Shah Mohammad Naimul Islam¹, Niloy Paul¹, Md. Mezanur Rahman², Md. Ashraful Haque¹, Md. Motiar Rohman³, Mohammad Golam Mostofa^4,*

Phyton-International Journal of Experimental Botany, Vol.92, No.5, pp. 1513-1537, 2023, DOI:10.32604/phyton.2023.025175

Abstract Drought is a severe environmental constraint, causing a significant reduction in crop productivity across the world. Salicylic acid (SA) is an important plant growth regulator that helps plants cope with the adverse effects induced by various abiotic stresses. The current study investigated the potential effects of SA on drought tolerance efficacy in two barley (Hordeum vulgare) genotypes, namely BARI barley 5 and BARI barley 7. Ten-day-old barley seedlings were exposed to drought stress by maintaining 7.5% soil moisture content in the absence or presence of 0.5, 1.0 and 1.5 mM SA. Drought exposure led to severe damage to both genotypes,… More >

Luli Li^1,2, Nigel G. Halford³, Huihui Wang⁴, Yingjie Zong¹, Zhenzhu Guo¹, Ruiju Lu¹, Chenghong Liu¹, Zhiwei Chen^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1275-1287, 2023, DOI:10.32604/phyton.2023.026946

Abstract Barley (Hordeum vulgare L.) is an important economic crop for food, feed and industrial raw materials. In the present research, 112 barley landraces from the Shanghai region were genotyped using genotyping-by-sequencing (GBS), and the genetic diversity and population structure were analyzed. The results showed that 210,268 Single Nucleotide Polymorphisms (SNPs) were present in total, and the average poly-morphism information content (PIC) was 0.1642. Genetic diversity and population structure analyses suggested that these barley landraces were differentiated and could be divided into three sub-groups, with morphological traits of row-type and adherence of the hulls the main distinguishing factors between groups. Genotypes… More >

Walid Soufan^*, Nasser A. Al-Suhaibani

Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1483-1493, 2022, DOI:10.32604/phyton.2022.020264

Abstract Cereal-legume intercropping plays a vital role in the subsistence food production system that prevails in the arid regions. It not only provides profitable crop productivity for agricultural communities but also plays an important role in improving soil fertility. Therefore, the present research was conducted to assess the effect of the organic and mineral fertilizers on the forage yield and nutritional value in barley-pea intercropping system. The results revealed that the quality of forage grass is significantly influenced by both organic and inorganic fertilizer. Thus, organic fertilizer application has significantly influenced the dry matter (DM), crude ash (CA), crude protein (CP),… More >

Longhua Zhou^1,2,#, Ting He^1,2,#, Jing Li^1,2,3, Guimei Guo^1,2, Yingbo Li^1,2, Hongwei Xu^1,2, Runhong Gao^1,2, Linli Huang^1,2, Yifei Wang^1,2, Ruiju Lu^1,2, Zhiwei Chen^1,2,*, Chenghong Liu^1,2,*

Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1419-1428, 2022, DOI:10.32604/phyton.2022.019912

Abstract Phytohormones play important roles in plant growth and development, and polyploids are thought to be an important method for plant breeding. However, the relationship between ploidy and phytohormone is still unclear. In this study, barley at three ploidy levels were produced by microspore culture. Therefore, we further analyzed the phytohormone content in the shoots and roots of the three kinds of barley materials to study the effect of ploidy on phytohormones accumulation and distribution. The results showed that Abscisic acid (ABA), gibberellin (GA), jasmonic acid (JA), auxin (IAA), salicylic acid (SA) and cytokinin (CTK) were successfully determined in shoots and… More >

Yingbo Li^1,2,#, Ting He^1,2,#, Guimei Guo^1,2, Hongwei Xu^1,2, Yingjie Zong^1,2, Shuwei Zhang^1,2, Ruiju Lu^1,2, Longhua Zhou^1,2,*, Chenghong Liu^1,2,*

Phyton-International Journal of Experimental Botany, Vol.91, No.6, pp. 1153-1164, 2022, DOI:10.32604/phyton.2022.019520

Abstract Agrobacterium-mediated transformation has been widely used in plants. However, the mechanism in plant cells’ response to Agrobacterium infection was very complex. The mechanism of the determinants in host cell remains obscure, especially in barley, which is recalcitrant for Agrobacterium-mediated transformation. In the present study, microspore-derived embryogenic calli (MDEC) from barley elite cultivar were employed as unique subjects to characterize the mechanisms during the Agrobacterium infection process. Hua 30 MDEC can be successfully infected by Agrobacterium. RNA-sequencing at different infection points (0, 2, 6, 12, 24 hpi) was performed. The average expressional intensity of the whole genomics increased from 0 to… More >

