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  • Open Access


    In Vitro Propagation and Artificial Seed Production of Fritillaria cirrhosa D. Don, an Endangered Medicinal Plant

    Qian Tao, Guiqi Han, Bujin Ma, Hongmei Jia, Can Zhao, Wenshang Li, Zhuyun Yan*

    Phyton-International Journal of Experimental Botany, Vol.93, No.6, pp. 1297-1310, 2024, DOI:10.32604/phyton.2024.051923

    Abstract Fritillaria cirrhosa D. Don (Liliaceae) is an endangered perennial bulbous plant and its dry bulb is a valuable medicinal material with antitussive and expectorant effects. Nevertheless, lack of resources and expensive prices make it difficult to meet clinical needs. This study presents a regeneration system aimed at overcoming the challenge of inadequate supply in F. cirrhosa, focusing on: (1) callus induction, (2) bulblets and adventitious bud induction, and (3) artificial seed production. Callus development was achieved in 84.93% on Murashige and Skoog (MS) medium fortified with 1.0 mg·L picloram. The optimal medium for callus differentiation into regenerated… More >

  • Open Access


    Optimization of Callus Induction Conditions from Immature Embryos of Maize under Stress

    Xiaotong Wei1,#, Xuhong Fan2,#, Xuan Lu1, Liyuan Han1, Honglin Zhang3, Siyan Liu3, Jing Qu3, Shuyan Guan3,*, Yiyong Ma3,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.9, pp. 1945-1957, 2022, DOI:10.32604/phyton.2022.020062


    The embryos of maize (Zea mays L.) inbred lines GS02, GS07, GS08, GS11 and GS15 were used as receptor materials to optimize the receptor system from the aspects of genotype, medium components and stress (PEG6000, mannitol, salt and low phosphorus). The results showed that GS07 had the highest induction rate (95.2%). Orthogonal test analysis showed that the best combination of medium components in induction was A2B3C1D3(2), namely, the concentration of 2, 4-dichlorophenoxy acetic acid (2,4-D) was 4 mg·mL-1, the concentration of L-Proline (L-Pro) was 0.8 mg·mL-1, and the concentration of silver nitrate (AgNO3) was 10 mg·mL-1 (or 5 mg·mL).

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  • Open Access


    In Vitro Propagation of Agave guiengola Gentry Using Semisolid Medium and Temporary Immersion Bioreactors

    Lucía Isabel Chávez-Ortiz1, José Francisco Morales-Domínguez1, Araceli Rodríguez-Sahagún2, Eugenio Pérez-Molphe-Balch1,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 1003-1013, 2021, DOI:10.32604/phyton.2021.012862

    Abstract Agave guiengolaGentry is an endemic plant from a very small locality in Oaxaca, Mexico. Its conservation status is fragile and can rapidly worsen. Because of its scarcity, this agave has been used solely for ornamental purposes, but it could have other uses if more plants were available. In vitro propagation by enhanced axillary sprouting from stem segments was attained using Murashige and Skoog Basal Medium (MS) as well as basal medium supplemented with cytokinins 6-Benzylaminopurine (BA) or 6-(γ,γ-Dimethylallylamino)purine (2iP). The best treatment for shoot induction in semisolid medium consisted in MS supplemented with 2 mg l–1 BA, obtaining… More >

  • Open Access


    Optimization of Callus Induction Conditions from Immature Embryos in Maize and Plant Regeneration

    Peng Jiao1,#, Ruiqi Ma2,#, Zhuo Qi1, Zhenzhong Jiang1, Siyan Liu1, Jing Qu3, Shuyan Guan1,*, Yiyong Ma3,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 121-130, 2020, DOI:10.32604/phyton.2020.07980

    Abstract This research uses the immature embryos of inbred maize lines (GSH9901, Hi01, Hi02, and Chang 7-2) as receptor materials to establish the callus induction system. These inbred lines provide the receptor materials for the genetic regeneration of maize and the verification of the genetic functions of maize. The factor experiment and orthogonal experiments were used to investigate the impacts of different genotypes, immature embryo size, shield orientation, 2, 4-D concentration, proline concentration, and folic acid concentration on the induction rate of embryogenic callus tissue. A sensitivity experiment testing glyphosate (Bar) and an antibiotic (Cefotaxime sodium)… More >

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