Xiaotong Wei^1,#, Xuhong Fan^2,#, Xuan Lu¹, Liyuan Han¹, Honglin Zhang³, Siyan Liu³, Jing Qu³, Shuyan Guan^3,*, Yiyong Ma^3,*

Phyton-International Journal of Experimental Botany, Vol.91, No.9, pp. 1945-1957, 2022, DOI:10.32604/phyton.2022.020062

Abstract

The embryos of maize (Zea mays L.) inbred lines GS02, GS07, GS08, GS11 and GS15 were used as receptor materials to optimize the receptor system from the aspects of genotype, medium components and stress (PEG6000, mannitol, salt and low phosphorus). The results showed that GS07 had the highest induction rate (95.2%). Orthogonal test analysis showed that the best combination of medium components in induction was A₂B₃C₁D₃₍₂₎, namely, the concentration of 2, 4-dichlorophenoxy acetic acid (2,4-D) was 4 mg·mL^-1, the concentration of L-Proline (L-Pro) was 0.8 mg·mL^-1, and the concentration of silver nitrate (AgNO₃) was 10 mg·mL^-1 (or 5 mg·mL). Interestingly,… More >

Lucía Isabel Chávez-Ortiz¹, José Francisco Morales-Domínguez¹, Araceli Rodríguez-Sahagún², Eugenio Pérez-Molphe-Balch^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 1003-1013, 2021, DOI:10.32604/phyton.2021.012862

Abstract Agave guiengolaGentry is an endemic plant from a very small locality in Oaxaca, Mexico. Its conservation status is fragile and can rapidly worsen. Because of its scarcity, this agave has been used solely for ornamental purposes, but it could have other uses if more plants were available. In vitro propagation by enhanced axillary sprouting from stem segments was attained using Murashige and Skoog Basal Medium (MS) as well as basal medium supplemented with cytokinins 6-Benzylaminopurine (BA) or 6-(γ,γ-Dimethylallylamino)purine (2iP). The best treatment for shoot induction in semisolid medium consisted in MS supplemented with 2 mg l^–1 BA, obtaining a mean… More >

Peng Jiao^1,#, Ruiqi Ma^2,#, Zhuo Qi¹, Zhenzhong Jiang¹, Siyan Liu¹, Jing Qu³, Shuyan Guan^1,*, Yiyong Ma^3,*

Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 121-130, 2020, DOI:10.32604/phyton.2020.07980

Abstract This research uses the immature embryos of inbred maize lines (GSH9901, Hi01, Hi02, and Chang 7-2) as receptor materials to establish the callus induction system. These inbred lines provide the receptor materials for the genetic regeneration of maize and the verification of the genetic functions of maize. The factor experiment and orthogonal experiments were used to investigate the impacts of different genotypes, immature embryo size, shield orientation, 2, 4-D concentration, proline concentration, and folic acid concentration on the induction rate of embryogenic callus tissue. A sensitivity experiment testing glyphosate (Bar) and an antibiotic (Cefotaxime sodium) were also conducted. The results… More >