CÜNEYT UÇARLI^*

BIOCELL, Vol.45, No.6, pp. 1673-1685, 2021, DOI:10.32604/biocell.2021.016316

Abstract RAV (Related to ABI3/VP1) transcription factors are unique members of the AP2-ERF superfamily with AP2 and B3 domains and play important roles in the regulation of seed germination, plant growth, and stress response. In the study, 7 RAV genes, named HvRAVs, were identified in barley based on the available genome sequences. While five of the seven HvRAVs were located on chromosome 3, HvRAV5 and HvRAV7 were located on chromosome 1 and 4, respectively. Six of the predicted HvRAVs were intron-less, except HvRAV2, which had one intron. HvRAV proteins have shown basic, instable, and hydrophilic properties. The AP2 domain specific RAYD… More >

Mengyuan Dong^#, Rong Sun^#, Qianhui Yang, Lantian Zhang, Yangying Yong, Yunxia Fang, Xian Zhang, Xiaoqin Zhang^*, Dawei Xue^*

Phyton-International Journal of Experimental Botany, Vol.90, No.6, pp. 1589-1598, 2021, DOI:10.32604/phyton.2021.017465

Abstract In recent years, zinc oxide nanoparticles (ZnO NPs) have been widely used as zinc fertilizers and pesticides. The use of ZnO NPs in this way can provide benefits to humans, but also has potential risks. ZnO NPs inevitably enter the environment during their production and use, which affects the ecological environment and crop growth. In order to investigate the phenotype, physiology, and gene expression of barley (Hordeum vulgare L.) seedlings under ZnO NPs stress, the barely cultivars ZJU3 (P21), Golden Promise (GP) and L23 were chosen for study. Different ZnO NPs concentrations were applied to compare the physiological and biochemical… More >

Longhua Zhou^1,2,#, Ting He^1,2,#, Guimei Guo^1,2, Jing Li^1,2, Ruiju Lu^1,2, Yingbo Li^1,2,*, Chenghong Liu^1,2,*

Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 987-997, 2020, DOI:10.32604/phyton.2020.011728

Abstract Barley grain is a valuable source of β-glucan, which is an important component of dietary fiber with significant human health benefits. Although the genetic basis of β-glucan biosynthesis has been widely studied, a genome-wide association study (GWAS) is still required for a scan of the candidate genes related to the complex quantitative trait based on the high-quality barley reference genome. In this study, a GWAS was conducted using a population composed of 87 barley landraces (39 hulled and 48 hulless, β-glucan from 2.07% to 6.56%) with 191,098 nucleotide polymorphisms (SNPs) markers to cover the chromosomes with the highest density. The… More >

Lantian Zhang, Siyi Wang, Yuyu Chen, Mengyuan Dong, Yunxia Fang, Xian Zhang, Tao Tong, Ziling Zhang, Junjun Zheng, Dawei Xue^*, Xiaoqin Zhang^*

Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 229-251, 2020, DOI:10.32604/phyton.2020.10022

Abstract The F-box protein-encoding gene family plays an essential role in plant stress resistance. In present study, 126 non-redundant F-box genes were identified in barley (Hordeum vulgare L., Hv). The corresponding proteins contained 165– 887 amino acid residues and all were amphiphilic, except 5 proteins. Phylogenetic analysis of F-box protein sequences in barley and stress-related F-box protein sequences in wheat and Arabidopsis thaliana (At) was used to classify barley F-box genes are divided into 9 subfamilies (A–I). A structure-based sequence alignment demonstrated that F-box proteins were highly conserved with a total of 10 conserved motifs. In total, 124 F-box genes were… More >

Zepeda-Guzmán S¹, M Gómez-Romero², C Sosa-Aguirre¹, J Villegas¹

Phyton-International Journal of Experimental Botany, Vol.87, pp. 183-190, 2018, DOI:10.32604/phyton.2018.87.183

Abstract Changes in root architecture are a strategy used by plants to explore the soil for available resources. The presence of beneficial microorganisms in the rhizosphere as well as plant growth regulators can cause changes in root development and promote the availability of water and nutrients. The effect of microorganisms or growth regulators on plant growth has been tested, but little is known about the effect they have on the architecture of the root of Hordeum vulgare L. Therefore the objective of this study was to evaluate the effect of the application of Rhizoglomus intraradices, Azospirillum brasilense, quercetin and epibrassinolide, alone… More >