Juanita BUSTAMANTE1,*, Analía CZERNICZYNIEC2, Silvia LORES-ARNAIZ2
BIOCELL, Vol.40, No.1, pp. 11-14, 2016, DOI:10.32604/biocell.2016.40.011
Abstract The suppressive effects of ketamine on intracellular calcium has been reported in a variety of cells
although the mechanisms involved are not well understood. The aim of this work was to evaluate the ketamine
effect on the mitochondrial Ca2+ accumulation and the cellular Ca2+ mobilization using FLUO4-AM and flow
cytometry. The results showed that mitochondria from ketamine injected animals presented a lower ability to
retain calcium at concentrations higher than 20 μM, as compared with controls (saline injected animals). In addition, ketamine showed a significant decreased KCl-induced intracellular calcium concentration. KCl increased
calcium influx through cellular depolarization. According to the… More >
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