysis. The sequenced PCR products showed 100% homology with MB-CLsRG sequence and putative disease resistance proteins that confirmed the linkage of molecular marker with CLS resistance in mungbean. The antifungal potential of… More >

  • Open Access

    ARTICLE

    Antifungal Activity of Crude Extracts of Tectona grandis L.f. against Wood Decay Fungi

    Enrique Cárcamo-Ibarra1, Mauro Manuel Martínez-Pacheco2, Abril Munro-Rojas1, Jorge Enrique Ambriz-Parra1, Crisanto Velázquez-Becerra1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.8, pp. 1795-1808, 2022, DOI:10.32604/phyton.2022.020578 - 14 April 2022

    Abstract Wood is mainly made up of cellulose, hemicelluloses, lignin polymers and other organic and inorganic substances, making it susceptible to deteriorate by various biological agents. Tectona grandis L.f. (Teak) is a timber species with high resistance to biological deterioration, valued for its durability, beauty, and mechanical resistance. The purpose of this work was to evaluate the antifungal activity of crude extracts from teak on various fungi that cause wood deterioration. For this, Teak heartwood was obtained, then fragmented and pulverized until obtaining a flour which was used for compounds extraction using the Soxhlet technique coupled to… More >

  • Open Access

    ARTICLE

    Taguchi Design of the Experimental approach to Increase the Biomass and Cell Wall Chitosan Contents of Zygomycetous Dimorphic Fungus Benjaminiella Poitrasii

    S.R. MANE1,2, E. K. PATHAN3, G. PATIL4, S. G. TUPE5, V. GHORMADE4, B. P. CHAUDHARI1,2, M.V. DESHPANDE5,*

    Journal of Polymer Materials, Vol.38, No.3-4, pp. 219-230, 2021, DOI:10.32381/JPM.2021.38.3-4.4

    Abstract A dimorphic fungus Benjaminiella poitrasii contains high chitin/chitosan (35% of the cell wall) in the mycelial (M) form than its yeast (Y) form (20% of the cell wall). However, the relative proportion of chitosan is more in yeast form cells (chitosan: chitin ratio, 6:1) than mycelial cells (chitosan: chitin ratio, 3:1). Using the Taguchi design of experimental (DOE) approach, interactions among eight different parameters showed that carbon source (starch, 10 g/L), incubation time (48 h), inoculum (M and Y mixed 10%), yeast extract (6 g/L) and peptone (10 g/L), were optimum for maximum biomass production.… More >

  • Open Access

    ARTICLE

    Green Synthesis of Silver Nanoparticles from Abronia villosa as an Alternative to Control of Pathogenic Microorganisms

    Ali Abdelmoteleb1, Benjamin Valdez-Salas2, Ernesto Beltran-Partida2, Daniel Gonzalez-Mendoza3,*

    Journal of Renewable Materials, Vol.8, No.1, pp. 69-78, 2020, DOI:10.32604/jrm.2020.08334 - 01 January 2020

    Abstract The aim of this study was to evaluate the antibacterial and antifungal activities of eco-friendly synthesized silver nanoparticles. The silver nanoparticles were synthesized by biological method using aqueous extract of Abronia villosa. Synthesis of silver nanoparticles was confirmed by color change and characterized using UV-visible spectroscopy, scanning electron microscope (SEM), energy dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), and zeta potential analysis. The SEM analysis revealed the presence of spherical silver nanoparticles of the size range 21 to 33 nm. Synthesized silver nanoparticles were used to evaluate their antibacterial effects at different concentrations (25, 50, More >

  • Open Access

    ARTICLE

    Antifungal Activity of Schinifoline Against Candida Albicans in Caenorhabditis Elegans

    Chengjie Shu1, Mengli Zhang1, Weiming Zhang1, Shihong Ma1, Guoqing Chen2 and Baojun Shi1,*

    Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 317-324, 2019, DOI:10.32604/phyton.2019.07766

    Abstract Zanthoxylum schinifolium has been used as spices and traditional medicine in China for hundreds of years. A variety of active substances have been isolated from Zanthoxylum schinifolium using biological and chemical techniques. Among these substances, the effect of schinifoline has gradually attracted much attention. Candida albicans is one of the most common pathogens isolated from the gastrointestinal tract, vagina, and mouth in healthy individuals. In a healthy population, there are various mechanisms in host, such as the microbial flora, the epithelial barriers, and the innate immune system, that can control the presence of Candida albicans.… More >

  • Open Access

    ARTICLE

    Antifungal activity of various essential oils against Saccharomyces cerevisiae depends on disruption of cell membrane integrity

    HATİCE BÜŞRA KONUK, BENGÜ ERGÜDEN*

    BIOCELL, Vol.41, No.1, pp. 13-18, 2017, DOI:10.32604/biocell.2017.41.013

    Abstract Antifungal activity and mode of action of nine essential oils (EOs) against S. cerevisiae cells were examined. Antifungal effects of commercial lemon peel, orange peel, tea tree, turpentine, rosemary, peppermint, thyme, oregano and clove oils were determined through Minimum Inhibitory Concentration (MIC), Minimum Fungicidal Concentration (MFC) and inhibition zone measurements. The most effective oil was turpentine oil. Orange peel, thyme and oregano oils were also effective, according to MIC and MFC. Inhibition zone measurements, also revealed oregano, orange peel, thyme, turpentine and clove oils as most efficient ones. Later, membrane damage of yeast cells was studied… More >

  • Open Access

    ARTICLE

    In vitro evaluation of antifungal activity of Agave (Agave scabra, Salm Dyck) extracts against post-harvest mushrooms

    González-Álvarez M, S Moreno-Limón*, SM Salcedo-Martínez, EC Pérez-Rodríguez

    Phyton-International Journal of Experimental Botany, Vol.84, No.2, pp. 427-434, 2015, DOI:10.32604/phyton.2015.84.427

    Abstract The agricultural sector, and particularly the horticultural production, has a singular importance in agriculture, considering that it ranks second on agricultural products, nationally and worldwide. Fungal diseases are one of the major causes of vegetable loss during storage, reducing their nutritional value, quality and sale price. Vegetables are usually exposed to diverse treatments with chemical products before storage; as a result, fungal populations develop an increased resistance over time becoming more difficult to control. Because of this, research efforts toward finding more suitable chemicals to control fungal diseases are needed. Natural extracts may be an… More >

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