Mutong Li^1,#, Xiaolian Yang^1,#, Zhishun Yu¹, Tingting Yuan¹, Wenjie Wang¹, Suqin Zhang^1,2,*, Guangdong Geng^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.10, pp. 3175-3188, 2025, DOI:10.32604/phyton.2025.070731 - 29 October 2025

Abstract Wheat yield mainly depends on leaf photosynthesis and grain carbohydrate accumulation. The aux/indole-3-acetic acid 13-like (Aux/IAA13L) gene was successfully cloned from Tritipyrum ‘Y1805’ and transformed into common wheat. A bioinformatics analysis showed that the TtAux/IAA13L protein, encoding 232 amino acids, was hydrophilic and unstable. TtAux/IAA13L and Tel5E01G609500 were grouped together in a phylogenetic tree. The TtAux/IAA13L expression levels in the overexpression lines were higher than in the wild-type (WT) plants at five developmental stages: tillering, elongation, heading, flowering, and grain-filling. The expression levels in the overexpression lines first increased, peaked at the flowering stage, and then decreased.… More >

Anton Louise P. De Ocampo^*

Revue Internationale de Géomatique, Vol.34, pp. 731-749, 2025, DOI:10.32604/rig.2025.066185 - 10 October 2025

Abstract The effects of climate change are becoming more evident nowadays, and the environmental stress imposed on crops has become more severe. Farmers around the globe continually seek ways to gain insights into crop health and provide mitigation as early as possible. Phenotyping is a non-destructive method for assessing crop responses to environmental stresses and can be performed using airborne systems. Unmanned Aerial Systems (UAS) have significantly contributed to high-throughput phenotyping and made the process rapid, efficient, and non-invasive for collecting large-scale agronomic data. Because of the high complexity and cost of specialized equipment used in… More >

Qingsong Jiao, Xueyun Hu^*

Phyton-International Journal of Experimental Botany, Vol.94, No.9, pp. 2615-2630, 2025, DOI:10.32604/phyton.2025.069246 - 30 September 2025

Abstract Chlorophyll fluorescence, particularly the parameter F_v/F_m, has emerged as a reliable, non-invasive indicator of the maximum quantum efficiency of Photosystem II (PSII) in plants. Over the past decade, significant research has leveraged F_v/F_m to evaluate plant responses to a wide range of biotic and abiotic stresses, as well as to support crop improvement and ecological monitoring. This review synthesizes recent progress in understanding the physiological basis, measurement techniques, and applied significance of F_v/F_m across diverse plant systems. We highlight methodological advancements in fluorescence imaging and remote sensing, identify consistent patterns and contrasting findings in stress-response studies, and More >

Farhat Abbas¹, Fahim Ullah Khan^1,2,*, Salem Al-Naemi³, Awni Al-Otoom¹, Ahmed T. Moustafa⁴, Khaled Shami¹

Phyton-International Journal of Experimental Botany, Vol.94, No.9, pp. 2911-2925, 2025, DOI:10.32604/phyton.2025.064239 - 30 September 2025

Abstract This study aimed to explore the relationship between Soil-Plant Analysis Development (SPAD) values and key environmental factors in cucumber (Cucumis sativus L.) cultivation in a greenhouse. SPAD values, indicative of chlorophyll content, reflect plant health and productivity. The analysis revealed strong positive correlations between SPAD values and both indoor light intensity (ILI, r = 0.59, p < 0.001) and outdoor light intensity (OLI, r = 0.62, p < 0.001), suggesting that higher light intensities were associated with enhanced SPAD values. In contrast, significant negative correlations were found between SPAD values and soil temperature at 15–30 cm depth… More >

Lina Song¹, Caijie Yi¹, Shiwei Zhao¹, Yuxin Peng¹, Zijing Li¹, Yuqiang Zhang ², Hua Zhang¹, Helan Qin¹, Huali Zhang^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.8, pp. 2593-2613, 2025, DOI:10.32604/phyton.2025.068133 - 29 August 2025

Abstract Naturally occurring yellow leaf mutants are an important resource for studying pigment content and biosynthesis, as well as related gene expression. In our ongoing cultivation of Rehmannia chingii H. L. Li, we found an off-type yellow plant. The yellowing started with the new leaves and gradually spread downward until the entire plant exhibited a stable shade of yellow. We studied the differences in the chlorophyll and carotenoid content, carotenoid profile, and transcriptome of this yellow-leaf mutant (P2). Compared to the wild-type R. chingii plant (P1), P2 leaves had significantly lower chlorophyll and carotenoid content. LC-MS/MS analysis revealed More >

Jinge Li^1,2, Chuyao Wang^1,2, Fangbo Cao^1,2, Min Huang^1,2,*, Jiana Chen^1,2,*

Phyton-International Journal of Experimental Botany, Vol.94, No.7, pp. 2155-2165, 2025, DOI:10.32604/phyton.2025.066940 - 31 July 2025

