Open Access

REVIEW

Recent Advances and Emerging Trends in Chlorophyll Fluorescence Parameter F_v/F_m

Qingsong Jiao, Xueyun Hu^*

College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, 225009, China

* Corresponding Author: Xueyun Hu. Email:

Phyton-International Journal of Experimental Botany 2025, 94(9), 2615-2630. https://doi.org/10.32604/phyton.2025.069246

Received 18 June 2025; Accepted 07 August 2025; Issue published 30 September 2025

Abstract

Chlorophyll fluorescence, particularly the parameter F_v/F_m, has emerged as a reliable, non-invasive indicator of the maximum quantum efficiency of Photosystem II (PSII) in plants. Over the past decade, significant research has leveraged F_v/F_m to evaluate plant responses to a wide range of biotic and abiotic stresses, as well as to support crop improvement and ecological monitoring. This review synthesizes recent progress in understanding the physiological basis, measurement techniques, and applied significance of F_v/F_m across diverse plant systems. We highlight methodological advancements in fluorescence imaging and remote sensing, identify consistent patterns and contrasting findings in stress-response studies, and explore how F_v/F_m correlates with other physiological and molecular indicators. Despite its widespread use, challenges remain regarding data interpretation, environmental variability, and integration with multi-parameter models. This review also addresses critical knowledge gaps and proposes future research directions, including AI-driven analysis, a least-squares support vector machine (LSSVM) model, and high-resolution phenotyping. By consolidating findings from recent literature, this work provides a comprehensive reference for researchers aiming to harness F_v/F_m in both basic and applied plant sciences.

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Keywords

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