Abstract Grain filling is a critical determinant of yield and quality in rice. This study aims to clarify the association between grain photosynthesis and the filling rate of rice varieties with different grain weights, providing a theoretical foundation for optimizing grain-filling processes. Two rice varieties with similar growth duration but different grain weights were selected: a large-grain variety, Lingliangyou 268 (L268), and a small-grain variety, Ruiliangyou 1053 (R1053). Differences in grain filling, grain photosynthetic rate, and grain chlorophyll content were systematically examined during the filling stage. Results showed significant differences in grain-filling, grain photosynthetic rate, and… More >

Antonela Markulj Kulundžić^1,*, Daniela Horvat², Marija Kovačević Babić², Anto Mijić¹, Aleksandra Sudarić¹, Maja Matoša Kočar¹, Tomislav Duvnjak¹, Ivica Liović¹, Ivana Varga³, Marija Viljevac Vuletić^2,*

Phyton-International Journal of Experimental Botany, Vol.94, No.7, pp. 2075-2095, 2025, DOI:10.32604/phyton.2025.065961 - 31 July 2025

Abstract Sunflower leaf photosynthesis strongly depends on the leaf position in the plant stem conditioning, which directly affects other physiological processes. Therefore, a study of the leaf’s physiological status regarding the leaf position in the stem was performed on sunflowers in the flowering stage. Eight differently positioned leaves were investigated, starting with the youngest leaf on the top of the stem to the leaves of the stem bottom, assigned as the oldest senescent leaves. According to chlorophyll fluorescence (ChlF) parameters connected to photosystem II (PSII) processes, significant changes in PSII functioning occurred only in the senescent… More >

Zhipeng Sheng, Zhaoyu Hou, Jun Tao, Daqiu Zhao^*

Phyton-International Journal of Experimental Botany, Vol.94, No.6, pp. 1751-1767, 2025, DOI:10.32604/phyton.2025.065874 - 27 June 2025

Abstract Summer high temperatures have severely impaired the growth of herbaceous peony (Paeonia lactiflora Pall.) in East China. While compound fertilizer application enhances soil fertility and promotes plant growth, its efficacy in maintaining optimal plant performance under summer heat stress remains poorly understood. This study investigated the effects of compound fertilizer application on herbaceous peony growth during summer thermal stress. Results demonstrated that compound fertilizer supplementation significantly improved plant growth under elevated temperatures, manifesting enhanced phenotypic characteristics, elevated antioxidant enzyme activities, and increased nutrient accumulation. Compared to untreated controls, fertilized plants exhibited three key responses: (1) increased More >

Kesu Wei¹, Guangju Liu², Bin Wei³, Qifang Zhang⁴, Shengjiang Wu¹, Zhaowei Li^2,*

Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1287-1298, 2025, DOI:10.32604/phyton.2025.062385 - 30 April 2025

Abstract To systematically examine the tolerance of flue-cured tobacco K326 to soil drought stress, this study set up different water content trials for K326 at 60%, 40%, and 20% levels during the vigorous growing period by greenhouse pot planting. Pigment content, chlorophyll fluorescence parameters, antioxidant enzyme activity, and SPS and GS activities associated with carbon and nitrogen metabolism were investigated in the K326 tobacco leaves. The results showed that chlorophyll a and chlorophyll b contents decreased, non-photochemical quenching coefficient Y(NPQ) decreased in the later stage of vigorous growth, and energy dissipation quantum yield Y(NO) levels increased… More >

Alexis Salazar-Navarro¹, Víctor Ruíz-Valdiviezo², José Joya-Dávila¹, Daniel González-Mendoza^1,*, Olivia Tzintzun-Camacho¹, Ulin Basilio-Cortes¹, Dagoberto Duran-Hernández¹, Henry López-Lopez¹, Onecimo Grimaldo-Juárez¹

Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 641-655, 2025, DOI:10.32604/phyton.2025.062871 - 31 March 2025

Abstract The biochemical response of Coffea arabica var. Borbon to chitosan and chitosan oligosaccharides (COS) was evaluated in one-year-old plants under greenhouse conditions. COS solutions were synthesized through chemical and physical hydrolysis using acetic acid, hydrogen peroxide, and microwave irradiation. The obtained COS had an average molecular weight (Mw) of 3549.90 ± 0.33 Daltons (Da), a deacetylation degree (DD) of 76.64 ± 1.12%, and a polymerization degree (PD) of 18.91 ± 0.0018. Solutions of chitosan and COS were applied to C. arabica var. Borbon at concentrations of 0.25, 0.5, and 1 wt%. The experimental design was conducted using… More >